Study of Protein Components of Fish Skin Mucus with Thrombogenic Activity

Fomina, L. L.; Oshurkova, Yu. L.; Junina, O. A.; Kulakova, T. S.; Weitzel, A. E.

doi:10.3103/s1068367420040060

Cited by 2 publications

(1 citation statement)

References 5 publications

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“…As a result, aquatic-derived bioactive compounds have been reported to play a critical role to human health and nutrition. Notable examples include fish-derived clotting factors (Fomina et al, 2020), Seaweed antioxidants (Zerrifi et al, 2018), common water hyacinth, Eichhornia crassipes anticancer phytochemicals (Mtewa et al, 2021), anti-obesity polysaccharides from red algae (Yang et al, 2019), novel conopeptides from marine snails (Zhang et al, 2021), antiinflammatory cembranoids from soft coral (Peng et al, 2020), novel AMPs from sea weed, Porphyra yezoensis (Jiao et al, 2019), anchovy fish-derived antioxidant peptides (Najafian and Babji, 2018), Marine-derived Penicillium purpurogenum anti-tumor metabolites (Teles et al, 2020), Salmon calcitonin anti-glycemic and anti-osteoporosis peptides (Chesnut et al 2008), and fish-derived CF-14 AMPs (Li et al, 2019) among others. Despite, these tremendous contributions to drug leads, majority of the studies seem to focus much few highly valued species of marine habitats, with far limited emphasis on freshwater biochemical compounds.…”

Section: Discussionmentioning

confidence: 99%

Identification of Antimicrobial Peptides Isolated From the Skin Mucus of African Catfish, Clarias gariepinus (Burchell, 1822)

et al. 2021

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Antimicrobial peptides (AMPs) constitute a broad range of bioactive compounds in diverse organisms, including fish. They are effector molecules for the innate immune response, against pathogens, tissue damage and infections. Still, AMPs from African Catfish, Clarias gariepinus, skin mucus are largely unexplored despite their possible therapeutic role in combating antimicrobial resistance. In this study, African Catfish Antimicrobial peptides (ACAPs) were identified from the skin mucus of African Catfish, C. gariepinus. Native peptides were extracted from fish mucus scrapings in 10% acetic acid (v/v) and ultra-filtered using 5 kDa molecular weight cut-off membrane. The extract was purified using C18 Solid-Phase Extraction. The antibacterial activity was determined using the Agar Well Diffusion method and broth-dilution method utilizing Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922). Thereafter, Sephadex G-25 gel filtration was further utilized in bio-guided isolation of the most active fractions prior to peptide identification using Orbitrap Fusion Lumos Tribrid Mass Spectrometry. The skin mucus extracted from African Catfish from all the three major lakes of Uganda exhibited antimicrobial activity on E. coli and S. aureus. Lake Albert’s C. gariepinus demonstrated the best activity with the lowest MIC of 2.84 and 0.71 μg/ml on S. aureus and E. coli, respectively. Sephadex G-25 peak I mass spectrometry analysis (Data are available via ProteomeXchange with identifier PXD029193) alongside in silico analysis revealed seven short peptides (11–16 amino acid residues) of high antimicrobial scores (0.561–0.905 units). In addition, these peptides had a low molecular weight (1005.57–1622.05 Da) and had percentage hydrophobicity above 54%. Up to four of these AMPs demonstrated α-helix structure conformation, rendering them amphipathic. The findings of this study indicate that novel AMPs can be sourced from the skin mucus of C. gariepinus. Such AMPs are potential alternatives to the traditional antibiotics and can be of great application to food and pharmaceutical industries; however, further studies are still needed to establish their drug-likeness and safety profiles.

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