Study ofYersinia pseudotuberculosisSurface Antigen Epitopes Using Monoclonal Antibodies

Immunochemical Nature of Receptors of Pseudotuberculosis Diagnostic Bacteriophage

“…Pretreatment with MAb 1-4 did not affect the effi ciency of phage adsorption to the cells. As has been previously shown [1], these mAb interact with non-identical epitopes located in different areas of O-side chains of Y. pseudotuberculosis LPS. Preliminary experiments showed that decreasing the temperature of cells growth on dense GM from 37°C to 10°C sharply reduced lytic capacity of pseudotuberculosis bacteriophage; this phenomenon was also observed for other S-forms of enterobacteria [11].…”

“…In the study of competition of the bacteriophage and antibodies for binding sites, we used cells grown at 37°C and characterized by low or no O-antigen production. Reducing the temperature of Y. pseudotuberculosis culturing to 27°C and lower increased expression of surface O-side chains [1]. This probably results in shielding of deep bacteriophage-binding sites and subsequent reduction of its lytic capacity.…”