Study of lysophosphatidic acid receptors (LPARs) in buffalo uterus demonstrated upregulation of LPAR1 and LPAR6 in early pregnancy

Sadam, Abdul; Parida, Subhashree; Padol, Amol R; Verma, Ankita; Baba, Naseer Ahmad; Khuman, Wanta M.; Srivastava, Vivek; Panigrahi, Manjit; Singh, Thakur Uttam; Sarkar, Srobana

doi:10.1016/j.theriogenology.2017.05.019

Cited by 8 publications

(5 citation statements)

References 30 publications

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“…We also detected the DGKG gene, which affects insulin signalling and lipid metabolism, and is a regulator of diacylglycerol and phosphatidic acid, which are important mediators of signal transduction (Sinha et al., 2020). We detected several candidate genes through the xp‐EHH analysis that were previously reported in other livestock, like PITX1 and KHDRBS1 in goats (Ma et al., 2017; Sayre, 2010); LPAR6 in buffaloes (Sadam et al., 2017); RTL1 in pigs (Yang et al., 2009); LRRC18 and CLDN17 in horses (Al Abri et al., 2018); and CLDN8, CLDN17 and MATN1 in cattle (Araujo et al., 2009; Campos et al., 2017; Kijas et al., 2006).…”

Section: Discussionmentioning

confidence: 75%

A selection signatures study among Middle Eastern and European sheep breeds

Eydivandi

Roudbar

Ardestani

et al. 2021

J Animal Breeding Genetics

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Selection, both natural and artificial, leaves patterns on the genome during domestication of animals and leads to changes in allele frequencies among populations. Detecting genomic regions influenced by selection in livestock may assist in understanding the processes involved in genome evolution and discovering genomic regions related to traits of economic and ecological interests. In the current study, genetic diversity analyses were conducted on 34,206 quality‐filtered SNP positions from 450 individuals in 15 sheep breeds, including six indigenous breeds from the Middle East, namely Iranian Balouchi, Afshari, Moghani, Qezel, Karakas and Norduz, and nine breeds from Europe, namely East Friesian Sheep, Ile de France, Mourerous, Romane, Swiss Mirror, Spaelsau, Suffolk, Comisana and Engadine Red Sheep. The SNP genotype data generated by the Illumina OvineSNP50 Genotyping BeadChip array were used in this analysis. We applied two complementary statistical analyses, FST (fixation index) and xp‐EHH (cross‐population extended haplotype homozygosity), to detect selection signatures in Middle Eastern and European sheep populations. FST and xp‐EHH detected 629 and 256 genes indicating signatures of selection, respectively. Genomic regions identified using FST and xp‐EHH contained the CIDEA, HHATL, MGST1, FADS1, RTL1 and DGKG genes, which were reported earlier to influence a number of economic traits. Both FST and xp‐EHH approaches identified 60 shared genes as the signatures of selection, including four candidate genes (NT5E, ADA2, C8A and C8B) that were enriched for two significant Gene Ontology (GO) terms associated with the adenosine metabolic procedure. Knowledge about the candidate genomic regions under selective pressure in sheep breeds may facilitate identification of the underlying genes and enhance our understanding on these genes role in local adaptation.

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Section: Discussionmentioning

confidence: 75%

A selection signatures study among Middle Eastern and European sheep breeds

Eydivandi

Roudbar

Ardestani

et al. 2021

J Animal Breeding Genetics

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“…Membrane proteins (detergent‐soluble hydrophobic membrane proteins) were extracted according to the manufacturer's instructions of the Membrane Protein Extraction Kit (Boster Bio, Pleasanton, CA, USA) as described previously (Sadam et al., ). Lipid raft proteins were isolated using a lipid raft isolation kit (Sigma‐Aldrich, St. Louis, MO, USA), following the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Casein kinase 2 inhibition impairs spontaneous and oxytocin‐induced contractions in late pregnant mouse uterus

Suhas

Parida

Gokul

et al. 2018

Experimental Physiology

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The protein kinase casein kinase 2 (CK2) is a ubiquitously expressed serine or threonine kinase known to phosphorylate a number of substrates. The aim of this study was to assess the effect of CK2 inhibition on spontaneous and oxytocin-induced uterine contractions in 19 day pregnant mice. The CK2 inhibitor CX-4945 elicited a concentration-dependent relaxation in late pregnant mouse uterus. CX-4945 and another selective CK2 inhibitor, apigenin, also inhibited the oxytocin-induced contractile response in late pregnant uterine tissue. Apigenin also blunted the prostaglandin F response, but CX-4945 did not. Casein kinase 2 was located in the lipid raft fractions of the cell membrane, and disruption of lipid rafts was found to reverse its effect. The results of the present study suggest that CK2, located in lipid rafts of the cell membrane, is an active regulator of spontaneous and oxytocin-induced uterine contractions in the late pregnant mouse.

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“…The amplification was carried out in 20 µl volume reaction mixture containing 10 µl (2×) master mix (Fast SYBR Green qRT‐PCR Master Mix), 1 µl (10 µM) gene‐specific forward and reverse primers, 2 µl cDNA template and 6µl nuclease‐free water and by following thermal profile of Fast SYBR Green Master Mix: 95°C for 20 s, followed by 40 cycles of 95°C for 3 s and 57–60°C for 30 s. Non‐template control was included in each run. The GAPDH gene, a housekeeping gene, was used as an endogenous control for the analysis of data as it is the most widely used (Davies et al, ; Lee et al, ; Sadam et al, ; Verma et al, ). Hence, relative expression of (i) GAPDH , as endogenous control, and (ii) LGALS1 , LGALS3BP , LGALS9 , SLC2A1 , SLC5A11 , MUC1, CTSL and CST3 genes as target genes was carried out, in both pregnancy stages as well as in the nonpregnant stage (calibrator group).…”

Section: Methodsmentioning

confidence: 99%

Endometrial transcript profile of progesterone‐regulated genes during early pregnancy of Water Buffalo (Bubalus bubalis)

Baba

Panigrahi

Verma

et al. 2018

Reprod Domestic Animals

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Progesterone (P ) plays a key role in the establishment and maintenance of pregnancy in most mammals. Unravelling the expression of progesterone-regulated genes can expand the understanding of the embryonic mortality. Accordingly, we studied the relative mRNA expression of the P -regulated genes in the buffalo. Uteri were collected from the abattoir and categorized into nonpregnant late luteal phase, stage I (28-38th days of gestation) and stage II (48-56th days of gestation) of pregnancy (n = 6/group). After extraction of total RNA from the endometrial tissues, we carried out qRT-PCR for determining the relative mRNA expression of the P -regulated genes using nonpregnant late luteal phase as calibrator group. The expression of LGALS3BP (essential for maternal recognition of pregnancy) gene was found to be significantly upregulated (p < 0.05), while MUC1 (important for embryo attachment) gene was downregulated in stage I and II of pregnancy. We observed no significant change in the expression of LGALS1, LGALS9 and CTSL genes. The SLC5A11 and SLC2A1 genes (involved in the transport of glucose to endometrium) in early pregnancy were upregulated in the pregnancy stage I (p < 0.05) relative to nonpregnant late luteal phase. The CST3 gene was significantly upregulated in pregnancy stage II (p < 0.01). These results provide molecular insights into the specific pathways involved in foeto-maternal communication during early pregnancy in buffaloes.

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Study of lysophosphatidic acid receptors (LPARs) in buffalo uterus demonstrated upregulation of LPAR1 and LPAR6 in early pregnancy

Cited by 8 publications

References 30 publications

A selection signatures study among Middle Eastern and European sheep breeds

A selection signatures study among Middle Eastern and European sheep breeds

Casein kinase 2 inhibition impairs spontaneous and oxytocin‐induced contractions in late pregnant mouse uterus

Endometrial transcript profile of progesterone‐regulated genes during early pregnancy of Water Buffalo (Bubalus bubalis)

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