Study of liver function and expression of some detoxification genes in the male rats exposed to methyl-tertiary butyl ether

Badr, Ahmad Ali; Saadat, Iraj; Saadat, Mostafa

doi:10.1016/j.ejmhg.2015.10.002

Cited by 6 publications

(7 citation statements)

References 20 publications

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“…The present data confirm previously published articles which they reported that exposure to MTBE was not associated with alteration in the GSTs gene expression levels [ 24 , 25 ]. Based on the several studies, it is suggested that MTBE may be attributed to induction of oxidative stress in the reproductive system [ 26 , 27 ].…”

Section: Resultssupporting

confidence: 93%

“…Previously we reported the mRNA levels of Gstt1 , Gstm1 , and Gstp1 in the liver of rats exposed to various concentrations of MTBE when the animals were treated for 30 days [ 25 ]. In order to neutralize the effect(s) of concentrations of MTBE and usage of peanut oil on the relationship between exposure time and mRNA levels of the examined genes, multiple linear regression analysis were used.…”

Section: Resultsmentioning

confidence: 99%

“…Real-time quantitative PCR (qPCR) was done to detect the gene expression assay of Gstm1 , Gstp1 and Gstt1 using syber master mix (Amplicon, Germany) on rotor gene 6000 detection system (Corbett life science, Germany). Quantitative real-time PCR conditions were described previously [ 25 ]. All primer pairs which used in the present study were designed by Allele ID v7.8 software and they were specific for mRNA, did not amplify genomic DNA, as described previously.…”

Section: Methodsmentioning

confidence: 99%

“…There are only two studies investigating the effect(s) of MTBE on expression levels of GSTs when rats exposed for about one month [ 24 , 25 ]. We know that in general people are exposed to MTBE for a short period.…”

Section: Introductionmentioning

confidence: 99%

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Expression Levels of Some Detoxification Genes in Liver and Testis of Rats Exposed to a Single Dose of Methyl-Tertiary Butyl Ether

Badr¹,

Saadat²

2016

Open Access Maced J Med Sci

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AIM:Methyl-tertiary-butyl ether (MTBE), a well-known gasoline oxygenate compound, is still used in several countries. Several studies investigated the effects of MTBE on the activity of phase II metabolism enzymes. There is no published data on the effect(s) of short-term exposure to MTBE on mRNA levels of antioxidant genes. Therefore, the present study was carried out.METHODS:A total of 15 adults male Wistar rats were randomly divided into five equal experimental groups. They received a single dose of 0, 400, 800 and 1600 mg/Kg MTBE in peanut oil by gavages. The final group received no MTBE and peanut oil. After 24 hr animals were slaughtered then livers and testis were removed to extract the total RNA. Real-time PCR was done to detect the gene expressions of glutathione S-transferase family (Gstt1, Gstm1, and Gstp1).RESULTS:The mRNAs levels of the examined genes neither in liver nor in testis showed a significant difference between the exposed groups and control rats.CONCLUSIONS:The present data revealed that exposure to a single dose of MTBE has no significant effect on the mRNA levels of the Gstt1, Gstm1, and Gstp1 genes.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression Levels of Some Detoxification Genes in Liver and Testis of Rats Exposed to a Single Dose of Methyl-Tertiary Butyl Ether

Badr¹,

Saadat²

2016

Open Access Maced J Med Sci

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“…The encoded protein may play an antioxidant protective role in cells and may contribute to the antiviral activity of CD8 (+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression [17]. Studying the effect of MTBE inhalation on rats might provide evidence for cancer signs in humans.…”

Section: Introductionmentioning

confidence: 99%

Impact Effect of Methyl Tertiary-Butyl Ether “Twelve Months Vapor Inhalation Study in Rats”

Sarhan

Jain

Mutwally

et al. 2019

Biology

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We investigated the early risk of developing cancer by inhalation of low doses (60 µL/day) of methyl tertiary butyl ether (MTBE) vapors using protein SDS-PAGE and LC-MS/MS analysis of rat sera. Furthermore, histological alterations were assessed in the trachea and lungs of 60 adult male Wistar rats. SDS-PAGE of blood sera showed three protein bands corresponding to 29, 28, and 21 kDa. Mass spectroscopy was used to identify these three bands. The upper and middle protein bands showed homology to carbonic anhydrase 2 (CA II), whereas the lower protein band showed homology with peroxiredoxin 2. We found that exposure to MTBE resulted in histopathological alterations in the trachea and the lungs. The histological anomalies of trachea and lung showed that the lumen of trachea, bronchi, and air alveoli packed with free and necrotic epithelial cells (epithelialization). The tracheal lamina propria of lung demonstrated aggregation of lymphoid cells, lymphoid hyperplasia, hemorrhage, adenomas, fibroid degeneration, steatosis, foam cells, severe inflammatory cells with monocytic infiltration, edema, hemorrhage. Occluded, congested, and hypertrophied lung arteries in addition, degenerated thyroid follicles, were observed. The hyaline cartilage displayed degeneration, deformation, and abnormal protrusion. In conclusion, our results suggest that inhalation of very low concentrations of the gasoline additive MTBE could induce an increase in protein levels and resulted in histopathological alterations of the trachea and the lungs.

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Association Between GSTP1 Ile105Val Genetic Polymorphism and Dependency to Heroin and Opium

Rezaei

Saadat

2018

Biochem Genet

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Relationship between glutathione S-transferase P1 (GSTP1, OMIM: 134660) variants and the risk of drug dependency is unknown. Chronic use of illegal drugs leads to oxidative stress, which can be alleviated by cellular detoxification mechanisms. There are several polymorphisms in the GSTP1, including Ile105Val (rs1695). This polymorphism leads to an Ile105Val amino acid change and may alter the GSTP1 enzyme activity. There is no study on the association between this polymorphism and risks of heroin (HD) or opium (OD) dependency. This paper consists of two case-control studies. The first study consisted of 442 HD subjects and 794 healthy controls. The second study consisted of 143 cases with OD and 565 healthy blood donors as controls. Genotyping were carried out using PCR based method. The Ile/Val (OR 0.84, 95% CI 0.65-1.07, P = 0.165) and Val/Val (OR 0.87, 95% CI 0.56-1.36, P = 0.879) genotypes did not show significant association with the risk of HD. Neither the Ile/Val (OR 0.72, 95% CI 0.49-1.06, P = 0.103) nor the Val/Val (OR 0.61, 95% CI 0.29-1.30, P = 0.209) was associated with the risk of OD. The GSTP1 Ile105Val polymorphism was not associated with the risk of dependency to opium and heroin.

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Study of liver function and expression of some detoxification genes in the male rats exposed to methyl-tertiary butyl ether

Cited by 6 publications

References 20 publications

Expression Levels of Some Detoxification Genes in Liver and Testis of Rats Exposed to a Single Dose of Methyl-Tertiary Butyl Ether

Expression Levels of Some Detoxification Genes in Liver and Testis of Rats Exposed to a Single Dose of Methyl-Tertiary Butyl Ether

Impact Effect of Methyl Tertiary-Butyl Ether “Twelve Months Vapor Inhalation Study in Rats”

Association Between GSTP1 Ile105Val Genetic Polymorphism and Dependency to Heroin and Opium

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