Study of DNA Interaction with Cobalt Ferrite Nanoparticles

Abstract

Interaction of cobalt ferrite nanopowder and nucleic acid was investigated. Superparamagnetic cobalt ferrite nanoparticles (6-12 nm) were prepared by mechanochemical synthesis. Structure of the nanopowder was characterized using X-ray diffraction. It was shown that cobalt ferrite nanoparticles were associated with ssDNA and dsDNA in Tris-buffer resulting in bionanocomposite formation with mass weight relation nanoparticles: DNA 1:(0.083 +/- 0.003) and 1:(0.075 +/- 0.003) respectively. The mechanism of interaction between a DNA and cobalt ferrite nanoparticles was considered basing on the whole set of obtained data: FTIR-spectroscopy, analyzing desorption of DNA from the surface of the particles while changing the chemical content of the medium, and on the modeling interaction of specific biomolecule fragments with surface of a inorganic material. It was supposed that the linkage was based on coordination interaction of the phosphate groups and oxygen atoms heterocyclic bases of DNA with metal ions on the particle surface. These data can be used to design specific magnetic DNA-nanoparticles hybrid structures.

<i>In Vitro</i> Evaluation of the Toxicity of Cobalt Ferrite Nanoparticles in Kidney Cell

“…23 It was reported the interaction between CoFe 2 O 4 -NPs and nucleic acid occurred, and the linkage was based on a coordination interaction of the phosphate groups and the oxygen atoms on the heterocyclic bases of DNA with metal ions on the particle surface. 24 Also, Ahmad et al 10 pointed out the genotoxicity of CoFe 2 O 4 -NPs. Similarly, Colognato et al 25 reported the induction of genotoxicity in human peripheral lymphocytes exposed those CoFe 2 O 4 -NPs.…”

Genotoxicity and carcinogenicity of cobalt-, nickel- and copper-based nanoparticles

“…Cobalt-ferrite nanoparticles (CoFe 2 O 4 ; 6–12 nm) were used to investigate the interaction with nucleic acid (63). The investigators in this study observed that the research data collectively revealed that there was an interaction between CoFe 2 O 4 nanoparticles and nucleic acid.…”

Antibacterial activity of transition metal complexes with a tridentate NNO amoxicillin derived Schiff base. Synthesis and characterization

“…Due to these circumstances, new strategies are required to improve the antibacterial therapy. Among them, one strategy aims at the development of new drugs by re−engineering existing drugs to improve their bioavailability and antibacterial activity …”

“…Transition metals can exhibit a wide variety of coordination properties and reactivity, which can be used to form complex with organic drugs as ligands. Transition metal complexes exhibit unique and interesting properties such as changing oxidation states and the ability to form specific interactions with other biomolecules . Thus the coordination of some metals with antibiotics would prevent development of resistance by microbes, enabling novel mechanisms of action and enhance the antimicrobial property of the antibiotic .…”

“…Among them, one strategy aims at the development of new drugs by re−engineering existing drugs to improve their bioavailability and antibacterial activity. [1,2] Transition metals can exhibit a wide variety of coordination properties and reactivity, which can be used to form complex with organic drugs as ligands. Transition metal complexes exhibit unique and interesting properties such as changing oxidation states and the ability to form specific interactions with other biomolecules.…”