Study of DNA hybridization on polypyrrole grafted with oligonucleotides by photocurrent spectroscopy

Lassalle, N.; Vieil, E.; Correia, J. Pinto; Abrantes, L.M.

doi:10.1016/s0956-5663(01)00133-6

Cited by 29 publications

(23 citation statements)

References 23 publications

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“…Electrically conductive polymers have been used in various biomedical applications including biosensors,1 neural prostheses,2, 3 actuators,4 and controlled release 5. They have also been used to regulate cell functions by applying electrical stimulation (ES) through the conductive polymers, especially for electrically excitable cells such as neuronal or muscle cells 6, 7.…”

Section: Introductionmentioning

confidence: 99%

Electrical regulation of Schwann cells using conductive polypyrrole/chitosan polymers

Huang

Lü

et al. 2009

J Biomedical Materials Res

184

121

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Electrical stimulation (ES) can dramatically enhance neurite outgrowth through conductive polymers and accelerate peripheral nerve regeneration in animal models of nerve injury. Therefore, conductive tissue engineering graft in combination with ES is a potential treatment for neural injuries. Conductive tissue engineering graft can be obtained by seeding Schwann cells on conductive scaffold. However, when ES is applied through the conductive scaffold, the impact of ES on Schwann cells has never been investigated. In this study, a biodegradable conductive composite made of conductive polypyrrole (PPy, 2.5%) and biodegradable chitosan (97.5%) was prepared in order to electrically stimulate Schwann cells. The tolerance of Schwann cells to ES was examined by a cell apoptosis assay. The growth of the cells was characterized using DAPI staining and a MTT assay. mRNA and protein levels of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) in Schwann cells were assayed by RT-PCR and Western blotting, and the amount of NGF and BDNF secreted was determined by an ELISA assay. The results showed that the PPy/chitosan membranes supported cell adhesion, spreading, and proliferation with or without ES. Interestingly, ES applied through the PPy/chitosan composite dramatically enhanced the expression and secretion of NGF and BDNF when compared with control cells without ES. These findings highlight for the first time the possibility of enhancing nerve regeneration in conductive scaffolds through ES-increased neurotrophin secretion.

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Section: Introductionmentioning

confidence: 99%

Electrical regulation of Schwann cells using conductive polypyrrole/chitosan polymers

Huang

Lü

et al. 2009

J Biomedical Materials Res

184

121

View full text Add to dashboard Cite

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“…Electrically conductive polypyrrole (PPy) is biocompatible and has been investigated for various biomedical applications including biosensors,1, 2 neural prostheses,3, 4 actuators,5, 6 and controlled release 7. Another promising application is as a conductive substrate to introduce electrical stimulation (ES) to cells.…”

Section: Introductionmentioning

confidence: 99%

Electrical stimulation enhances viability of human cutaneous fibroblasts on conductive biodegradable substrates

Shi

Rouabhia

Meng

et al. 2007

J Biomedical Materials Res

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Electrically conductive biodegradable polymer membranes were prepared by mixing conductive polypyrrole particles with poly(L-lactic acid) solution followed by solution casting and solvent evaporation. Multi-well electrical cell culture plates were fabricated to host electrically stimulated cell culture and monitor parameters. Human cutaneous fibroblasts were cultured on conductive membranes with or without electrical stimulation (ES). Cell count, MTT, Hoechst staining, and SEM were performed to characterize the cells. The membranes supported the adhesion and proliferation of the fibroblasts in both the presence and absence of ES. In the presence of direct electrical field strength of 100 mV/mm, cell viability on the PPy/PLLA membranes at 2 and 24 h was 2.2- and 4.0-fold (p < 0.05) respectively of that on the same membranes without ES. Direct electrical current ranging from 2.5 to 250 microA/mm had no effect on the viability of cells cultured on the gold-coated Petri dish. Electrical field applied to conductive biodegradable polymer surfaces is therefore an effective approach to upregulate the mitochondrial activity of human skin fibroblasts.

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“…However, DNA sensing based on the photocurrent properties of conducting polymers has not been fully exploited. The initial work on photocurrent spectroscopy as a transduction tool for direct detection of DNA hybridization based on CPs was undertaken by Lassalle et al [90][91][92]. The photocurrent response under white light illumination was analyzed for a polypyrrole copolymer with one monomer unit modified by grafted ODN, and the copolymer exposed to a blank buffer solution, and buffer solutions containing either complementary or non-complementary ODNs (Fig.…”

Section: Photocurrent Spectroscopic Detectionmentioning

confidence: 99%

“…8, monomer 1) and copolymerized this with pyrrole to realize addressable multiple probe immobilization [54,55]. The hybridization was detected by photocurrent spectroscopy [92]. The same group also prepared a biotinylated pyrrole monomer (Fig.…”

Section: Dna Sensors Based On Polypyrrole and Its Derivativesmentioning

confidence: 99%

Conducting polymers for electrochemical DNA sensing

et al. 2009

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Study of DNA hybridization on polypyrrole grafted with oligonucleotides by photocurrent spectroscopy

Cited by 29 publications

References 23 publications

Electrical regulation of Schwann cells using conductive polypyrrole/chitosan polymers

Electrical regulation of Schwann cells using conductive polypyrrole/chitosan polymers

Electrical stimulation enhances viability of human cutaneous fibroblasts on conductive biodegradable substrates

Conducting polymers for electrochemical DNA sensing

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