Study of malondialdehyde as an oxidative stress marker in schizophrenia

Dahake, Hemant; Warade, Jayesh; Kansara, Gaurav Shashikant; Pawade, Yogesh; Ghangle, Suresh

doi:10.18203/2320-6012.ijrms20163759

Cited by 6 publications

(5 citation statements)

References 13 publications

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“…Furthermore, GSH, GSH-Px and SOD decrease the levels of ROS to prevent the oxidation of proteins, lipids and DNA ( 39 ). MDA is an end-product of ROS-induced peroxidation, and it is commonly used as an oxidative stress biomarker ( 40 ). 8-OH-dG, a product of DNA oxidation, is a marker of oxidative DNA damage ( 41 ).…”

Section: Discussionmentioning

confidence: 99%

Ginsenoside Rg1 attenuates liver injury induced by D‑galactose in mice

et al. 2018

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The present study investigated the effect and underlying mechanisms of ginsenoside Rg1 (Rg1) in attenuating subacute liver injury induced by D-galactose (D-gal) in mice. Specific Pathogen Free (SPF) male C57BL/6J mice were randomly divided into 3 groups: i) D-gal-administration group (D-gal group), where the mice were intraperitoneally administrated with D-gal (120 mg/kg/day for 42 days); ii) D-gal + Rg1 group where the mice were treated with 120 mg/kg/day D-gal for 42 days and with Rg1 at a dose of 20 mg/kg/day for 35 days. The first dose of Rg1 was administered on the 8th day of treatment with D-gal; and iii) the normal control group, where the mice were injected with an equal volume of saline for 42 days. The day following the final injections in all groups, peripheral blood was collected and serum was prepared to measure the contents of aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (TBiL), advanced glycation end products (AGEs) and 8-hydroxy-2 deoxyguanosine (8-OH-dG). Liver tissue homogenates were prepared to measure the contents of malondialdehyde (MDA) and glutathione (GSH), and the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD). Paraffin section were prepared to observe the microscopic structure of the liver. Transmission electron microscopy was used to observe the ultrastructure of hepatocytes. Frozen section were prepared and stained with senescence-associated β-galactosidase to detect the relative optical density value of senescence-associated markers. Compared with the D-gal group, the contents of AST, ALT, TBiL, AGEs and MDA significantly decreased in the D-gal + Rg1 group, while the activities of SOD and GSH-Px markedly increased, and liver injury and degenerative alterations of hepatocytes were reduced. Administration of Rg1 induced a protective effect on D-gal-induced liver injury in mice by inhibiting the oxidative stress, reducing DNA damage and decreasing the AGE content.

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Section: Discussionmentioning

confidence: 99%

Ginsenoside Rg1 attenuates liver injury induced by D‑galactose in mice

et al. 2018

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“…In general, MDA is suggested as the efficient stress-marker for animals, especially mammals (Nielsen et al 1997). Being one of the final products of polyunsaturated fatty acids peroxidation in the cells, its formation indicates the generation of free radicals in tissues (Gawel et al 2004;Dahake et al 2016). As mentioned earlier, the results shown by MDA were correlated to the results of stress indicators (SOD, catalase and glutathione).…”

Section: Acidic Ph Affects Zebrafish Skeletal Musclementioning

confidence: 91%

Acidic ambiance induced post-oxidative stress aﬀects AMPK- PGC1α-SIRT1 axis in the skeletal muscles of zebraﬁsh Danio rerio Hamilton, 1822

et al. 2021

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The present study was aimed to understand whether acidic pH induces oxidative stress in zebrafish affecting metabolic sensor protein and thereby, the mitochondrial functions in the skeletal muscle of zebrafish. The experiments performed in aquaria involved the study of the changes of HIF1α, AMPK, PGC1α and SIRT1 levels together with the levels of mitochondrial Tfam and Nrf1. The results obtained from investigation of superoxide dismutase (SOD), catalase and glutathione revealed that the fish undergoes oxidative stress within a short duration of exposure to acidic ambiance. Further analysis with MDA and HIF1α helped to understand the effects of post oxidative stress on skeletal muscle of the fish at pH 5.0 (± 0.5). Of the three tissues studied (gill, brain and skeletal muscle) the effect was maximum in skeletal muscle as depicted by MDA level at 2 hours beyond which it declines augmenting death or mortality (15%) to the fish. Consequently, HIF1α was increased as an adaptive strategy against metabolic disruption during the first 2 hours period. However, on exposure to pH 5.0 (± 0.5) for 2 hours, there were decrease of the metabolic sensors viz. AMPK and SIRT1 followed by mitochondrial gene transcriptional co-activator PGC1α. The expression of mitochon-drial transcription factors Tfam and Nrf1 were also reduced confirming perturbation in mitochondrial function affecting low ATP production compared to control. This was also supported by the decrease of COXII as well as mitochondrial complex I activity. All these results confirm that the metabolic machinery of zebrafish is affected when pH was lowered to 5.0 (± 0.5).

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“…However, an interesting study conducted on schizophrenia patients drug-free has reported reduced plasma GSH and total thiol but significantly increased MDA and GPx [70]. Another one using first-episode schizophrenia patients and chronic schizophrenia patients reported significantly higher plasma MDA levels [71], whereas another study noted that in the case of first-episode psychosis, the levels of SOD and CAT were 40% lower, reduction that can be ameliorated by antipsychotic administration [72]. Elevated MDA levels are confirmed by other studies as well [73], alongside NO [74][75][76][77] and decreased levels of GSH, SOD [74,75,77,78], reduced catalase, GPx, glutathione reductase, total antioxidant status, and lower peroxidation [75,76,79,80].…”

Section: Schizophrenia and Diagnosticmentioning

confidence: 99%

Potential Novel Therapies for Neurodevelopmental Diseases Targeting Oxidative Stress

Curpăn

Luca

Ciobîcă

2021

Oxidative Medicine and Cellular Longevity

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Neurodevelopmental disorders are a category of diseases that is not yet fully understood. Due to their common traits and pathways, often it is difficult to differentiate between them based on their symptoms only. A series of hypotheses are trying to define their etiology, such as neuroinflammation, neurodegeneration, and immunology, but none have managed to explain their multifactorial manifestation. One feature that may link all theories is that of oxidative stress, with a redox imbalance as well as several other markers of oxidative damage (on lipids, proteins, and nucleic acids) being observed in both postmortem samples of the brain of patients with schizophrenia and autism spectrum disorders. However, the implication of oxidative stress in pathology is still distrustfully looked upon. For this purpose, in the current paper, we were interested in reviewing the implications of oxidative stress in these disorders as well as the impact of N-acetylcysteine on the oxidative status with a focus on the glutathione level and N-methyl-D-aspartate receptor. We were also interested in finding papers targeting the use of antioxidant properties of different plant extracts.

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Study of malondialdehyde as an oxidative stress marker in schizophrenia

Cited by 6 publications

References 13 publications

Ginsenoside Rg1 attenuates liver injury induced by D‑galactose in mice

Ginsenoside Rg1 attenuates liver injury induced by D‑galactose in mice

Acidic ambiance induced post-oxidative stress aﬀects AMPK- PGC1α-SIRT1 axis in the skeletal muscles of zebraﬁsh Danio rerio Hamilton, 1822

Potential Novel Therapies for Neurodevelopmental Diseases Targeting Oxidative Stress

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