Study of fibrinogen adsorption on hydroxyapatite and TiO2 surfaces by electrochemical piezoelectric quartz crystal impedance and FTIR–ATR spectroscopy

Qin, Yang; Zhang, Youyu; Liu, Meiling; Ye, Min; Zhang, Yuqin; Yao, Shouzhuo

doi:10.1016/j.aca.2007.06.025

Cited by 35 publications

(21 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The prevalence of one or the other depends on the protein structure and the physico‐chemical features of the inorganic surface. For apatites is generally accepted that the main interactions are hydrophobic and electrostatic 2, 38. However, OHAp present a hydrophilic character (θ < 60)13 and in hydrophilic surfaces, repulsive solvation forces arising from strongly bound water occurs and they should avoid protein binding 39, 40.…”

Section: Discussionmentioning

confidence: 99%

Influence of surface features of hydroxyapatite on the adsorption of proteins relevant to bone regeneration

Moraleda¹,

Román²,

Rodríguez‐Lorenzo³

2013

J Biomedical Materials Res

View full text Add to dashboard Cite

Protein–surface interaction may determine the success or failure of an implanted device. Not much attention have been paid to the specific surface parametes of hydroxyapatite (OHAp) that modulates and determines the formation and potential activity of the layer of proteins that is first formed when the material get in contact with the host tissue. the influence of specific surface area (SSA), crystallite size (CS) and particle size (PS) of OHAp on the adsorption of proteins relevant for bone regeneration is evaluated in this article. OHAp have been prepared by a wet chemical reaction of Ca(OH)2 with H3PO4. One set of reactions included poly acrylic acid in the reactant solution to modify the properties of the powder. Fibrinogen (Fg) Fraction I, type I: from Human plasma, (67% Protein), and Fibronectin (Fn) from Human plasma were selected to perform the adsorption experiments. The analysis of protein adsorption was carried out by UV/Vis spectrometry. A lower SSA and a different aspect ratio are obtained when the acrylic acid is included in the reaction badge. The deconvolution of the amide I band on the Raman spectra of free and adsorbed proteins reveals that the interaction apatite–protein happens through the carboxylate groups of the proteins. The combined analysis of CS, SSA and PS should be considered on the design of OHAp materials intended to interact with proteins. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

show abstract

Section: Discussionmentioning

confidence: 99%

Influence of surface features of hydroxyapatite on the adsorption of proteins relevant to bone regeneration

Moraleda¹,

Román²,

Rodríguez‐Lorenzo³

2013

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…Short peptide sequences interact with TiO 2 surface via electrostatic interaction also [57][58][59]. Yang et al [61] and Chen et al [62] stated that electrostatic interaction may be the main interaction between fibrinogen and TiO 2 surfaces. Our results also indicate that electrostatic interaction and VDW interaction (including HB interaction) may be the main interactions between RGD and hydrophilic TiO 2 surfaces.…”

Section: Adsorption Mechanism Of Rgdmentioning

confidence: 99%

“…Adsorption of protein on various materials has been widely studied and it has been found that factors such as electrostatic interaction, hydrophobic interaction, hydrogen bonding, VDW interaction and specific chemical interactions between protein and the adsorbent play important roles [56][57][58][59][60][61][62][63][64][65][66][67][68][69][70][71]. Short peptide sequences interact with TiO 2 surface via electrostatic interaction also [57][58][59].…”

Section: Adsorption Mechanism Of Rgdmentioning

confidence: 99%

Adsorption of tripeptide RGD on rutile TiO2 nanotopography surface in aqueous solution

et al. 2010

View full text Add to dashboard Cite

“…5A), it was evident that HUVEC could sprout from microbeads into the surrounding matrix over a week in culture, and nascent vessel networks were observed. Culture of microbeads within bulk fibrin hydrogels is used to recreate a 3D matrix to mimic the in vivo tissue environment, and similar systems have been employed to study angiogenesis and vasculogenesis [39, 52, 54]. When microbeads were cultured as discrete units without being embedded in a surrounding hydrogel (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…It does not permit cell attachment, and therefore is often used in combination with other materials that facilitate cell adhesion and proliferation [36–38]. Hydroxyapatite (HA) is a main component of mineralized biological tissues that has been shown to adsorb and retain proteins [39–41], and may also promote vasculogenesis [42–44]. Fibrinogen (FGN) is a circulating precursor of the blood clotting protein fibrin, which is known to bind growth factors and proteins through its heparin-binding domain [45].…”

Section: Introductionmentioning

confidence: 99%

Distributed vasculogenesis from modular agarose-hydroxyapatite-fibrinogen microbeads

et al. 2017

View full text Add to dashboard Cite

Critical limb ischemia impairs circulation to the extremities, causing pain, disrupted wound healing, and potential tissue necrosis. Therapeutic angiogenesis seeks to repair the damaged microvasculature directly to restore blood flow. In this study, we developed modular, micro-scale constructs designed to possess robust handling qualities, allow in vitro pre-culture, and promote microvasculature formation. The microbead matrix consisted of an agarose (AG) base to prevent aggregation, combined with cell-adhesive components of fibrinogen (FGN) and/or hydroxyapatite (HA). Microbeads encapsulating a co-culture of human umbilical vein endothelial cells (HUVEC) and fibroblasts were prepared and characterized. Microbeads were generally 80–100 microns in diameter, and the size increased with the addition of FGN and HA. Addition of HA increased the yield of microbeads, as well as the homogeneity of distribution of FGN within the matrix. Cell viability was high in all microbead types. When cell-seeded microbeads were embedded in fibrin hydrogels, HUVEC sprouting and inosculation between neighboring microbeads were observed over seven days. Pre-culture of microbeads for an additional seven days prior to embedding in fibrin resulted in significantly greater HUVEC network length in AG+HA+FGN microbeads, as compared to AG, AG+HA or AG+FGN microbeads. Importantly, composite microbeads resulted in more even and widespread endothelial network formation, relative to control microbeads consisting of pure fibrin. These results demonstrate that AG+HA+FGN microbeads support HUVEC sprouting both within and between adjacent microbeads, and can promote distributed vascularization of an external matrix. Such modular microtissues may have utility in treating ischemic tissue by rapidly re-establishing a microvascular network.

show abstract

Study of fibrinogen adsorption on hydroxyapatite and TiO2 surfaces by electrochemical piezoelectric quartz crystal impedance and FTIR–ATR spectroscopy

Cited by 35 publications

References 35 publications

Influence of surface features of hydroxyapatite on the adsorption of proteins relevant to bone regeneration

Influence of surface features of hydroxyapatite on the adsorption of proteins relevant to bone regeneration

Adsorption of tripeptide RGD on rutile TiO2 nanotopography surface in aqueous solution

Distributed vasculogenesis from modular agarose-hydroxyapatite-fibrinogen microbeads

Contact Info

Product

Resources

About