Study of H/D-exchange reaction kinetics of polypeptides

Hydrogen/deuterium exchange mass spectrometry in the world of small molecules

“…Very early on, the quadrupole RF‐only collision cell of the TQ was used to produce ion/molecule reactions with low‐kinetic‐energy selected ions from the first quadrupole (White, 1987). The RF‐only multipole collision cell of triple quadrupoles (or Qq/TOF with segmented quadrupole; Chudinov et al, 2010) is particularly used for reactive collisions such as HDXs. This approach was soon applied to HDX processes of the deprotonated o , m , and p dichlorobenzene isomers (noted as [M i −H] − ) with i = o , m and p ) with the D2O and ND3 reagents yielding up to three HDXs which were investigated according to the considered isomers.…”

“…The results are in agreement with the FT/ICR results, again suggesting the occurrence of a relay mechanism for the HDXs. Using a modified segmented quadrupole with ND3 as reagent diluted in N 2 , the length of reaction increased from 30 ms to 1 s. Based on statistical methods, the HDX rate constants of different mobile protons of protonated Leu‐enkephalin, gramicidin S, and apamin were distinguished (Chudinov et al, 2010). Protonated A 2 RA 2 , Ac‐A 2 RA 2 , A 2 RA 2 ‐OMe were submitted to HDX with D 2 O.…”

Mass Spectrometry in Russia

“…260 It was based on the monitoring of mass spectra of biomolecules during exchange reactions and estimating the exchange probabilities for each H-atom at a fixed time. Another team from the same Institute has studied the H/D-exchange reaction kinetics in polypeptide molecules by using D 2 O or ND 3 and a highresolution orthogonal time-of-flight mass spectrometer 261 or the segmented RF-quadrupole as an ion-molecule reactor. 262 A principal possibility to distinguish different mobile H-atoms in peptides using a statistical approach and reaction rates has been shown.…”