Study of Enhanced Anti-Inflammatory Potential of Nigella Sativa in Topical Nanoformulation

Abstract

Objective: Formulate a nanocarrier for enhancing the anti-inflammatory activity of thymoquinone (Tq), a major active constituent of Nigella sativa.Methods: Nanoformulation of Tq was developed by low energy emulsification techniques. NanoTqs were pre-screened by different thermodynamic stability tests, followed by in vitro release, zeta potential, viscosity, the transmittance (%), globule size distribution and ex vivo studies. The morphology of the optimized NanoTq was determined by transmission electron microscopy (TEM) which revealed fairly spherical shape and good correlation with particle size distribution study. The formulation used for assessment of the anti-inflammatory potential and permeability enhancement contained mixture of essential oil of Nigella sativa: Capryol 90 (3:7, 10%, v/v), Tween 80 (21.75%, v/v), PEG 400 (7.25%, v/v) and double distilled water (61%, v/v). Results:The in vitro permeation of Tq from optimized formulations was found extremely significant (p<0.001) in comparison to apiTq. The steady state flux (Jss), the permeability coefficient (Kp) and enhancement ratio (Er) of NanoTq gel was determined and compared with apiTq. The comparative anti-inflammatory effects of the optimized formulations NanoTq, apiTq and DicloGel was assessed on the edema in the carrageenaninduced paw model in Wistar rats. Therapeutic potential of NanoTq was found statistically extremely significant (P<0.0001) compared to apiTq and insignificant comparable with standard DicloGel. Storage stability of NanoTq showed insignificant changes in the zeta potential, droplet size and was free from any physical instability. Conclusion:The optimized nano formulation with a lower dose of Tq showed better anti-inflammatory effects, indicating greater absorption capability through the stratum corneum.

Development and Optimization of Methylcellulose-Based Nanoemulgel Loaded with Nigella sativa Oil for Oral Health Management: Quadratic Model Approach

“…The most optimized formulation obtained after 17 runs was further characterized for appearance, globule size distribution, in vitro release properties, and stability profile. PEG 200 surfactant was chosen as an emulsifying agent, which can be used in the concentration of 0.1% to 10% by weight in nanoformulations [ 30 , 31 ]. Here, PEG 200 concentration was kept constant at 5% by weight for all the prepared formulations.…”

Essential Oils and Isolated Terpenes in Nanosystems Designed for Topical Administration: A Review

“…There is a number of good reviews in the literature that list the variety of nanostructured systems and preparation techniques employed in the encapsulation of EO in nanostructures systems [1,2,104], but none focused on terpenes. Among the studies listed in Table 1 and Table 2, the most widely investigated biological activities upon nanoencapsulation of essential oils and isolated terpenes were the anti-inflammatory activity of essential oils and oil resins containing terpenes, such as plai oil [93], Copaifera multijuga oil [89,97], Nigella sativa EO [78], and of the isolated terpenes triptolide [41,65,71,73], sericoside [72], thymol [10] and oleanoic and ursolic acids [53], as well as the wound healing properties of Syzygium aromaticum EO [83], Melaleuca alternifolia EO [91], triterpenes from Centella asiatica extract [85], madecassoside [105], asiaticoside [48], astragaloside IV [59], and hyperforin [11]. Also, the nanoencapsulation of the well-established taxane antineoplastic drugs paclitaxel [14] and docetaxel [13,35] were studied for topical administration.…”

Evaluation of the Protective Effect of Nigella Sativa (Black Cumin) Oil Against Vancomycin-Induced Nephrotoxicity in Rats

“…NS was found to have a renoprotective effect because of its anti-inflammatory and antioxidant properties. This effect is attributed to the active ingredients TQ, carvacol, and others [6]. In the present study, parameters of oxidative stress, MDA and GSH were evaluated in the homogenate of kidney tissue.…”

“…Nigella sativa (NS) is a medicinal plant which is known to have a lot of pharmacological actions, which for most of these actions, antioxidant activity is responsible [6].…”