Study of method and system for diagnosis of cancer using autofluorescence and Raman spectroscopy

Abstract

induced fluorescence(LIF) and Raman spectra were measured from normal and tumorous human serum in an attempt to discover some values useful in discrimination between normal and tumorous cases. Red shift of fluorescence peak and decrease of fluorescence intensity were observed after samples radiated by laser. According to one thousand twenty-two samples' spectra, three parameters α, β and δγ are introduced to distinguish normal, benign and malignant from one another. The application of such parameters in clinical diagnosis was researched. The practical instrument of laser-induced serum fluorescence and resonance Raman spectra for cancer diagnosis or incipient cancer is designed, by combining laser spectroscopy, biomedical, photo-electron technology, controlling technology and computer technology. The instrument is intelligent for operating and diagnosing. The clinic application of this instrument has been carried out successfully in the diagnosing of stomach cancer and liver cancer (459 cases); the accuracy is about 85%. It develops a new technology in the field of cancer diagnosis.

Simple and robust diagnosis of early, small and AFP-negative primary hepatic carcinomas: an integrative approach of serum fluorescence and conventional blood tests

“…In this study, we developed a novel method by which to simultaneously measure the serum autofluorescence and cfDNA-related fluorescence using a real-time PCR system as a convenient fluorometer with temperature and time control “functions”. Compared with the conventional method for detecting serum autofluorescence, which relies on the use of a spectrofluorometer [ 10 – 12 ], our method is rapid, high-throughput and efficient. All operations could be completed within half an hour.…”

“…The serum autofluorescence intensity reportedly gradually increased as liver fibrosis progressed in rats [ 24 ]. Changes in the serum autofluorescence have been exploited for the diagnosis of chronic liver diseases [ 10 – 12 ]. Additionally, It was reported that indirect bilirubin was more fluorescent than direct bilirubin in bile [ 25 ], but we did not found this phenomenon in serum as the correlation coefficients of indirect bilirubin were similar with direct bilirubin ( Table S2 ).…”

“…A few reports have shown that the serum autofluorescence produced by native fluorescent substances in the blood may be used to diagnose liver cancer [ 10 – 12 ]. The laser-induced serum Raman spectra of patients with liver cancer differed from those of cirrhosis patients or normal subjects [ 11 ], and this difference was diagnostically significant for liver cancer [ 10 ]. However, the principle component of the spectra could not discriminate cirrhosis patients with and without HCC [ 12 ].…”

Combined Raman and autofluorescence ex vivo diagnostics of skin cancer in near-infrared and visible regions

“…This effect may be partly compensated (approximately by 5% to 10%) in a multimodal approach, 21 when each modality uses optical parameters associated with different tissue components altering during the cancerous process. The combination of RS and AF has been demonstrated for gastric, 21 lung, 22,23 liver, 24 and breast tissues model 25 experiments. Yet the exact RS-AF diagnostic accuracy is still unclear for different skin tumor types.…”

Combination of dual serum fluorescence, AFP and hepatic function tests is valuable to identify HCC in AFP-elevated liver diseases

“…Serum-based detection is simple, practical, easily repeated and minimally invasive. Serum autofluorescence is a potential biomarker for liver diseases [ 10 ], with an accuracy of 85% for diagnosing liver cancer [ 11 ]. Cell-free circulating DNA (cfDNA) in serum has been reported as a new tumor marker based on increases in various malignancies, including HCC [ 12 ].…”