Study of Microbial Transglutaminase Partitioning in Thermo-pH–Responsive Aqueous Two-Phase Systems

Wang, Zhenping; Chen, Xi; Wan, Jiamin; Cao, Xuejun

doi:10.1007/s12010-020-03394-8

Cited by 10 publications

(5 citation statements)

References 29 publications

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“…The PN could be easy to precipitate from solution above its LCST. It was mainly due to that the macromolecular chain of PN has both hydrophilic amido groups and hydrophobic isopropyl groups [27], which makes PN aqueous solutions exhibit temperature sensitive characteristics. When the temperature rised, the polymer molecular chain and the water separated leading to the precipitation of the polymer.…”

Section: Recovery Of P N and P Adb478mentioning

confidence: 99%

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Partition of Tea Saponin with a Novel Recyclable Thermo-pH Aqueous Two-Phase Systems

Wei

Chen

Yang

et al. 2021

Appl Biochem Biotechnol

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Aqueous two-phase systems (ATPS) have the advantages of environmentally friendly, high mass transfer efficiency, mild extraction conditions. However, it is difficult to recycle these components, which limits the large-scale application of ATPS in industrial production. In this study, a novel recyclable ATPS of PN/PADB4.78 was constructed with a thermo-responsive polymer PN and a pH-responsive polymer PADB4.78 for the partition study of tea saponin. The recovery of PN reached 98.71% by adjusting the temperature to above its lower critical solution temperature (LCST, 33.0 °C ) and the recovery of PADB4.78 was 97.32% by regulating the pH to its isoelectric point (pI, 4.78). Meanwhile, the phase formation mechanism of ATPS was studied by surface tension and low-field nuclear magnetic resonance (LF-NMR). The effects of polymer concentration, pH, temperature, types and concentrations of salt were investigated on tea saponin partition.In the 1.5% PN/3.5% PADB4.78 ATPS, the optimal partition coefficient (K) and extraction recovery (ERb) of crude tea saponin were 0.15 and 92.13% in the presence of 1.5 mM KCl at pH=7.6 and 25℃, respectively. The K and ERb of tea saponin from tea seeds were 0.12 and 94.50% with 7.5 mM LiBr at pH=8.0 and 25℃, respectively.

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Section: Recovery Of P N and P Adb478mentioning

confidence: 99%

“…Before the phase formation,the surface tension of the two polymers were quite different. It was precisely that two polymer solutions can quickly form aqueous two-phase system after mixing because of the difference in surface tension[27].…”

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confidence: 99%

Partition of Tea Saponin with a Novel Recyclable Thermo-pH Aqueous Two-Phase Systems

Wei

Chen

Yang

et al. 2021

Appl Biochem Biotechnol

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“…The mechanism was that sodium ions and chloride ions had a concentration difference between the two phases, resulting in a potential difference. The partition coefficient K of a biomolecule varies exponentially with the electrochemical potential difference between the phases and the net charge of the partitioned biomolecule (Table 1) [26][27][28]. 1A shows the PEG/phosphate system (formulation 2) with no significant difference between the upper and lower phases.…”

Section: Investigation On Aqueous Two-phase Systems and Protein Drugsmentioning

confidence: 99%

Self-Encapsulation of Biomacromolecule Drugs in Porous Microscaffolds with Aqueous Two-Phase Systems

Kang

Cai

et al. 2021

Pharmaceutics

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At present, the most commonly used methods of microencapsulation of protein drugs such as spray drying, multiple emulsification, and phase separation, can easily cause the problem of protein instability, which leads to low bioavailability and uncontrolled release of protein drugs. Herein, a novel method to encapsulate protein drugs into porous microscaffolds effectively and stably was described. Ammonium hydrogen carbonate (NH4HCO3) was employed to prepare porous microscaffolds. α-Amylase was encapsulated into the porous microscaffolds without denaturing conditions by an aqueous two-phase system (PEG/Sulfate). The pores were closed by heating above the glass transition temperature to achieve a sustained release of microscaffolds. The pore-closed microscaffolds were characterized and released in vitro. The integrity and activity of protein drugs were investigated to verify that this method was friendly to protein drugs. Results showed that the pores were successfully closed and a high loading amount of 9.67 ± 6.28% (w/w) was achieved. The pore-closed microscaffolds released more than two weeks without initial burst, and a high relative activity (92% compared with native one) of protein demonstrated the feasibility of this method for protein drug encapsulation and delivery.

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“…Double-stimuli-responsive systems provide a higher degree of customization of the target application and thus can be more easily adapted to industrial applications. Combining a thermo-sensitive polymer and a pH-sensitive polymer as the phase-forming agents is the most pursued route to develop double-stimuli-responsive ABS. − To the best of our knowledge, the development of IL-based ABS with double responsiveness to temperature and pH was not previously reported.…”

Section: Introductionmentioning

confidence: 99%

Double-Stimuli-Responsive (Temperature and pH) Aqueous Biphasic Systems Comprising Ionic Liquids

Rufino

Bola

Capela

et al. 2023

ACS Sustainable Chem. Eng.

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Aqueous biphasic systems (ABS) can integrate multiple unit operations and operate under continuous mode, contributing to the development of sustainable separation processes. Encouraged by the designer solvent features of ionic liquids (ILs), we herein propose their use as components of double-stimuli-responsive (temperature- and pH-driven) ABS. Resorting to choline-alkanoate-based ILs as the pH-responsive components and poly(propylene glycol) (PPG 400) as the thermo-responsive component, the ABS ternary phase diagrams are determined at various temperature (25–45 °C) and pH (3–7) conditions. While the liquid–liquid phase diagram response to temperature obeys a lower critical solution temperature-like behavior, the response to pH correlates with the pK a of the IL anion parent acid. The simultaneous responsiveness to temperature and pH is then shown, whose results inspire the development of customizable separation techniques as proved with the simultaneous (one-step) separation of two dyes. By a proper customization of the IL chemical structure and stimuli applied, ABS may be designed to improve the performance and sustainability of separation processes.

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Study of Microbial Transglutaminase Partitioning in Thermo-pH–Responsive Aqueous Two-Phase Systems

Cited by 10 publications

References 29 publications

Partition of Tea Saponin with a Novel Recyclable Thermo-pH Aqueous Two-Phase Systems

Partition of Tea Saponin with a Novel Recyclable Thermo-pH Aqueous Two-Phase Systems

Self-Encapsulation of Biomacromolecule Drugs in Porous Microscaffolds with Aqueous Two-Phase Systems

Double-Stimuli-Responsive (Temperature and pH) Aqueous Biphasic Systems Comprising Ionic Liquids

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