Study of individual plant responsiveness in anther cultures of selected pepper (<i>Capsicum</i> spp.) genotypes

Niklas-Nowak, Aleksandra; Olszewska, Dorota; Kisiała, Anna; Nowaczyk, P.

doi:10.2478/v10245-012-0017-x

Cited by 15 publications

(13 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Anther culture is a widely used haploidization technique in pepper. Several studies have been carried out on the development of successful protocols related to pepper anther culture (Abak, 1983;Al Remi et al, 2014;Ata, 2011;Buyukalaca et al, 2004;Comlekcioglu et al, 2001;G emesn e Juh asz et al, 2001;George and Narayanaswamy, 1973;Harn et al, 1975;Niklas-Nowak et al, 2012;Novak, 1974;Olszewska et al, 2013;Saccardo and Devreux, 1974;Tas xkın et al, 2011;Wang et al, 1973). In these studies, the rate of spontaneous doubled haploidy was determined via ploidy analyses.…”

Section: Discussionmentioning

confidence: 99%

“…The success of these methods varies according to the species. Positive results in pepper have been achieved with anther culture (Al Remi et al, 2014;Buyukalaca et al, 2004;Comlekcioglu et al, 2001;Ercan et al, 2006;G emesn e Juh asz et al, 2001;Niklas-Nowak et al, 2012;Olszewska et al, 2013;€ Ozkum and Tıpırdamaz, 2002;Tas xkın et al, 2011). The advantage of this method is that there are thousands of microspores in each anther and numerous haploid plants can be obtained from a single anther.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Effect of Pepper Types on Obtaining Spontaneous Doubled Haploid Plants via Anther Culture

Keleş¹,

Pınar²,

Ata³

et al. 2015

horts

View full text Add to dashboard Cite

The most successful technique used to obtain haploid plant in pepper is anther culture. The chromosome content of haploid plants can be doubled spontaneously or using colchicine. In this study, we compared the rate of spontaneous doubled haploidy of different pepper types. Seven charleston, six bell, eight capia, and seven green pepper genotypes were used as plant material. Murashige and Skoog (MS) nutrient medium with 4 mg·L−1 naphthaleneacetic acid (NAA), 0.5 mg·L−1 6-benzylaminopurine (BAP), 0.25% activated charcoal, 30 g·L−1 sucrose, and 15 mg·L−1 silver nitrate (AgNO3) was used. Ploidy levels of plants obtained through anther culture were detected using both flow cytometry and simple sequence repeats (SSR) markers. The results showed that different spontaneous doubled haploidy rates were obtained from different pepper types. The highest rate was observed in bell pepper type with 53.4% (mean of six genotypes) of haploid plants undergoing spontaneous chromosome doubling. This was followed by charleston and capia types with 31.9% and 30.4% doubling, respectively. Green pepper type gave the lowest spontaneous doubled haploidy rate with 22.2% doubling. The results obtained from this study will be useful both for future work on haploidy in pepper and for breeding programs.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Effect of Pepper Types on Obtaining Spontaneous Doubled Haploid Plants via Anther Culture

Keleş¹,

Pınar²,

Ata³

et al. 2015

horts

View full text Add to dashboard Cite

show abstract

“…These factors include genotype, age of donor plant, pollen development stage, pretreatments made to buds or anthers, and composition of nutrient media. Successful outcomes were achieved with anther culture in pepper (Al Remi et al, 2014;Buyukalaca et al, 2004;Comlekcioglu et al, 2001;Ercan et al, 2006;G emesn e Juh asz et al, 2001;Keles x et al, 2015;Niklas-Novak et al, 2012;Olszewska et al, 2013;Tas xkin et al, 2011), eggplant (Bas xay andEllialtıo glu, 2013;Bas xay et al, 2011), and Brassica spp. (Achar, 2002;Arnison et al, 1990aArnison et al, , 1990bBiddington and Robinson, 1990;G orecka et al, 2007;Phippen and Ockendon, 1990;Roulund et al, 1990).…”

mentioning

confidence: 99%

First Report of Obtaining Haploid Plants Using Tissue Culture Techniques in Spinach

Keleş¹,

Özcan

Pınar

et al. 2016

horts

View full text Add to dashboard Cite

Monoic, dioic, and hermaphrodite flower types complicate spinach breeding and cultivar development. The availability of haploid plants will definitely accelerate spinach breeding; however, there are currently no reports in the literature about the use of tissue culture techniques to obtain spinach haploids. Therefore, in this study, pollination with irradiated pollen and anther culture methods were used to obtain haploid plants in spinach. For anther culture, three spinach varieties (Koto F1, Favorit F1, and Greenstar F1) and four different nutrient media were tested to obtain haploid embryos. Significant outcomes were not achieved from anther culture, and only two plants were obtained from all the experiments. Gynogenesis studies using irradiated pollen were performed with the same three spinach varieties and six gamma ray doses (100, 150, 200, 300, 400, and 500 Gy) from (cobalt60) Co60. Murashige and Skoog (MS) nutrient medium containing 1 mg·L−1 indoleacetic acid (IAA) was used for embryo germination. Current findings revealed that the varieties produced responded differently to the various doses of radiation. A total of 3414 embryos and 1710 plants were obtained from experiments carried out for 2 years. Considering the numbers of embryos and plants per 100 seeds, the Favorit F1 variety provided better results than the other two varieties. However, significantly different outcomes were not achieved with regard to irradiation doses. Embryos were observed at all doses tested. Flow cytometry analyses that were carried out on regenerated plants revealed whether the plants were diploid or doubled haploid, and molecular analyses revealed that diploids resulted from spontaneous chromosome doubling. The current findings offer significant results for spinach breeding using haploids.

show abstract

“…Unfortunately, none are applicable to a wide spectrum of genotypes. For example, Niklas-Nowak et al (2012) established different androgenic response rates even in individual plants belonging to the same genotype. Parra-Vega et al (2013b) obtained a higher percentage of embryos through the shed-microspore approach, compared to conventional anther culture.…”

Section: Introductionmentioning

confidence: 99%

“…In these early studies, the regeneration of plants achieved was of low efficiency, and research efforts in the next experiments aimed to identify the factors influencing induction of androgenesis. After many experiments, it was found that androgenic response depended on the following: growing conditions, age and genotype of the donor plant (Ercan et al, 2006;Niklas-Nowak et al, 2012;Grozeva et al, 2013;KolevaGudeva et al, 2013;Alremi et al, 2014), developmental stage of microspores in the anther (Nowaczyk and Kisiała, 2006;Parra-Vega et al, 2013a;Barroso et al, 2015), composition of the culture medium, concentration and combination of growth regulators, organic and inorganic additives (Büyükalaca et al, 2004;Zhao et al, 2010;Taşkin et al, 2011;Roshany et al, 2013;Olszewska et al, 2014), and pretreatment of flower buds and/or anthers (KolevaGudeva et al, 2007;Özkum and Tıpırdamaz, 2007;Irikova et al, 2011b;Nowaczyk et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Pepper (Capsicum annuum L.) anther culture: fundamentalresearch and practical applications

Irikova¹,

Kintzios²,

Grozeva³

et al. 2016

Turk J Biol

View full text Add to dashboard Cite

IntroductionIn general, haploid cells or individuals are those in which the original chromosome number has reduced by half. According to Seguí-Simarro (2010), androgenesis can be defined as a set of biological processes leading to an individual that genetically originated exclusively from a male nucleus. After spontaneous or induced genome doubling, double haploids (DHs) that are completely homozygous are obtained. The production of haploid plants can be induced by methods such as parthenogenesis, interspecific hybridization, pollination with irradiated pollen, hormonal and/or chemical treatment, and application of high-temperature stress (Forster et al., 2007;Murovec and Bohanec, 2013). In anther culture, haploids and DHs occur through direct microspore embryogenesis. This technique has no analogue for fast creation of pure homozygous lines in a single step. In pepper-plant breeding, for example, this method has helped in the development of important new varieties with improved adaptability, productivity, and disease and pest tolerance (Chunling and Baojun, 1995;Venczel and Mitykó, 1995;Arnedo Andrés et al., 2004;Nervo et al., 2007). DH lines are a suitable material for genetic and molecular studies, and this determines their high value in molecular breeding as well (Madhusudhana, 2015).Regarding the genus Capsicum, the first haploids were derived by in vitro anther culture of C. annuum and C.

show abstract

Study of individual plant responsiveness in anther cultures of selected pepper (Capsicum spp.) genotypes

Cited by 15 publications

References 18 publications

Effect of Pepper Types on Obtaining Spontaneous Doubled Haploid Plants via Anther Culture

Effect of Pepper Types on Obtaining Spontaneous Doubled Haploid Plants via Anther Culture

First Report of Obtaining Haploid Plants Using Tissue Culture Techniques in Spinach

Pepper (Capsicum annuum L.) anther culture: fundamentalresearch and practical applications

Contact Info

Product

Resources

About