Study of Self-Association of Human CstF-64 RNA Recognition Motif

Abstract

present continuous wave (cw) EPR and Double Electron-Electron Resonance (DEER) kinetics, on two crucial steps of pore formation: shell opening and linker contraction of TcA. Both steps are triggered by basic pH and proceed slowly with half-lives in the 10 hours range, with no indications of enrichment of an open, non-contracted intermediate. Moreover, we found that mutations in the TcA subunit, which prevent membrane insertion, caused an acceleration of pore formation by at least three orders of magnitudes and modified the pH-dependency of the reaction such that pores are also formed at acidic pH values in vitro. A new cryo-EM structure of the membraneintegration-deficient TcA variant shows a slight opening of the outer shell, which indicates that a structural rearrangement that might be caused by receptor binding in vivo, must precede the prepore-to-pore transition to effectively activate Tc.

Plant 3’ Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression

“…An interaction between CstF64 and CPSF160 proved to be necessary to define the cleavage site (Chan et al, 2011). CstF64 is directly involved in the recognition of PAS and in the global regulation of APA, being able to binding to G/U-rich sequences downstream of the PAS, and its specificity dependent of the interaction with CPSF (Yao et al, 2012;Masoumzadeh et al, 2020). Also, CstF77 increases the affinity of CstF64 for RNA targets, recruiting CstF50, that is involved in the recognition of G/U-rich sequences (Yang et al, 2018).…”