Study of internalization and viability of multimodal nanoparticles for labeling of human umbilical cord mesenchymal stem cells

Abstract

The stability of multimodal magnetic nanoparticles-Rhodamine B found in cultured Dulbecco's Modified Eagle's-Low Glucose medium and RPMI 1640 medium assured intracellular mesenchymal stem cells labeling. This cell labeling did not affect viability of labeled mesenchymal stem cells since they continued to proliferate for five days.

Umbilical cord mesenchymal stem cells labeled with multimodal iron oxide nanoparticles with fluorescent and magnetic properties: application for in vivo cell tracking

“…[16][17][18] Nanoparticles labeled with Rhodamine-B (Rh-B), a fluorescent dye that can be visualized by both MRI and fluorescent imaging, is an example of multimodal iron oxide nanoparticles (MION) with magnetic and fluorescent properties. 11,19,20 Multimodal imaging may be a powerful tool for in vivo studies of stem cell homing and injured tissue regeneration by fluorescence techniques. 11,19 This is because MIONs are not only nontoxic, but they also do not cause cell cycle arrest or changes in morphology or phenotype, and are visible by MRI and optical imaging without the requirement for invasive methods.…”

“…Accordingly, we suggest that MION-Rh dispersion has little influence on the stability of MION-RH in culture medium, where there is a slight imbalance of forces, such as attractive Van der Waals forces and repelling electrostatic and steric forces, which interact among MION-Rh, 38 ensuring the uptake efficiency of MION-Rh. 20 Thus, it seems likely that uptake of SPION depends on their size, and the aggregation resulting from the interacting forces between the SPION coating and the medium may determine the uptake efficiency. 39 Here we evaluated the colloidal stability of MION-Rh in both DMEM-LG and RPMI 1640 because these particles remain stable in both types of medium; however, DMEM-LG is the most often cited in the literature, so we chose to continue the experiments using DMEM-LG.…”

Methods of Granulocyte Isolation from Human Blood and Labeling with Multimodal Superparamagnetic Iron Oxide Nanoparticles

“…Regarding the stability of nanoparticles in the labeling process, previous studies by our group [90][91][92] evaluated SPION stability before labeling in different media and demonstrated that supplementation with 10% fetal bovine serum (FBS) contributed stabilized SPIONs in the culture medium during the labeling process [90,91,93]. This type of strategy was also reported in other studies [54,58,59,85,87], with FBS concentrations ranging from 5% to 20% during the labeling process of granulocytes or leucocytes with SPIONs.…”

Imaging modalities using magnetic nanoparticles – overview of the developments in recent years

“…They were able to trigger release of the drugs with radiofrequency heating, thereby combining hyperthermia with targeted chemotherapy [68] . Miyaki et al analyzed the suitability of multimodal magnetic nanoparticles labeled with rhodamine B for detection of cells in culture and demonstrated internalization of these nanoparticles as well as their stability and imaging properties [69] . Another recent publication deals with the attachment of DNA-binding fluorochromes to nanoparticles.…”

“…Jarrett et al [59] : Development of a PET/MRI probe using superparamagnetic iron oxide McCarthy et al [60] : Development of thrombosis-specific molecular imaging agents Skaat and Margel [61] : Selective marking of A β 40 fibrils using superparamagnetic iron oxide Purushotham and Ramanujan [62] : Synthesis of composite nanomaterials for hyperthermia and drug release Nowostawska et al [64] : Analysis of porhyrin magnetic nanoparticle composite Huang et al [65] : Characterization of anti-α -fetoprotein mediated Fe 3 O 4 Yiu et al [66] : Development of Fe 3 O 4 -PEI-RITC with MR-fluorescence imaging and transfections capabilities Ren et al [67] : Investigation of the efficiency of Fe 3 O 4 combined with chemotherapy and hyperthermia for overcoming multidrug resistance Xu et al [68] : Synthesis of few-layer, carbon-coated, iron magnetic nanoparticles for controlled drug release and hypothermia Miyaki et al [69] : Analysis of multimodal magnetic nanoparticles-rhodamine B Cho et al [70] : Development of nanoparticles that bind to DNA through fluorochome-mediated interactions MPI Weaver et al [98] : Exploration of the signal from magnetic nanoparticles Goodwill et al [77] : Introduction of narrowband-MPI Ferguson et al [78] : Presentation of mathematical modeling results that show the dependence of core design on physical properties Knopp et al [79] : Simulation study on different trajectories moving the field-free point through the field-of-view Knopp et al [80] : Calculation of the system function using a model of the signal chain Knopp et al [81] : Demonstration of how the quality of the least-squares solution can be improved Knopp et al [82] : Investigation of the spatial resolution of magnetic particle imaging Rahmer et al [83] : Presentation of a detailed analysis of a measured system function Finas et al [84] : Investigation of superparamagnetic iron oxide and magnetic particle imaging as sentinel lymph node biopsy tracer Goodwill et al [85] : " Derive " of 2-D x-space signal equation and 2-D image equation Reeves and Weaver [86] : Development and validation of a stochastic dynamical model of rotating Brownian nanoparticles from a Langevin equation approach Haegele et al [87] : Test of various commercially available catheters, guide wires, and a catheter experimentally coated with SPIONs regarding signal characteristics using magnetic particle spectroscopy Ferguson et al …”