Study of morphometric parameters of podocyte foot processes in impaired calcium balance

Titova, V. A.; Pavlenko, I. N.; Balakina, A. Yu.; Barabanova, V. V.; Zueva, S. A.

doi:10.1007/bf02433368

Cited by 3 publications

(1 citation statement)

References 3 publications

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“…What is novel is the finding that the majority of the long processes (Ͼ10% of cell diameter) observed in cultured podocytes are a result of partial retraction of the lamellipodia, leaving behind thin processes where the leading edge of the lamellipodium remained attached to the substrate. Since the mean maximum diameter of podocyte foot processes is ϳ150 nm (43), it is likely that the ϳ5-m-wide cell processes observed in cultured podocytes were formed by a different process, although MS cells formed Ͻ1-m-diameter retraction spikes. These results lead to the conclusion that studies of process formation in cultured podocytes are analyses of lamellipodia formation, retraction, and the detachment of cell-substrate contacts.…”

Section: Discussionmentioning

confidence: 98%

Marker expression, behaviors, and responses vary in different lines of conditionally immortalized cultured podocytes

Chittiprol

Chen

Petrovic-Djergovic

et al. 2011

American Journal of Physiology-Renal Physiology

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The state-of-the-art cultured podocyte is conditionally immortalized by expression of a temperature-sensitive mutant of the SV40 large-T antigen. These cultures proliferate at 33°C and differentiate at 37°C into arborized cells that more closely resemble in vivo podocytes. However, the degree of resemblance remains controversial. In this study, several parameters were measured in podocyte cell lines derived from mouse (JR, KE), human (MS), and rat (HK). In all lines, the quantities of NEPH1 and podocin proteins and NEPH1 and SYNPO mRNAs were comparable to glomeruli, while synaptopodin and nephrin proteins and NPHS1 and NPHS2 mRNAs were <5% of glomerular levels. Expression of Wilms' tumor-1 (WT1) mRNA in mouse lines was comparable to glomeruli, but rat and human lines expressed little WT1. Undifferentiated human and mouse lines had similar proliferation rates that decreased after differentiation, while the rate in rat cells remained constant. The motility of different lines varied as measured by both general motility and wound-healing assays. The toxicity of puromycin aminonucleoside was MS ∼ JR >> KE, and of doxorubicin was JR ∼ KE > MS, while HK cells were almost unaffected. Process formation was largely a result of contractile action after formation of lamellipodia. These findings demonstrate dramatic differences in marker expression, response to toxins, and motility between lines of podocytes from different species and even between similarly-derived mouse lines.

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Section: Discussionmentioning

confidence: 98%