Study of magnetic particles pulse-injected into an annular SPLITT-like channel inside a quadrupole magnetic field

Hoyos, Mauricio; Moore, Lee R.; McCloskey, Kara E.; Margel, Shlomo; Zuberi, Merav; Chalmers, Jeffrey J.; Zborowski, Maciej

doi:10.1016/s0021-9673(00)00879-7

Cited by 45 publications

(72 citation statements)

References 15 publications

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“…The effects of flow rates on the QMS performance have been theoretically analyzed and experimentally investigated in previous studies (Hoyos et al, 2000(Hoyos et al, , 2002Nakamura et al, 2001;Williams et al, 1999). This manuscript focuses on the utilization of outlet flow rate control to collect the CD34þ cells with different purity and recovery.…”

Section: Introductionmentioning

confidence: 99%

Blood progenitor cell separation from clinical leukapheresis product by magnetic nanoparticle binding and magnetophoresis

Jing

Moore

Williams

et al. 2006

Biotech & Bioengineering

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Positive selection of CD34+ blood progenitor cells from circulation has been reported to improve patient recovery in applications of autologous transplantation. Current magnetic separation methods rely on cell capture and release on solid supports rather than sorting from flowing suspensions, which limits the range of therapeutic applications and the process scale up. We tested CD34+ cell immunomagnetic labeling and isolation from fresh leukocyte fraction of peripheral blood (leukapheresis) using the continuous quadrupole magnetic flow sorter (QMS), consisting of a flow channel (SHOT, Greenville, IN) and a quadrupole magnet with a maximum field intensity (B(o)) of 1.42 T and a mean force field strength (S(m)) of 1.45 x 10(8) TA/m(2). Both the sample magnetophoretic mobility (m) and the inlet and outlet flow patterns highly affect the QMS performance. Seven commercial progenitor cell labeling reagent combinations were quantitatively evaluated by measuring magnetophoretic mobility of a high CD34 expression cell line, KG-1a, using the cell tracking velocimeter (CTV). The CD34 Progenitor Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) showed the strongest labeling of KG-1a cells and was selected for progenitor cell enrichment from 11 fresh and 11 cryopreserved clinical leukapheresis samples derived from different donors. The CD34+ cells were isolated with a purity of 60-96%, a recovery of 18-60%, an enrichment rate of 12-169, and a throughput of (1.7-9.3) x 10(4) cells/s. The results also showed a highly regular dependence of the QMS performance on the flow conditions that agreed with the theoretical predictions based on the CD34+ cell magnetophoretic mobility.

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Section: Introductionmentioning

confidence: 99%

Blood progenitor cell separation from clinical leukapheresis product by magnetic nanoparticle binding and magnetophoresis

Jing

Moore

Williams

et al. 2006

Biotech & Bioengineering

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“…[41][42][43][44] Their versatility has been demonstrated by use in conjunction with techniques such as the polymerase chain reaction, 45 mass spectroscopy, 46 and high-throughput linear magnetophoresis (LM) assays. 41,[47][48][49][50] Precise application of magnetic fields has enabled device-based quadrupole sorting, [51][52][53][54] active bead transport for biosensing, 55 and dynamic manipulation of magnetic supraparticle structures (SPSs). 56 For applications in which target analytes contain multiple binding sites or epitopes, it is possible for the target to bind to multiple beads simultaneously, leading to aggregation.…”

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confidence: 99%

Resistive pulse sensing of magnetic beads and supraparticle structures using tunable pores

Willmott

Platt

2012

Biomicrofluidics

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Tunable pores (TPs) have been used for resistive pulse sensing of 1 lm superparamagnetic beads, both dispersed and within a magnetic field. Upon application of this field, magnetic supraparticle structures (SPSs) were observed. Onset of aggregation was most effectively indicated by an increase in the mean event magnitude, with data collected using an automated thresholding method. Simulations enabled discrimination between resistive pulses caused by dimers and individual particles. Distinct but time-correlated peaks were often observed, suggesting that SPSs became separated in pressure-driven flow focused at the pore constriction. The distinct properties of magnetophoretic and pressure-driven transport mechanisms can explain variations in the event rate when particles move through an asymmetric pore in either direction, with or without a magnetic field applied. Use of TPs for resistive pulse sensing holds potential for efficient, versatile analysis and measurement of nano-and microparticles, while magnetic beads and particle aggregation play important roles in many prospective biosensing applications.

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“…Based on these trajectories and the total flow-rate, the final position of each particle is calculated. Both further details of the code and experimental evaluation of its prediction for cell and particle suspensions have been previously published (Hoyos et al, 2000;McCloskey et al, 2003a). In terms of operating the QMS in a deposition mode, the system has yet to be modeled; nevertheless, the operation is conceptually much simpler than the completely continuous mode in that the flow rate of the cell suspension in equals the flow rate of the cell suspension out.…”

Section: Cell Sorting With Quadrupole Magnetic Cell Sortermentioning

confidence: 99%

Comparison of two immunomagnetic separation technologies to deplete T cells from human blood samples

Lara

Tong

Zborowski

et al. 2006

Biotech & Bioengineering

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The objective of this study was to compare the performance of two immunomagnetic separation technologies to deplete T cells from buffy coats of human blood. Specifically, two versions of the commercial MACS(R) Technology: MiniMACS and SuperMACS, and a prototype, flow-through system, the QMS, were evaluated. Peripheral blood mononuclear leukocytes (PBL) were isolated from buffy coats and an immunomagnetic separation of CD3(+) cells was conducted using company and optimized labeling protocols. To mimic peripheral blood containing bone marrow purged hematopoietic stem cells, HSC, CD34 expressing-cells (KG1a) were spiked into PBL prior to T-cell depletion once optimized depletion conditions were determined. Once the labeling protocol was optimized, the MiniMACS system performed well by producing a highly enriched CD3(+) fraction, and a respectable level of depletion of T cells and recovery of KG1a cells in the depleted fraction; an average log(10) depletion of T cells of 2.88 +/- 0.17 and an average recovery of the KG1a cells of 60.8 +/- 5.94% (n = 14). The performance of the SuperMACS system was very similar with an average log(10) depletion of T cells of 2.89 +/- 0.22 and an average recovery of KG1a of 63.1 +/- 8.55% (n = 10). In contrast, the QMS system produced an average log(10) depletion of T cells of 3.98 +/- 0.33 (n = 16) with a corresponding average recovery of 57.9 +/- 16.6% of the spiked CD34+ cells. The aforementioned QMS performance values were obtained using sorting speeds ranging from 2.5 x 10(4) to 1.7 x 10(5) cells per second. It is suggested that the lack of a 100% recovery of the unlabeled KG1a cells is the result of a previously reported "drafting" phenomena which pulls unlabeled cells in the direction of the magnetically labeled cells thereby resulting in loss of the unlabeled cells.

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Study of magnetic particles pulse-injected into an annular SPLITT-like channel inside a quadrupole magnetic field

Cited by 45 publications

References 15 publications

Blood progenitor cell separation from clinical leukapheresis product by magnetic nanoparticle binding and magnetophoresis

Blood progenitor cell separation from clinical leukapheresis product by magnetic nanoparticle binding and magnetophoresis

Resistive pulse sensing of magnetic beads and supraparticle structures using tunable pores

Comparison of two immunomagnetic separation technologies to deplete T cells from human blood samples

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