Study of BECKMAN COULTER LH 750 CPD parameters in subclinical Vitamin B12 deficiency

Hawaldar, Ranjana; Sodani, Sadhna

doi:10.5958/2394-6792.2016.00131.9

Cited by 1 publication

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“…[3] However studies have shown that MCV alone as screening test may be unreliable due to concomitant iron deficiency anemia, anemia of chronic disease or thalassemia carrier state. [3,11] A systematic review showed that the MCV is far from perfect in terms of detecting or excluding B12 deficiency, both in anaemic and non-anaemic patients. Even in retrospective series of patients with pernicious anaemia, the sensitivity of the MCV for B12 deficiency was far from perfect, with a summary estimate of 77%.…”

Section: Discussionmentioning

confidence: 99%

The Role of Volume, Conductivity, Scatter Changes of Neutrophils and Monocytes in Diagnosis of Megaloblastic Anemia

Adiga¹,

Saha²,

Rajendran³

et al. 2020

Ann of Pathol and Lab Med

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Background: Megaloblastic anemias are macrocytic normochromic anemia with mean corpuscular volume (MCV) of 100 fl-140 fl and caused by deficiency of either cobalamin (vitamin B12) or folate. However, increased MCV is not specific for megaloblastic anemia, nor is Vitamin B12 assay by chemiluminescence. We undertook this study to evaluate the possible role of Volume, Conductivity and Scatter (VCS) of WBCs derived from standard hematology analyzer to indicate megaloblastic anemia. Methods: We performed a case control study comparing data of 60 patients with low serum vitamin B12 or folate levels with 60 healthy volunteers. Comparison of the volume, conductivity and scatter parameters for neutrophils and monocytes of cases and control were done. Result: The mean neutrophil volume of cases (158.37±18.13fl) was significantly higher (p= 0.0001) compared to controls (141.26±4.22fl). Similarly, mean monocyte volume of cases (183.34±16.90fl) was significantly (p=0.0001) higher compared to controls (166.55±8.66fl). The difference in the mean conductivity of both neutrophils and monocytes between cases and controls were insignificant (p=0.43). Conclusion: Our study suggests analysis of VCS parameters for neutrophils and monocytes was a simple and objective method that substantiates the existence of subclinical deficiency of vitamin B 12 and folate with fair degree of certainty.

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Section: Discussionmentioning

confidence: 99%