Study of Growth Parameters on Apricot Shoot-Tip Grafting in Vitro (Stg)

Deogratias, Jean-Marc; Castellani, Valérie; Dosba, F.; Juárez, José; Arregui, J. M.; Ortega, Cristian Valarezo; Ortega, Victoria; Llácer, G.; Navarro, Luís

doi:10.17660/actahortic.1991.293.43

Cited by 6 publications

(6 citation statements)

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“…Moreover, In vitro culture derived shoot-tips had better response compared to in vivo derived counterparts. These findings are consistent with the results in apple (Deogratias et al, 1991) peach, plum and apricot (Edriss et al,1984 andEstrada-Luna et al,2002).…”

Section: Micrograftingsupporting

confidence: 93%

EVALUATION OF GROWTH PARAMETERS OF BOTH DOG RIDGE, SALT CREEK ROOTSTOCKS AND SHOOT TIP MICRO-GRAFTING OF SUPEROIR AND THOMPSON SEEDLESS CULTIVARS Through in vitro CULTURE

El-Hammady¹,

El-Morshedy²,

Awad³

2012

Journal of Plant Production

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This investigation was carried out to establish a suitable growth media for two grape rootstocks namely, Salt Creek and Dog Rige and to evaluate micrografting of Thompson seedless and superior cultivars on these stocks through in vitro culture. Plant height, leaves and shoot number increased with increasing the concentrations of 2ip from 3 to 7M. Different concentrations of BAP and GA3 showed that rootstock responded higher height than concentration of BAP only, except 4 M BAP associated with Dogridge which had significant effect than all combinations. On the other hand, Salt Creek grown in media supplemented with (0.5 M IBA) had the lowest root length.Micrografting of Thompson seedless and Superior cultivars were evaluated using two methods of micro-grafting (shoot-tip and cleft) under in vitro conditions on the studied rootstocks. Shoot-tips had higher successful (take) of graft union compared to cleft method which failed to introduce any successful plantlet. Micrografting derived from shoot-tip explants of the two grapevine cultivars showed satisfactory growth characteristics (plantlet height, leaf number and shoot number). Whereas, significant difference between the cultivars and the rootstocks regarding successful graft union and subsequent growth related traits were obtained. Salt Creek rootstock gave the higher rate of success of two scions, respectively if compared to Dog Ridge for both scions. Moreover, shoot length of Thompson seedless scion on Salt Creek rootstock was higher than Thompson seedless scion on Dog Ridge rootstock. In addition, superior cultivar on both of Salt Creek and Dog Ridge showed the same trend. In conclusion, micro-grafting proved to be an alternative suitable propagation method leading to vigorous growth potential of grafted cultivars.

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Section: Micrograftingsupporting

confidence: 93%

EVALUATION OF GROWTH PARAMETERS OF BOTH DOG RIDGE, SALT CREEK ROOTSTOCKS AND SHOOT TIP MICRO-GRAFTING OF SUPEROIR AND THOMPSON SEEDLESS CULTIVARS Through in vitro CULTURE

El-Hammady¹,

El-Morshedy²,

Awad³

2012

Journal of Plant Production

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“…The origins and type of scion material are some of the determinants in successful micrografting [34,[52][53][54]. The scions used in micrografting, usually shoots or shoot tips, can be obtained from in vitro or ex vitro grown plants.…”

Section: Preparation Of Scionsmentioning

confidence: 99%

“…The scions used in micrografting, usually shoots or shoot tips, can be obtained from in vitro or ex vitro grown plants. Scion material has traditionally been sourced from in vitro plants, having the advantage of being free from fungal and bacterial contaminations, the desired size, and being available year-around [35,53,55]. The use of ex vitro material, however, may introduce a seasonality component to the procedure, as the excised plant material may remain in a dormant stage [53].…”

Section: Preparation Of Scionsmentioning

confidence: 99%

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

et al. 2022

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In vitro micrografting is an important technique supporting the micropropagation of a range of plant species, particularly woody plant species. Over the past several decades, in vitro micrografting has become a strategy to facilitate shoot recovery and acclimatization of in vitro-grown horticultural species. This review focuses on studies on horticultural crops over the past two decades that cover the establishment of in vitro micrografting, discusses factors affecting the success of in vitro micrografting, and provides commentary on the contribution of micrografting applications to the field of micropropagation. Considering the important roles of micrografting in the restoration of vigor and rooting competence, in promotion of shoot recovery following somatic embryogenesis and organogenesis, and in facilitation of shoot regrowth after cryopreservation, the potential use of this technique in facilitation of genetic engineering and safe conservation of horticultural species are specially highlighted.

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“…A mechanically strong graft union was formed during the course of a three week subculture of micrografts in a liquid medium without the addition of growth regulators. Deogratias et al [15] obtained best results in micrografting of apricot when cultured in medium comprising MS salts with 3 per cent sucrose.…”

Section: Media Usedmentioning

confidence: 99%

Micrografting of Fruit Crops-A Review

MIS¹

2015

Horticulture

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Micrografting was developed in 1980s and consists of the placement in aseptic conditions of a maintained scion onto an in vitro grown rootstock. The results of in vitro micrografting and the plant material derived from it can be further multiplied in tissue culture conditions or acclimatized to outdoor conditions. Micrografting is a technique that potentially can combine the advantages of rapid in vitro multiplication with increased productivity that results from grafting, superior rootstock and scion combinations. Among various methods of micrografting, slit or wedge grafting has been found most suitable in case of fruit crops. In vitro shoot tips are better as compared to in vivo shoot tips for carrying out micrografting resulting in higher graft success, less contamination, less vitrification, lower shoot tip necrosis and good vigour of micrografts. Media used for growing micrografted plantlets also affects grafting success, vitrification, contamination and vigour. Use of appropriate medium depends on genotype and growing conditions. Modification of basal medium formulation has been an effective means for achieving good graft success. The sucrose concentration of the nutrient medium of grafted plants has been found to play a significant role. Besides these, scion length and rootstock development stage also influences graft success, vitrification, shoot tip necrosis, vigour and contamination. The use of pre-treated shoot apices not only markedly increases the grafting success but also overcome problems encountered during the handling of in vitro micrografting that results in browning and drying of apices.

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Study of Growth Parameters on Apricot Shoot-Tip Grafting in Vitro (Stg)

Cited by 6 publications

References 0 publications

EVALUATION OF GROWTH PARAMETERS OF BOTH DOG RIDGE, SALT CREEK ROOTSTOCKS AND SHOOT TIP MICRO-GRAFTING OF SUPEROIR AND THOMPSON SEEDLESS CULTIVARS Through in vitro CULTURE

EVALUATION OF GROWTH PARAMETERS OF BOTH DOG RIDGE, SALT CREEK ROOTSTOCKS AND SHOOT TIP MICRO-GRAFTING OF SUPEROIR AND THOMPSON SEEDLESS CULTIVARS Through in vitro CULTURE

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

Micrografting of Fruit Crops-A Review

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