Study of senescence in old cultures of the Bactris gasipaes Kunth in vitro

Graner, Edgard; Brondani, Gilvano Ebling; Almeida, Cristina Vieira de; Batagin-Piotto, Katherine Derlene; Almeida, Marcílio de

doi:10.1007/s11240-014-0672-4

Cited by 10 publications

(6 citation statements)

References 53 publications

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“…Other constraints in tissue culture collections are the occurrence of cellular ageing and senescence during prolonged cultivation. In eight-year-old peach palm (Bactris gasipaes) cultures, processes of plant cell death and senescence were visible through nuclear condensation, cell degradation and the development of large intercellular spaces [68]. The effect of cellular ageing or senescence may appear in parallel with slow growing endophytic microbes that can accumulate over time.…”

Section: Problems Associated With In Vitro Collectionsmentioning

confidence: 99%

Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen

Panis

Nagel

houwe

2020

Plants

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The conservation of crop genetic resources, including their wild relatives, is of utmost importance for the future of mankind. Most crops produce orthodox seeds and can, therefore, be stored in seed genebanks. However, this is not an option for crops and species that produce recalcitrant (non-storable) seeds such as cacao, coffee and avocado, for crops that do not produce seeds at all; therefore, they are inevitably vegetatively propagated such as bananas, or crops that are predominantly clonally propagated as their seeds are not true to type, such as potato, cassava and many fruit trees. Field, in vitro and cryopreserved collections provide an alternative in such cases. In this paper, an overview is given on how to manage and setup a field, in vitro and cryopreserved collections, as well as advantages and associated problems taking into account the practical, financial and safety issues in the long-term. In addition, the need for identification of unique accessions and elimination of duplicates is discussed. The different conservation methods are illustrated with practical examples and experiences from national and international genebanks. Finally, the importance of establishing safe and long-term conservation methods and associated backup possibilities is highlighted in the frame of the global COVID-19 pandemic.

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Section: Problems Associated With In Vitro Collectionsmentioning

confidence: 99%

Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen

Panis

Nagel

houwe

2020

Plants

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“…However, genes associated with PCD are differentially regulated in dPCD and ePCD, allowing the separation of these two types of PCD [ 9 ]. Stress induced by in vitro culture, mostly long-term cultivation and exposure to plant growth regulators, activates PCD-related genes and initiates the PCD process [ 30 , 31 , 32 ].…”

Section: Developmental Versus Environmental Pcd In Plant Cells—genmentioning

confidence: 99%

Insights into Plant Programmed Cell Death Induced by Heavy Metals—Discovering a Terra Incognita

Sychta

Słomka

Kuta

2021

Cells

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Programmed cell death (PCD) is a process that plays a fundamental role in plant development and responses to biotic and abiotic stresses. Knowledge of plant PCD mechanisms is still very scarce and is incomparable to the large number of studies on PCD mechanisms in animals. Quick and accurate assays, e.g., the TUNEL assay, comet assay, and analysis of caspase-like enzyme activity, enable the differentiation of PCD from necrosis. Two main types of plant PCD, developmental (dPCD) regulated by internal factors, and environmental (ePCD) induced by external stimuli, are distinguished based on the differences in the expression of the conserved PCD-inducing genes. Abiotic stress factors, including heavy metals, induce necrosis or ePCD. Heavy metals induce PCD by triggering oxidative stress via reactive oxygen species (ROS) overproduction. ROS that are mainly produced by mitochondria modulate phytotoxicity mechanisms induced by heavy metals. Complex crosstalk between ROS, hormones (ethylene), nitric oxide (NO), and calcium ions evokes PCD, with proteases with caspase-like activity executing PCD in plant cells exposed to heavy metals. This pathway leads to very similar cytological hallmarks of heavy metal induced PCD to PCD induced by other abiotic factors. The forms, hallmarks, mechanisms, and genetic regulation of plant ePCD induced by abiotic stress are reviewed here in detail, with an emphasis on plant cell culture as a suitable model for PCD studies. The similarities and differences between plant and animal PCD are also discussed.

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“…形态学方法中最为可靠的手段之一 [1] , 可以在电镜下观察液泡结合激光扫描共聚焦显微镜下的TUNEL阳性判明液泡型PCD存在 [30,48] , 此外还可以在电镜下图 1 (网络版彩图)DAPI标记降解细胞核和标记全部细胞核的示例((a)和(b)分别引自文献 [34]和 [35]). (a) DAPI在烟草细胞培养液中标记异常细胞核 [34] , 白色箭头指示正在降解的细胞核, 比未降解的细胞核亮度更大或出现了空心化, *指示已经解体的细胞核; (b) DAPI在西红柿 (Lycopersicon esculintum)胚发育子叶折叠阶段中标记全部细胞核 [35] , 以证实(b)中左图的TUNEL未标记的细胞核是存在从而排除假阴性.…”

Section: 电子显微镜(以下简称电镜)观察仍然是目前的unclassified

“…同样取得了重大的研究进展 [72,73] , 结果和种子保存相似, 离体保存的组织或细胞的生活力减退与发生 PCD的细胞数量成正相关. Wesley-Smith等人 [72] 等提出了低温保存的冰晶损伤对组织的影响主要是以 Programmed cell death (PCD) is essential to the survival and evolution of plants.…”

Section: 除了种子离体保存的胞组织和细胞中的Pcdunclassified

Identifying stress-related programmed cell death improves research on plant resistance

Chen

et al. 2016

Chin. Sci. Bull.

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Study of senescence in old cultures of the Bactris gasipaes Kunth in vitro

Cited by 10 publications

References 53 publications

Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen

Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen

Insights into Plant Programmed Cell Death Induced by Heavy Metals—Discovering a Terra Incognita

Identifying stress-related programmed cell death improves research on plant resistance

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