Study of gene expression of Cytokine Genes (TLR-4, NOD-2) in patients with Otitis Media in Al-Najaf Governorate, Iraq

Abstract

The study aimed to evaluate the gene expression of genes (TLR-4,NOD-2) in patients of Otitis media and healthy persons. This finding included 50 samples that collected from healthy subject and 100 samples from a patients suffering from otitis media who attended Al-Sadr Medical City (ENT Department) in Al-Najaf Governorate during the period from February 2022 to June 2022. The samples had an average age ranging from 5 to 70 years. The gene expression of these genes among those suffering from Otitis media and healthy individuals have been investigated in this case-control research. Using a PCR technology. polymerase chain reactions were carried out to amplify each sample for the patient and control groups. The results of the molecular study (gene expression) showed a high significant increase in the level of gene expression in patients for the two genes NOD-2, TLR-4 genes (14.78 ± 2.369, 16.42 ± 3.158), respectively, with a significant difference at P≤0.05. TLR-4, NOD-2 as used as a molecular diagnosis Otitis Media patients.