Study of Leaf Metabolome Modifications Induced by UV-C Radiations in Representative Vitis, Cissus and Cannabis Species by LC-MS Based Metabolomics and Antioxidant Assays

Abstract

UV-C radiation is known to induce metabolic modifications in plants, particularly to secondary metabolite biosynthesis. To assess these modifications from a global and untargeted perspective, the effects of the UV-C radiation of the leaves of three different model plant species, Cissus antarctica Vent. (Vitaceae), Vitis vinifera L. (Vitaceae) and Cannabis sativa L. (Cannabaceae), were evaluated by an LC-HRMS-based metabolomic approach. The approach enabled the detection of significant metabolite modifications in the three species studied. For all species, clear modifications of phenylpropanoid metabolism were detected that led to an increased level of stilbene derivatives. Interestingly, resveratrol and piceid levels were strongly induced by the UV-C treatment of C. antarctica leaves. In contrast, both flavonoids and stilbene polymers were upregulated in UV-C-treated Vitis leaves. In Cannabis, important changes in cinnamic acid amides and stilbene-related compounds were also detected. Overall, our results highlighted OPEN ACCESSMolecules 2014, 19 14005 phytoalexin induction upon UV-C radiation. To evaluate whether UV-C stress radiation could enhance the biosynthesis of bioactive compounds, the antioxidant activity of extracts from control and UV-C-treated leaves was measured. The results showed increased antioxidant activity in UV-C-treated V. vinifera extracts.

Multiomics in Grape Berry Skin Revealed Specific Induction of the Stilbene Synthetic Pathway by Ultraviolet-C Irradiation

“…On the other hand, metabolome analyses of various plants have also been reported (Saito and Matsuda, 2010). However, only a few studies reporting the grape metabolome detected more than 1,000 metabolite peaks Sternad Lemut et al, 2013;Marti et al, 2014). Other studies detected far fewer metabolite peaks and discussed only a small number of metabolites (Dai et al, 2013).…”

The Effect of Light Spectrum on the Morphology and Cannabinoid Content of Cannabis sativa L.

“…In contrast, none of the cannabinoids in fiber-type plants were affected by UV-B radiation. In a more recent study, hemp leaves were exposed to UV-C radiation and analyzed for changes in secondary metabolite biosynthesis [26]. While no remarkable change in the cannabinoid content was observed, significant increases in dehydrostilbenes and cinnamic acid amide derivatives were found.…”

Physicochemical Composition and Sensory Analysis of Whole Juice Extracted From Grapes Irradiated With Ultraviolet C

“…This technology can also stimulate the secondary metabolism of plants, favoring the production of defense chemicals without leaving residues that could be a risk to human health (MARTI et al, 2014;PINTO et al, 2016;50 SHEN et al, 2013).…”

“…In addition, there are only few studies on its use due the fact that it is a recent technology (MARTI et al, 2014;PINTO et al, 2016). Thus, the present study aimed to evaluate the postharvest effect of different doses of ultraviolet irradiation C (UV-C) on 'Trebbiano', 'Niagara Branca', 'Isabel' and 'Cabernet Sauvignon' grapes, as well as the effect of the storage period.…”