Study of bilineage differentiation of human-bone-marrow-derived mesenchymal stem cells in oxidized sodium alginate/N-succinyl chitosan hydrogels and synergistic effects of RGD modification and low-intensity pulsed ultrasound

Dextran-based hydrogel formed by thiol-Michael addition reaction for 3D cell encapsulation

“…ALP activity was very low at the initial culture time point (day 1) and significant increase was detected from day 3, reaching a peak at day 9, and then, the ALP activity decreased gradually (Fig. 5E), which was consistent with the previous report [55,56], demonstrating the osteogenesis process. As a contrast, the increase of ALP expression of BMSC cultured in normal medium (control group) is not significant.…”

“…When cultured in osteogenic induction medium, BMSCs can differentiate into osteoblasts, and alkaline phosphatase (ALP) plays an important role during the calcified tissue formation process. As described in previously reported literatures, ALP expression of BMSC cultured in osteogenic induction medium increased at the initial culture time and then started to decrease after culturing for about 10-14 days [55,56]. To quantify the differentiation ability of BMSCs encapsulated in the Dex-l-DTT hydrogel, ALP activity was detected at day 1, 3, 5, 7, 9, and 14.…”

Preparation and characterization of composite hydrogel beads based on sodium alginate

“…In this work, ADH was selected as a bifunctional cross-linking molecule without employing any extraneous chemical cross-linking agents, which can develop chemical cross-linking reaction with amino functions via Schiff's base linkage (see Fig. 3) [25,26]. The experimental mechanism of the formation of three-dimensional network hydrogel is as follows: on one hand, SA molecules are polymerized by 1,4-glycosidic bond from b-D-mannuronic acid (M unit) and a-L-guluronic acid (G unit)…”

Smart Matrices for Distal Lung Tissue Engineering

“…14 In spite of obvious shortcomings, these advances have bestowed the capacity to modulate cellular behavior. 15 Specific examples include the enhancement of angiogenic processes using FGF-2/VEGF-sequestering heparinized PEG hydrogels 16 and improved adhesion and differentiation of human fetal neural precursor cells on IKVAV peptide-containing poly-HEMA hydrogels. 17 Another important potential function of biomimetic matrices is the control of ECM assembly at the cell-biomaterial interface, as has been demonstrated by incorporation of an osteocalcin functional domain that enhanced incorporation of hydroxyapatite in engineered bone constructs.…”