Study of fish products in Metro Vancouver using DNA barcoding methods reveals fraudulent labeling

Hu, Yaxi; Huang, Shr Yun; Hanner, Robert; Levin, Julia; Lu, Xiaonan

doi:10.1016/j.foodcont.2018.06.023

Cited by 79 publications

(62 citation statements)

References 26 publications

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“…For these reasons, classical DNA barcoding methodologies as the one used in the present study are still of high preference by the researchers (e.g. Hu, Huang, Hanner, Levin, & Lu, ).…”

Section: Discussionmentioning

confidence: 99%

Using genetic methods for analysis of fish meals and feeds employed in Greek mariculture

Vlachavas¹,

Kokokiris

Zampeta³

et al. 2018

Aquac Res

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Large quantities of high protein fish meals are needed to sustain cultured species and thus the impact to marine ecosystem has been highly discussed. The aim of this study was to apply a PCR-cloning methodology for a robust insight into the composition of commercial fish meals and feeds for farmed species of the Greek mariculture, assessing the risk posed by aquaculture to marine ecosystems but also the risk posed by commercial fish feeds to the increase in trophic level of species farmed in Greece. 89% of the sequences were identified to species level and only 11% to genus/family level. Overall, a total of 49 taxa were identified (44 fish species/taxon, five non-fish species/taxon). Even though small pelagic fish like Engraulis sp. were the main portion, a wide range of species constituted the fish meals and feeds. Plant and animal species were also detected as an alternative protein source. Feed products employed in Greek mariculture still contain large portions of fish meals which increase the mean trophic level of farmed species causing a farming up trend. The results emphasize that such molecular methodologies are needed to certify aquafeeds allowing fish feed producers to demonstrate their commitment to sustainable aquaculture. K E Y W O R D S16s rRNA gene, fish feed, fish meal, PCR-cloning

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“…For these reasons, classical DNA barcoding methodologies as the one used in the present study are still of high preference by the researchers (e.g. Hu, Huang, Hanner, Levin, & Lu, ).…”

Section: Discussionmentioning

confidence: 99%

Using genetic methods for analysis of fish meals and feeds employed in Greek mariculture

Vlachavas¹,

Kokokiris

Zampeta³

et al. 2018

Aquac Res

View full text Add to dashboard Cite

show abstract

“…This is because the source (e.g., aquaculture, wild, fresh water, etc.) and identification of fish and seafood products (species, populations-linked to geographic origin) are highly complex [23][24][25][26]. In both fish and seafood products, the wide biological diversity, the removal of external features during processing steps, as well as the close phylogenetically relationships among them render the morphological identification almost impossible [27,28].…”

Section: Introductionmentioning

confidence: 99%

How Fishy Is Your Fish? Authentication, Provenance and Traceability in Fish and Seafood by Means of Vibrational Spectroscopy

Power

Cozzolino

2020

Applied Sciences

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Food authenticity, traceability and provenance are emerging issues of major concern for consumers, industries and regulatory bodies worldwide. In addition, both food safety and security are an intrinsic component of food quality where the above issues are key in modern traceability and management systems. It has been reported that substitution of a high-quality species by less expensive ones might be a frequent practice in seafood products such as fish and shellfish. In this type of products, the source (e.g., origin) and identification of the species are complex. Although different countries have implemented strict regulations and labelling protocols, these issues still are of concern. This article briefly reviews some of the most recent applications of vibrational spectroscopy (near and mid infrared, Raman) combined with chemometrics to target some of these issues in the seafood and fish industries.

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“…The adulterated species for S. salar were further expanded to O. keta and O. masou in a recent investigation in Madrid restaurants, 8 and to O. nerka in another survey conducted in Canada. 9 While in the USA, S. salar was found to be sold as Oncorhynchus species, and species substitution among various Oncorhynchus species was also revealed. 10 So far, few investigations have been made in China, the world's largest fish consumer, where the demand for salmonids has been growing over the last few years.…”

Section: Introductionmentioning

confidence: 99%

Rainbow trout (Oncorhynchus mykiss) identification in processed fish products using loop‐mediated isothermal amplification and polymerase chain reaction assays

Xiong

Huang

et al. 2020

J Sci Food Agric

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BACKGROUND Financial loss and health risk caused by the substitution of rainbow trout for other salmonid species have become a common issue around the world. The situation could be further exacerbated in China by the ‘abused’ common name of San Wen Yu (the corresponding Chinese ideogram 三文鱼) for salmonids, considering the absence of a standardized naming system for seafood species. To prevent such episodes, the present study aimed to develop novel loop‐mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assays targeting the mitochondrial cytochrome b gene for rapid identification of rainbow trout in processed fish products. RESULTS Rainbow trout‐specific primers (LAMP and PCR) were designed, and the specificity against 23 different fish species was confirmed. The minimum amount of detectable DNA for LAMP assay reached 500 pg, up to 10‐fold less than for PCR assay. In addition to agarose gel electrophoresis, naked‐eye inspection of the LAMP‐positive samples using SYBR Green I under daylight or ultraviolet light was also validated. Finally, commercial San Wen Yu products made from rainbow trout could be accurately identified using the newly developed LAMP and PCR assays, further cross‐confirmed by mini DNA barcoding and neighbor‐joining dendrograms. CONCLUSIONS The LAMP and PCR assays established in the study allow a fast and accurate identification of rainbow trout in processed fish products. © 2020 Society of Chemical Industry

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Study of fish products in Metro Vancouver using DNA barcoding methods reveals fraudulent labeling

Cited by 79 publications

References 26 publications

Using genetic methods for analysis of fish meals and feeds employed in Greek mariculture

Using genetic methods for analysis of fish meals and feeds employed in Greek mariculture

How Fishy Is Your Fish? Authentication, Provenance and Traceability in Fish and Seafood by Means of Vibrational Spectroscopy

Rainbow trout (Oncorhynchus mykiss) identification in processed fish products using loop‐mediated isothermal amplification and polymerase chain reaction assays

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