Study of pancreatic lipase inhibition kinetics and LC–QTOF–MS‐based identification of bioactive constituents of Momordica charantia fruits

Abstract

The different parts of Momordica charantia have been reported to have several therapeutic applications against hyperglycemia and hypercholesterolemia associated with pancreatic lipase (PL). Inhibition of this enzyme prevents the absorption of dietary triglyceride in the intestine, and thus exerts an anti‐obesity effect. This study aimed to investigate the bioactive constituents of the fruits of M. charantia (MCF) extract and fractions against pancreatic PL followed by study of their inhibition kinetics. The PL inhibitory assay was performed spectrophotometrically by measuring the change in absorbance of the products at 405 nm, using p‐nitrophenylcaprylate as substrate. The results indicated that the ethyl acetate fraction of MCF (EFMC) offered significant, dose‐dependent inhibition against PL, compared with the positive control, Orlistat. The enzyme kinetics study revealed the inhibition to be a mixed type in nature. Additionally, the total phenol and flavonoid content of the fractions was estimated. A positive correlation between phenolic content of EFMC and its PL inhibitory activity was established statistically, which implied that higher inhibition potential was contributed by the phenolic compounds. The identification of the bioactive constituents was further confirmed by LC–QTOF–MS study. This finding suggested that phenolic compounds of MCF can serve as functional food components to address obesity‐related disorders linked with PL.

High‐performance thin layer chromatography method development, validation, and assessment of enzymatic inhibition potential of Potentilla fulgens hydroalcoholic roots extract

“…The total phenolic content of the hydroalcoholic extract of Potentilla fulgens root was determined using the Folin‐Ciocalteau following the method of Chanda et al. [24] with some modifications. In brief, 20 μl aliquot of extract (1 mg/ml in methanol) was added with 90 μl of 10% Folin‐Ciocalteu's reagent followed by 90 μl of 7.5% NaHCO 3 .…”

“…With minor changes, the total flavonoid content of the extract was analyzed using the aluminum chloride method following Chanda et al [24]. Catechin (20-100 μg/ml) was used as a standard drug to prepare the calibration curve.…”

Momordica charantia L. improves airway hyperresponsiveness and suppresses inflammation in a murine model of allergic asthma

“…The capillary voltage was set at 3000 V; nozzle voltage at 500 V; gas temperature at 350°C; drying gas,13 L min −1 ; nebulizer, 60 psi; sheaf gas, 300°C; fragmentor, 140 V; skimmer, 80 V; and collision energy voltage 10-at 30 V. The LC-MS data were processed using the software Qualitative Navigator B.08.00 and Qualitative Workflows B.08.00. [6][7][8]…”

The Effect of Momordica charantia in the Treatment of Diabetes Mellitus: A Review

“…It has been proved that the inhibitory activity of MC ethanol extract on pancreatic lipase and α -glucosidase is performed in a dose-dependent manner [ 49 ]. Moreover, the suppression of the ethyl acetate fraction of MC extract on pancreatic lipase is positively correlated with its phenol content [ 50 ]. Momordicin, a compound identified from MC, acted against α -amylase with IC50 of 15.86 μ g/ml [ 51 ].…”