STUDY OF BIODIESEL PRODUCTION FROM OILSEED PLANTS I. EVALUATION OF THE GENETIC SIMILARITY OF SOME Brassica Napus CULTIVARS USING RAPD MARKERS

Abstract

Rapeseed (Brassica napus) is an important oilseed crop worldwide. In order to get the genetic similarity present in a set of oilseed rape cultivars, 32 cultivars have been analyzed at the DNA level by Random Amplified Polymorphic (RAPD) technique. RAPD analysis was performed with 8 decamer primers to produce reliable polymorphic DNA bands ranging in molecular weight from 238 -961 bp. A total of 72 polymorphic bands from 96 reproducible bands were obtained. Based on these data, genetic similarity (GS) was estimated between 0.608 ("Uspekh" versus "Libritta") and 0.731 ("Libritta" versus "Uspekh"). UPGMA cluster analysis carried out on these data indicates a good fit of the respective clusters to genetic similarity data. The correlation of cluster analyses to pedigree information and the impact on parental genotype selection has been taken into discussion.

Evaluation of genetic diversity in Chinese kale (Brassica oleracea L. var. alboglabra Bailey) by using rapid amplified polymorphic DNA and sequence-related amplified polymorphism markers

“…Among them, random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) are more commonly used markers. Molecular markers such as RAPD were previously used to assess genetic diversity among the species of Chinese kale (Matsui et al, 2002), cabbage (Cansian and Echeverrigaray, 2000;Koutita et al, 2005), kale (Okumus and Balkaya, 2007), cauliflower (Astarini et al, 2005(Astarini et al, , 2006, broccoli , mustard (Rabbani et al, 1998;Teklewold and Becker, 2006;Khan et al, 2008Khan et al, , 2011, non-heading Chinese cabbage (Han and Cheng, 2010), and rape (Riaz et al, 2001;Hu et al, 2003;Gecaite et al, 2009;Leonte et al, 2010). Besides, SRAP markers have been used for genetic diversity analysis of different species of Brassica (Li and Quiros, 2001;Wu et al, 2009;Han and Cheng, 2010;Jing et al, 2011;Yu et al, 2011).…”