Study of <i><scp>T</scp>oxocara</i> seroprevalence among patients with allergy and healthy individuals in <scp>B</scp>ulgaria

Kaneva, Eleonora; Rainova, Iskra; Harizanov, Rumen; Nikolov, Georgi; Kaftandjiev, Iskren; Mineva, Iva

doi:10.1111/pim.12217

Cited by 7 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Toxocariasis is a parasitic zoonotic disease with various clinical manifestations, protracted course, and common recurrences. In Bulgaria the exact incidence of human toxocariasis still not established, but significant information has been gathered over the last 10 years [24].…”

Section: Discussionmentioning

confidence: 99%

Human Cystic echinococcosis, Trichinellosis and Toxocariasis in Bulgaria: an update of data for 2015-2017

Harizanov¹,

Rainova²,

Kaftandjiev³

2019

Int Med

View full text Add to dashboard Cite

Background: The purpose of our study is to perform a retrospective analysis of the prevalence of some zoonotic helminth infections (trichinellosis, cystic echinococcosis, and toxocariasis) for the period 2015-2017, given their important medical and social significance for the public health system in Bulgaria and other endemic countries.Methods: We used data from the National Centre of Infectious and Parasitic Diseases and Regional Health Inspectorates for all registered cases of cystic echinococcosis and trichinellosis during the study period and data from the Department of Parasitology and Tropical Medicine on all cases seropositive for toxocariasis.Results: For the study period in the country were reported 800 confirmed cases of hydatid disease of which 715 (89.4%) primary and 85 (10.6%) cases of relapse. The group of children and adolescents accounted for 21% of the total number of infected. Ten trichinellosis outbreaks were also recorded, involving the population of 16 settlements across 10 of the 28 districts of the country. Contaminated with Trichinella larvae meat was consumed by 293 persons of whom laboratory confirmed infection was found in 113 individuals (38.6%). For the period, 408 persons with clinical symptoms suspicious for toxocariasis were tested and 78 (19.1%) of them were positive for the presence of specific anti-Toxocara antibodies.Conclusions: Parasitic zoonotic diseases have endemic spread in many countries around the world and pose a problem for public health. We present data on three helminth diseases, two of which (cystic echinococcosis and trichinellosis) are still widespread in Bulgaria and are of great medical and social importance.

show abstract

Section: Discussionmentioning

confidence: 99%

Human Cystic echinococcosis, Trichinellosis and Toxocariasis in Bulgaria: an update of data for 2015-2017

Harizanov¹,

Rainova²,

Kaftandjiev³

2019

Int Med

View full text Add to dashboard Cite

show abstract

“…Data published on toxocariasis show a widespread prevalence of this helminthosis, which is still a neglected and poorly evaluated problem (22). Our studies among healthy individuals in Bulgaria show 8.0% prevalence of Toxocariasis found in ELISA, and and 4.0% in WB (23). Studies on the prevalence of T. canis in animals in our country show that this is one of the most common infestations in dogs (12%) (24), especially in small puppies.…”

Section: Discussionmentioning

confidence: 61%

Application of PCR Method for Detection and Species Identification of Toxocara Spp.

Kaneva

Tsvetkova

Raynova

2020

Probl Infect Parasit Dis

View full text Add to dashboard Cite

The ascaridoid nematodes of dogs and cats T. canis and T. cati are with widespread distribu-tion and causative agents of a disease in human and animals named Toxocariasis. Human dis-ease has several clinical forms with different clinical manifestation such as visceral, ocular, neurotoxocariasis and covert toxocariasis. The morphological methods used to differentiate the two species, especially to identify eggs or larvae, can lead to inaccurate diagnosis. This requires the use of more reliable methods, such as PCR, for identification of Toxocara species. The aim of our research is to develop in our conditions a PCR method for species identification of Toxocara and to determine its applicability on different stages of parasites. The method used by Khademvatan et al. (2013), we performed with some modifications in different forms of Toxocara - eggs, larvae and adult parasites. We used species-specific oligonucleotide primers from the ITS2 gene sequence of the ribosomal DNA - Tcan1/NC2 for T. canis and Tcat1/NC2 for T. cati. The presence of a band with a size of 380 bp, specific for T. canis, was found for all stages of the studied parasite. The described method will allow species differentiation of Toxocariasis causative agents and improve the diagnosis of the disease, as well as determine the actual spread and reservoirs of these parasites.

show abstract

“…In a previous study of 50 clinically healthy subjects (32 men and 18 women), we found 6% (n = 3) seroprevalence for toxocariasis in ELISA and 4% (n = 2) in WB [31]. Comparison among the group of the patients with inflammatory heart disease and the group of clinically healthy blood donors showed relatively higher prevalence of specific anti-Toxocara antibodies in the first group (7.3% versus 6% in ELISA and 7.3% vs. 4% in WB), but the positive serology is not sufficient to provide a definitive diagnosis and the statistical comparison showed no significant difference (2X2 contingency table, Fisher's exact test, p=0.654).…”

Section: Discussionmentioning

confidence: 68%

Determination of Specific Anti - Toxocara Antibodies in Patients with Cardiac Disease

Kaneva

Harizanov

Krumova

et al. 2021

ABCMed

View full text Add to dashboard Cite

Aim of this study is to find the seroprevalence of specific anti - Toxocara IgG antibodies among patients with inflammatory heart disease and to evaluate the significance of this parasite as a possible etiological agent of such pathology. We performed a serological study of 41 patients with heart disease (myocarditis, pericarditis and endocarditis) for presence of specific anti-Toxocara IgG antibodies. We used ELISA for primary screening, and Western blot as a confirmatory method. Presence of specific anti-Toxocara IgG antibodies in ELISA was detected in three (7.3%) of the serum samples, and another two (4.9%) were with borderline values. These serum samples were further examined in Western blot and three of them (7.3%) displayed disease-specific bands. We do not in any way claim that in these cases Toxocara infection is the cause of inflammatory heart disease, but the data from the study shows that such a link is possible.

show abstract

Study of Toxocara seroprevalence among patients with allergy and healthy individuals in Bulgaria

Cited by 7 publications

References 21 publications

Human Cystic echinococcosis, Trichinellosis and Toxocariasis in Bulgaria: an update of data for 2015-2017

Human Cystic echinococcosis, Trichinellosis and Toxocariasis in Bulgaria: an update of data for 2015-2017

Application of PCR Method for Detection and Species Identification of Toxocara Spp.

Determination of Specific Anti - Toxocara Antibodies in Patients with Cardiac Disease

Contact Info

Product

Resources

About