Study of multivariate analysis of quantitative traits in Iranian pumpkin lines

Yadegari, Mehrab; Ansari, Mohammad Hossein

doi:10.2298/gensr1702425y

Cited by 5 publications

(6 citation statements)

References 12 publications

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“…This method is a relatively not expensive and is powerful way of measuring allele frequencies of a particular gene. Seed storage protein patterns observed in electrophoretogram developed for various plant seeds have been widely used for assessing genetic diversity (Petrovic et al, 2017;Yadegari and Ansari, 2017;Saroei et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Genetic diversity is very important in plant propagation for growing fresh and high yielding varieties of cereal crops that are enable to defend them against diseases and to tolerate abiotic stresses (Yadegari and Ansari, 2017). In the evolutionary prospective of a species the determination of the genetic diversity is a key factor and is the most important point in research in the field of genetics that enable us to find out difference both domestic and natural populations and to find out the probable mechanisms of variations amongst the individuals of a population (Petrovic et al, 2017;Yadegari and Ansari, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Description of germplasm by using biochemical markers have got distinct attention due to its wide use in crop development and the assortment of desired genotypes for crop/plant breeding. To resolve the evolutionary and taxonomic complications of various crop/plants, the genetic marker and protein sketching has been effectively used (Petrovic et al, 2017;Yadegari and Ansari, 2017;Saroei et al, 2017). Different protocols were used to optimize the Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis for Acacia modesta.…”

Section: Introductionmentioning

confidence: 99%

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Determination of genetic diversity in Acacia modesta germplasm using SDS-PAGE

et al. 2024

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Biochemical markers such as protein are very important to determine genetic diversity among plant species in a given population which in turn is very important for breeders and farmers as they can then easily select the most appropriate variety to grow in a given locality. In this connection, the present study is aimed to evaluate genetic diversity in Acacia modesta germplasm through Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) technique. About 40 genotypes were subjected to SDS-PAGE analysis where a total of 12 polypeptide bands were observed in electrophoretogram. Out of which 16.67% were monomorphic while the remaining 83.33% were polymorphic. Variation found in B-2, 4, 5, 6, 7, 8, 9, 10, 11 and 12, were 20, 22.50, 32.50, 10, 2.50, 22.50, 15, 5, 2.50 and 75% respectively. Locus contribution toward genetic disagreement was 83.33%. Cluster analysis sorted all the genotypes into 9 clusters. The genotypes in one cluster were identical regarding protein profiling and showed less intra-specific genetic variation whereas differences were find from other genotypes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of genetic diversity in Acacia modesta germplasm using SDS-PAGE

et al. 2024

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“…Morpho-biochemical methods are used to screened best genotypes in large collected germplasm of different crop species [23][24][25][26][27][28][29][30]. Among these methods the SDS-PAGE method is effectively used to determinate the taxonomic and evolutionary difficulties of certain plant species [28].…”

Section: Introductionmentioning

confidence: 99%

Ethno-Pharmacognosy and Diversity Encourage Conservation of Wild Ziziphus species Collected from KP, Pakistan

Ali

2019

ARTOAJ

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The current research works to show that the medicinal practices of two different wild Ziziphus species (Ziziphus oxyphylla Edgew and Ziziphus mauritina Lamk), to assess the consensus factor between local communities of different areas of KP, to measure the potential for novel drugs of herbal origin. There was immeasurable promise among the informers concerning medicinal uses of plants with Informants Consensus Factor (ICF) value ranges from 0.992 to 0.124 with an average value as 0.440. The study naked that most of the informants agreed in the application of Z. oxyphylla to use and against Jaundice (ICF 0.992) that unveiled the highest fidelity level (100%) respectively. The present work has tried to genetic relationships among of Ziziphus species growing in Swat Valley, KP, Pakistan. The intra and inter genetic diversity among the 50 genotypes of Ziziphus species viz., 25 genotypes of Z. oxyphylla, 25 of Z. mauritina, were tested using morphometric and biochemical profiling. Twenty-four morphological characters were counted for the assessment of intra and inter genetic diversity through traits similarity index and cluster analysis. Total seed protein profiling was carried out on 12% slab gel electrophoresis; 12 reproducible bands were noticed in Z. muritiana and 8 Z. oxyphylla. Intra locus contribution toward the genetic disagreement was 41.666% in Z. oxyphylla and 25% in Z. muritiana. In the same way, inter species locus contribution toward genetic diversity was 66.666%. Fascinatingly, locus L-1, 6, 7, 8 was monomorphic in the collected germplasms and may be presented as generic specific locus for Ziziphus species.

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“…Morpho-biochemical methods are used to screened best genotypes in large collected germplasm of different crop species (BATOS et al, 2017;HASANOVA et al, 2017;PETROVIC et al, 2017;SAROEI et al, 2017;YADEGARI et al, 2017;JAN et al, 2017 b ;JAN et al, 2016 a-c ;IZADPANAH et al, 2015;ARIF et al, 2015). Among these methods the SDS-PAGE method is effectively used to determinate the taxonomic and evolutionary difficulties of certain plant species ).…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic variability in Trigonella foenum-graecum L. germplasm by SDS-page analysis

et al. 2017

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The 96 genotypes of Trigonella foenum-graecum L. (Fenugreek) were estimated by using Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE). The accessions were maintained from diverse ecological areas of the world. Total seed storage proteins were electrophoresis on 12.5% polyacrylamide gels. A total of 17 protein bands were detected, of which seven were highly polymorphic and six were moderate polymorphic and four were low polymorphic with molecular weight extending from of ~15 to ~180 kDa. The dendrogram based on similarity matrix using Unweighted Pair Group Method with Arithmetic Mean (UPGMA) divided all the genotypes into four major clusters i.e., I, II, III and IV comprising 51, 24, 10 and 11 accessions, respectively. Although limited genetic diversity was detected amongst known germplasm but the presence/absence of polymorphic bands revealed a significant variances among different Trigonella genotypes. The differences exposed in this project work should be oppressed for the future breeding prospective of Trigonella genotypes by using other advanced molecular techniques. The SDS-PAGE per se seems inadequate tool for such kind of analysis, and may be integrated with other genetic and sequence based approaches for more precise estimation of the genetic variability within closely related accessions. Our results provide baseline for obtaining locally adapted and improved cultivars of fenugreek in Pakistan.

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Study of multivariate analysis of quantitative traits in Iranian pumpkin lines

Cited by 5 publications

References 12 publications

Determination of genetic diversity in Acacia modesta germplasm using SDS-PAGE

Determination of genetic diversity in Acacia modesta germplasm using SDS-PAGE

Ethno-Pharmacognosy and Diversity Encourage Conservation of Wild Ziziphus species Collected from KP, Pakistan

Assessment of genetic variability in Trigonella foenum-graecum L. germplasm by SDS-page analysis

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