Study of chromium, selenium and bromine concentrations in blood serum of patients with parenteral nutrition treatment using total reflection X-ray fluorescence analysis

Pierzak, Monika; Kubala‐Kukuś, A.; Banaś, D.; Stabrawa, I.; Wudarczyk-Moćko, J.; Głuszek, Stanisław

doi:10.1371/journal.pone.0243492

Cited by 10 publications

(7 citation statements)

References 20 publications

(29 reference statements)

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“…There is no well-defined reference interval for bromine. The population reference range derived from data from a large laboratory (2.5th–97.5th percentiles) has been established at 0.9–7.3 mg/L [ 19 ], but most literature reports values between 3 and 7 mg/L [ 20 , 21 , 22 , 23 , 24 , 25 ]. It can therefore be concluded that serum bromine levels were normal in the control group and significantly decreased in the patient group.…”

Section: Resultsmentioning

confidence: 99%

Serum Iodine and Bromine in Chronic Hemodialysis Patients—An Observational Study in a Cohort of Portuguese Patients

Novakova

Bonev

Duro

et al. 2023

Toxics

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Background: Patients on chronic hemodialysis therapy are at high risk of disturbances in trace element status due to both the underlying disease and the hemodialysis process itself. Data on iodine and bromine levels in these patients are scarce. Methods: Using an ICP-MS analytical procedure, serum iodine and bromine levels were determined in a cohort (n = 57) of end-stage renal disease patients on chronic hemodialysis. The results were compared with those of a control group (n = 59). Results: Hemodialysis patients presented serum iodine levels within the normal range, slightly lower than in controls, but without reaching a statistically significant difference (67.6 ± 17.1 µg/L vs. 72.2 ± 14.8 µg/L; p = 0.1252). In contrast, serum bromine levels were much lower in patients (1086 ± 244 µg/L vs. 4137 ± 770 µg/L; p < 0.0001), at values only about 26% of the values observed in controls. Conclusions: Hemodialysis patients had normal serum iodine levels, but highly decreased serum bromine levels. The clinical significance of this finding requires further investigation, but it may be associated with sleep disturbances and fatigue that affect hemodialysis patients.

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Section: Resultsmentioning

confidence: 99%

Serum Iodine and Bromine in Chronic Hemodialysis Patients—An Observational Study in a Cohort of Portuguese Patients

Novakova

Bonev

Duro

et al. 2023

Toxics

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“…The detection limits of WDXRF compared to the ICP-OES were calculated (Table 2), and the WDXRF was trustworthy to be used in daily diagnosis, particularly in detecting a high concentration of these elements, which is usually associated with animal toxicosis [12,13]. The WDXRF technique has been extensively used for geological, metallic, and other sample analyses but less for biological purposes, where its usage has been expanded and grown substantially [11,[19][20]. The WDXRF has been lowly used for diagnostics in veterinary medicine, but fortunately, this study showed that it is possible to use this technique in this area successfully.…”

Section: Resultsmentioning

confidence: 99%

Quantitative analysis of minor and trace elements in blood serum of dairy cattle (Bos taurus) by WDXRF

et al. 2022

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Assessment of heavy metal content in biological fluids is essential to determine human and animal health as a result of exposure to contaminated environments, food, and water. WDXRF is a non-destructive method that involves no chemical treatments. It is possible to perform fast chemical analysis without producing hazardous waste and uses a small sample volume (± 50 mL), which is useful to veterinary usage. Therefore, the aim of this work was to implement an alternative method for minor and trace elements in blood serum samples of dairy cattle (Bos taurus) using WDXRF. It was implemented for the determination of Al, Cr, Co, Fe, Cu, As, and Hg, and the method was evaluated by comparing WDXRF and ICP-OES results of a set of 14 blood serum samples of dairy cattle using principal component analysis (PCA). Results of WDXRF and ICP-OES were statistically equivalent to all analyzed elements, except for Fe and Al, which were still comparable. WDXRF proved to be a suitable alternative methodology for heavy metal detection in animal blood serum samples due to the low sample volume. The results of this study enable a new and fast method for veterinary internal medicine, which requires a technique that quickly detects animals with a high concentration of toxic elements to assure the correct protocols to prevent the animal's death.

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“…The study's purpose was to determine the concentration levels of several elements (P, S, Cl, K, Ca, Cr, Fe, Cu, Zn, Se, Br, Rb, Pb) in human serum and to define their reference values. More recently, Pierzak et al [ 52 ] used TXRF to determine chromium, selenium and bromine concentrations in blood serum samples of 50 patients with parenteral nutrition treatment. For comparison purposes, serum samples of 50 patients without nutritional disorders were analysed and treated as the control group.…”

Section: Bioanalytical Applicationmentioning

confidence: 99%

TXRF spectrometry in the bioanalytical sciences: A brief review

Fernández-Ruiz

2021

X-Ray Spectrometry

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Funding informationUniversidad Aut onoma de Madrid This work describes the basic principles of Total-reflection X-Ray Fluorescence (TXRF) spectrometry and some of its most outstanding applications for investigating metal traces, mainly in biological and biomedical samples. TXRF is an analytical technique that combines the versatility of elemental analysis by X-ray energy dispersion with a low detection limit in the order of picograms of absolute mass or a few ppb (μg L À1 ) as concentration. The microanalytical capacity of TXRF allows the study of small sample quantities. The TXRF can perform qualitative and mass ratio analyses of the elements present in a sample of only a few hundred nanograms. These properties are of great importance in the research of metal traces in biological systems, as they allow fast, precise, and accurate characterisation of the elemental fingerprint at trace level. Its successful application in studies of the coordination kinetics of new platinumbased antitumor drugs with DNA, its application in the study profile of the metal in healthy and cancerous human tissues, or even its application in the study of the processes of metal diffusion through cell membranes are just a few examples of TXRF capabilities in the biomedical sciences. This brief review's main objective is to provide an overview of the physical principles and possibilities of TXRF spectrometry. It also highlights some of the most outstanding applications that have been developed so far, mainly in the biological field, so that the reader can evaluate their potential applications.

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Study of chromium, selenium and bromine concentrations in blood serum of patients with parenteral nutrition treatment using total reflection X-ray fluorescence analysis

Cited by 10 publications

References 20 publications

Serum Iodine and Bromine in Chronic Hemodialysis Patients—An Observational Study in a Cohort of Portuguese Patients

Serum Iodine and Bromine in Chronic Hemodialysis Patients—An Observational Study in a Cohort of Portuguese Patients

Quantitative analysis of minor and trace elements in blood serum of dairy cattle (Bos taurus) by WDXRF

TXRF spectrometry in the bioanalytical sciences: A brief review

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