Study of honeybee caste differentiation by glucose level differences during development

Ishay, Jacob S.; Fischl, J.; Alpern, G.

doi:10.1007/bf02283903

Cited by 6 publications

(2 citation statements)

References 12 publications

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“…In support of this, important carbohydrate metabolism and energy production metabolic proteins like aldehyde dehydrogenase, transketolase, ATP synthase ßsubunit, phosphoglycerate mutase and enolase showed considerable age-linked increase in queen destined larvae. Together with functional enrichment and biological network analysis, our data suggests that carbohydrate metabolism and energy production proteins play special and central roles in caste differentiation and determination [23], [25], [26]. Moreover, especially for the queen larvae, phosphoglycerate kinase and aldehyde dehydrogenase became apparent only at 120 hours and enolase, phosphoglycerate mutase and transketolase were present at both developmental stages.…”

Section: Discussionmentioning

confidence: 81%

Differential Protein Expression in Honeybee (Apis mellifera L.) Larvae: Underlying Caste Differentiation

Rundassa

et al. 2010

PLoS ONE

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Honeybee (Apis mellifera) exhibits divisions in both morphology and reproduction. The queen is larger in size and fully developed sexually, while the worker bees are smaller in size and nearly infertile. To better understand the specific time and underlying molecular mechanisms of caste differentiation, the proteomic profiles of larvae intended to grow into queen and worker castes were compared at 72 and 120 hours using two dimensional electrophoresis (2-DE), network, enrichment and quantitative PCR analysis. There were significant differences in protein expression between the two larvae castes at 72 and 120 hours, suggesting the queen and the worker larvae have already decided their fate before 72 hours. Specifically, at 72 hours, queen intended larvae over-expressed transketolase, aldehyde reductase, and enolase proteins which are involved in carbohydrate metabolism and energy production, imaginal disc growth factor 4 which is a developmental related protein, long-chain-fatty-acid CoA ligase and proteasome subunit alpha type 5 which metabolize fatty and amino acids, while worker intended larvae over-expressed ATP synthase beta subunit, aldehyde dehydrogenase, thioredoxin peroxidase 1 and peroxiredoxin 2540, lethal (2) 37 and 14-3-3 protein epsilon, fatty acid binding protein, and translational controlled tumor protein. This differential protein expression between the two caste intended larvae was more pronounced at 120 hours, with particular significant differences in proteins associated with carbohydrate metabolism and energy production. Functional enrichment analysis suggests that carbohydrate metabolism and energy production and anti-oxidation proteins play major roles in the formation of caste divergence. The constructed network and validated gene expression identified target proteins for further functional study. This new finding is in contrast to the existing notion that 72 hour old larvae has bipotential and can develop into either queen or worker based on epigenetics and can help us to gain new insight into the time of departure as well as caste trajectory influencing elements at the molecular level.

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Section: Discussionmentioning

confidence: 81%

Differential Protein Expression in Honeybee (Apis mellifera L.) Larvae: Underlying Caste Differentiation

Rundassa

et al. 2010

PLoS ONE

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“…The only consistent finding is that WJ and RJ differ in sugar concentration, which may trigger different developmental programs. RJ usually has 12% sugar (mostly glucose and fructose), whereas WJ contains 4% (Corona et al, 1999;Ishay et al, 1976). If both queen larvae and worker larvae are present in a colony, the same nurse bee can feed a queen larva, and then immediately begin feeding a worker larva.…”

Section: Nurse Feeding Programmentioning

confidence: 97%

Physiological and Molecular Mechanisms of Nutrition in Honey Bees

Wang

Li-Byarlay

2015

Advances in Insect Physiology

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Étude comparative au moyen d'or radio-actif de l'alimentation des larves d'ouvrières et des larves de reine chez la FourmiPlagiolepis pygmaea Latr.

Bonavita-Cougourdan

Passera

1978

Ins. Soc

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Study of honeybee caste differentiation by glucose level differences during development

Cited by 6 publications

References 12 publications

Differential Protein Expression in Honeybee (Apis mellifera L.) Larvae: Underlying Caste Differentiation

Differential Protein Expression in Honeybee (Apis mellifera L.) Larvae: Underlying Caste Differentiation

Physiological and Molecular Mechanisms of Nutrition in Honey Bees

Étude comparative au moyen d'or radio-actif de l'alimentation des larves d'ouvrières et des larves de reine chez la FourmiPlagiolepis pygmaea Latr.

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