Fish oil consists of omega-3 fatty acids which play an important role in human
health. Its susceptibility to oxidation causes considerable degradation during
the processing and storage of food products. Accordingly, encapsulation of this
ingredient through freeze drying was studied with the aim of protecting it
against environmental conditions. Gum arabic (GA) was used as the wall material
for fish oil nanoencapsulation where tween 80 was applied as the emulsifier. A
water-in-oil (W/O) emulsion was prepared by sonication, containing 6%
fish oil dispersed in aqueous solutions including 20% and 25%
total wall material. The emulsion was sonicated at 24 kHz for 120 s. The
emulsion was then freeze-dried and the nanocapsules were incorporated into
probiotic fermented milk, with the effects of nanocapsules examined on the milk.
The results showed that the nanoparticles encapsulated with 25% gum
arabic and 4% emulsifier had the highest encapsulation efficiency (EE)
(87.17%) and the lowest surface oil (31.66 mg/100 kg). Using
nanoencapsulated fish oil in fermented milk significantly (p<0.05)
increased the viability of
Lactobacillus plantarum
as well as
eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents. The
fermented milk sample containing fish oil nanoencapsulated with 25% wall
material and 4% emulsifier yielded the greatest probiotic bacterial count
(8.41 Log CFU/mL) and the lowest peroxide value (0.57 mEq/kg). Moreover, this
sample had the highest EPA and DHA contents. Utilizing this nanoencapsulated
fish oil did not adversely affect fermented milk overall acceptance. Therefore,
it can be used for fortification of low fat probiotic fermented milk.