Study of Green Nanoparticles and Biocomplexes Based on Exopolysaccharide by Modern Fourier Transform Spectroscopy

Abstract

The intention of this chapter is to contribute in clarification of nanoparticle synthesis and biocomplexes based on exopolysaccharide, green synthetic method development, their physico-chemical characterization by modern spectroscopy, as well as testing of their antimicrobial activity. Silver nanoparticles of polysaccharide type have scientific interest, but practical importance too, because of their application in pharmaceutical and cosmetic product development due to proven antimicrobial and antioxidant activities. On the other hand, the biocomplexes based on exopolysaccharides are important in treatment of biometal deficiency in human and veterinary medicine, as well as in metal ion transporting in organism. Despite a number of studies of this kind of complexes, the investigations of effect of their structure to pharmaco-biological activity are still interesting. It is important that question of interaction between reducing and stabilizing agents with metal ions is still opened. In this respect, the presented chapter offers further progress in the examination of silver nanoparticles and cobalt biocomplex synthesis with dextran oligosaccharides and its derivatives (such as dextran sulfate and carboxymethyl dextran). The complex structure, spectroscopic characterization, and the spectra-structure correlation have been analyzed by different Fourier transform infrared (FTIR) spectroscopic techniques combined with energy-dispersive X-ray (EDX), X-ray diffraction (XRD), scanning electron microscopy (SEM), and surface plasmon resonance UV-Vis methods.

Engineering of inhalable nano-in-microparticles for co-delivery of small molecules and miRNAs

“…The bands at around 804 cm −1 obtained from the asymmetric S-O-S vibration and the asymmetric and symmetric SOO − stretching vibrations at 1230 cm −1 and 987 cm −1 confirmed the presence of the sulfate group in the DS spectrum [29]. The tPENs' spectra analysis revealed peaks at 1117 cm −1 and 1147 cm −1 , which were associated with P=O in TPP [22] and the production of sulfones [30], respectively. Additionally, tPENs displayed sharp bands with high intensities at 924 cm −1 , 1014 cm −1 (sulfo-group), 1410 cm −1 (symmetric stretching of COO −), 1570 cm −1 (Amide II band), 1637 cm −1 (C=O (amide I band)), 3286 cm −1 and 3178 cm −1 (O-H (H-bonded)), and 3421 cm −1 (primary amines).…”

Nanoparticle-Based Rifampicin Delivery System Development

“…An FT-IR spectrum of PEI exhibited the characteristic absorption peaks of amine groups (3363 cm −1 and 1647 cm −1 ), C–N stretching (1113 cm −1 ), C–H deformation (1471 cm −1 ), N–H deformation (1608 cm −1 ), and the stretching vibration of C-H bonds of the alkyl chain (2954 and 2843 cm −1 ) [ 26 ]. The spectra analysis of the APENs and WPENs showed the peaks of sulphones (1147 cm −1 ), the sulfo-group (924 cm −1 , 1014 cm −1 and 1043 cm −1 ) and weak N–H stretching vibrations (648 cm −1 ) [ 35 ]. High intensity of a few corresponding sharp bands, including 1570 cm −1 (N–H bending of amine) and 1410 cm −1 (symmetric CH 3 bending), was also observed, which can be attributed to polyplex formation via great electrostatic interaction.…”

Elaboration of Nanostructured Levan-Based Colloid System as a Biological Alternative with Antimicrobial Activity for Applications in the Management of Pathogenic Microorganisms

“…The broad asymmetric νC-H stretching peaks at 2935 cm −1 and symmetric peaks at 2885 cm −1 in S. salivarius K12 and L. mesenteroides DSM 20343 correspond to the methylene group CH 2 [47,48]. A broad νC-H stretching absorption band with an asymmetric peak at 2927.94 cm −1 was distinguished in the DEX spectrum [49,50]. The stretching vibration of the existence of νC-H and the broad band at 1647 cm −1 in all EPSs analyzed specify residual water presence [48,51].…”

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