Study of platelet indices in dengue fever with thrombocytopenia and correlation of immature platelet fraction (IPF) with platelet recovery

Abstract

Recently, novel platelet indices such as Mean Platelet Volume (MPV), Platelet Distribution Width (PDW), and Platelet to Large Cell Ratio (PLCR) have been investigated as prospective platelet activation markers [4]. Mean Platelet Volume is a surrogate marker of bone marrow activity; a high MPV indicates increased megakaryocyte activity. A low MPV indicates marrow suppression. Platelets with increased number and size of pseudopodia differ in size, possibly affecting platelet distribution width (PDW) which increases during platelet activation [4]. Platelet to Large Cell Ratio (PLCR) is signifi cantly decreased in patients with thrombocytosis than in normal while it is increased in thrombocytopenia. PLCR was inversely related to platelet count and directly related to PDW and MPV [5]. The immature platelet fraction (IPF) is a new parameter which is an automated measure of reticulated platelets in peripheral

Correlation of Platelet Parameters With the Severity of Thrombocytopenia in Dengue Fever in Children Aged Less Than 18 Years at a Tertiary Care Centre: A Cross-Sectional Study

“…A robust correlation was found between low MPV (<9 fL) and low platelet count, mirroring our study's findings [ 12 ]. We noticed a correlation coefficient (r) of 0.124 and a p-value of <0.05 [ 1 ]. The normal value of PDW is between 8.3 fL and 56.6 fL.…”

“…Thrombocytopenia is the most common laboratory finding. Thrombocytopenia in dengue is due to the suppression of bone marrow, platelet destruction by antibodies to the dengue virus, excessive consumption of platelets, viral replication-mediated destruction of platelets by complement-mediated lysis, and apoptosis [ 1 ]. In response to thrombocytopenia, bone marrow is activated and more giant cells are produced, which is reflected as an increase in mean platelet volume (MPV), which is a major response of bone marrow to thrombocytopenia [ 2 ].…”

Evaluation of clinical and laboratory characteristics of dengue viral infection and risk factors of dengue hemorrhagic fever: a multi-center retrospective analysis

“…Clinical case definition of dengue viral infection is suspected dengue infection was defined as the presence of fever and any two of the below mentioned symptoms fever, myalgia, arthralgia, headache, skin rash, retro-orbital pain, hemorrhagic manifestation(s) [ 15 ]. Probable dengue infection was defined as the presence of low in white blood cell count ˂ 4000 and low in platelet cells count ˂ 100,000 [ 16 ] or hematocrit ˂ 30% or ˃ 55%. Confirmed case for the dengue viral infection was defined as positive NS-1 or viral detection by PCR or seroconversion from negative for dengue virus specific IgM antibody in acute phase (˂ 5days after onset of symptoms) to positive for dengue virus specific IgM antibody in convalescent phase specimen collected ≥ 5 days after onset of symptoms or ≥ 4-fold rise in titer of IgG in paired acute and convalescent serum sample [ 17 ].…”