Study of methylation reactions of 2-phenylquinazoline-4-tion with “soft” and “hard” methylation agents and determination of its biological activity

Abstract

Alkylation reactions of 2-phenylquinazoline-4-thion with methylation agents “soft” (methyl iodide) and “hard” (dimethyl sulfate, methyltozylate) were studied. It was found that the reaction proceeds with the formation of alkyl products at the N3 - and S4 - reaction centers, depending on the methylation agent, solvent and temperature. This indicated the ambivalent nature of the 2-phenylquinazoline-4-tion anion. Prolongation of the reaction time leaded to the formation of a second isomeric product (VII). A slight increase in phenyl N3-product (VII) yield was noted when dimethyl sulfate and methylfolate were used as methylation agents. In non-polar proton-free solvent DMF and dipolar proton-free solvent acetonitrile, only N-methyl product (VII) was formed because of the reaction. An increase in the polarity of the solvent and the “hardness” of the methylation agent leads to an increase in the yield of N3 products.

Chromato-mass-spectrometry of the analysis of the sum of the common mushrooms

“…The process involves introducing the "seeds" into the substrate, where the mycelium will grow. Properly prepared compost should exhibit slight resilience when pressed [1][2][3]. Depressions, spaced 20 centimeters apart, are made at a depth of 5 centimeters and filled with a small amount of compost mycelium.…”

“…In order to obtain an extract for analysis, a representative sample of the raw material was first crushed to a specific particle size. The crushed material was then placed in a filter paper and loaded into the Soxhlet apparatus for extraction, which was carried out using 100% alcohol for a duration of 2.5 hours in a boiling water bath [1][2][3][4][9][10][11]. The resulting alcoholic extract was subsequently concentrated on a rotary evaporator, and the resulting concentrate was then quantitatively transferred to a volumetric flask, where the volume was adjusted using the same alcohol to reach the mark.…”

“…The solution was thoroughly mixed prior to analysis. Spectral data was collected using a "chromatek crystal" spectrophotometer equipped with a mass spectrometric detector, which was produced in Russia in the year 2022 [1][2][3][4][5]9].…”

“…It thrives in various regions spanning Europe, Africa, Asia, and the Americas. The name champignon, derived from the French language, translates to mushroom in English [1][2][3][4][5][6][7].…”

“…Agaricus, also known as champignon, belongs to the Agaricaceae family within the Agaricales order. The fruit bodies of this species vary in size, ranging from 3 cm (Agaricus comtulus) to 25 cm (Agaricus arvensis) [1][2][3]12].…”