Study of Apolipoprotein E Polymorphism in Normal Healthy Controls from Northern India

Chhabra, S.; Agarwal, D. P.; Vasisht, Suman; Luthra, Kalpana; Narang, Rajeev; Manchanda, Smita; Srivastava, Lalit M.; Das, Nibhriti

doi:10.1155/2000/970498

Cited by 6 publications

(7 citation statements)

References 5 publications

(10 reference statements)

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“…A significantly high frequency of the apo ε4 allele was observed in the stroke patients as compared with the controls (p < 0.005) (Table 2). The frequency of the ε4 allele in the control subjects of this study (11%) is similar to that reported in a recent study conducted by us in normal healthy controls of this population (9%) (29).…”

Section: Discussionsupporting

confidence: 91%

Apolipoprotein E gene polymorphism in cerebrovascular disease: a case–controlstudy

et al. 2002

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The association between apolipoprotein E (apo E) polymorphism and stroke has been controversial. So far there are no studies reported on the polymorphism of apolipoprotein E in cerebrovascular diseases in the Asian Indians. A blinded case-control study was therefore undertaken and the apo E genotypes and lipid profile of a total of 120 subjects (63 stroke patients and 57 healthy controls) were done. The frequency distribution of apo E alleles and genotypes were assessed and their relation with the occurrence of stroke in Asian Indian subjects was determined. A significantly high frequency of apo epsilon4 allele (30%) was observed in the stroke patients than the controls (11%) (p < 0.005), and patients with epsilon4 allele had a fourfold higher odds to develop stroke OR (95%CI) 4.2 (1.8-10.1) (p < 0.005). On multivariate analysis, after adjusting for age, triglycerides and hypertension, the association of epsilon4 allele with stroke was found to be no longer statistically significant, OR (95%CI) 1.2 (0.4-4.5) (p = NS). On multiple logistic regression analysis age, OR (95%CI) 1.1 (1.1-1.2) (p < 0.001), and hypertension OR (95%CI) 15.1 (2.6-89.1) (p < 0.005) were found to be independent risk factors for development of stroke. This is the first report to have examined the association of apo E gene polymorphism with stroke in the Asian Indians. This study suggests that apo epsilon4 allele, triglycerides, age and hypertension are the predictors for stroke development.

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Section: Discussionsupporting

confidence: 91%

Apolipoprotein E gene polymorphism in cerebrovascular disease: a case–controlstudy

et al. 2002

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“…The homozygous E3 genotype was most common in controls (77.8%), followed by E3/E4 (14.7%) and E2/E3 (6.5%), respectively, which is similar to results reported from studies in India. [ 12 13 ] In contrary to the other population of the world, the E4/E4 genotype was absent in our study cohort. This may be due to the small number of sample size.…”

Section: Discussioncontrasting

confidence: 90%

“…This may be due to the small number of sample size. Similar results were drawn from a study from Northern India,[ 12 ] where they concluded that homozygous E4 genotype was rare in the studied population, and in another study, it was found to be absent in Koch and Maria Gond population of India. [ 13 ]…”

Section: Discussionsupporting

confidence: 88%

Association of APOE gene polymorphism with stroke patients from rural Eastern India

Ganaie

Biswas

Bhattacharya

et al. 2020

Ann Indian Acad Neurol

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Context: Studies from the different ethnic regions of the world have reported variable results on association of APOE gene polymorphism in stroke. Aim: The aim of this study is to find out the possible association of APOE polymorphism in stroke patients in ethnic Bengali population. Settings and Design: A prospective case–control study was undertaken in the Department of Neurology, Burdwan Medical College, Burdwan, West Bengal, India, over a period of 3 years. Methods: We collected 10 ml venous blood samples from 148 clinically and radiologically diagnosed acute stroke patients (80 of ischemic stroke and 68 of intracerebral hemorrhage) and consecutive 108 ethnic age- and sex-matched controls, in ethylenediaminetetraacetic acid vials after informed written consent. Genomic DNA was prepared at S.N. Pradhan Centre of Neurosciences, University of Calcutta, Kolkata, India. Exotic single-nucleotide polymorphisms (rs429358, rs 7412) were analyzed by polymerase chain reaction-restriction fragment length polymorphism for genotype of APOE. Results: The frequencies of different APOE allele among 80 ischemic stroke patients were 5.6% ( n = 9) for E2, 75.68% ( n = 121) for E3, and 18.7% ( n = 30) for E4. The E3 allele is significantly over-represented ( P = 0.004) in controls compared to the patients (88% in controls vs 75.6% ischemic stroke patients and 80% hemorrhagic patients). A significantly high frequency of APOE4 allele was observed in ischemic (18.7%) and hemorrhagic patients (11%) compared to controls (8%). The E4 allele plays a major risk for developing ischemic stroke [odds ratio (OR) = 2.744; 95% confidence interval (CI): 1.43–5.10] and E3 plays a protective role for hemorrhagic stroke (OR = 0.53; 95% CI: 0.29–0.96), while E4 allele plays a nonsignificant ( P = 0.31) increase in trend in hemorrhage stroke (OR = 1.4). Conclusions: There is significant association of APOE gene polymorphism in stroke patients of ethnic Bengali population. The E4 allele increases significant risk for development of ischemic strokes, and it also plays nonsignificant increase in trend in hemorrhagic strokes.

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“…31 Based on these observations and the data of their study that 89% of normal healthy individuals of North India have the genotypes E3/E3, E3/E4 and E4/E4, Chhabra et al proposed that a large proportion of normal individuals in North India are genetically predisposed to CHD. 17 In addition, multiple lipoprotein abnormalities (high levels of LDL-C and very-lowdensity lipoprotein triglycerides) and apo E genotype, were suggested as the best model for predicting CHD. 32 Apo E genotype can in£uence the absorption 34 and clearance of dietary lipids; 35 however, apo E association with blood lipid concentrations has been variously reported as both present 37,38 and absent, 12,15,36 as in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…16 In healthy Asian Indians in North India, E3/E3 (72¢1%) was the commonest genotype, followed by the E3/E4 genotype (17%). 17 Furthermore the E3/E3 genotype was associated with lower levels of high-density lipoprotein cholesterol (HDL-C) in CHD patients from the same population. 18 However, there are no reports investigating apo E gene polymorphisms in Asian Indian patients with premature MI.…”

Section: Introductionmentioning

confidence: 99%

Apolipoprotein E gene polymorphisms in patients with premature myocardial infarction: a case-controlled study in Asian Indians in North India

et al. 2003

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Study of Apolipoprotein E Polymorphism in Normal Healthy Controls from Northern India

Cited by 6 publications

References 5 publications

Apolipoprotein E gene polymorphism in cerebrovascular disease: a case–controlstudy

Apolipoprotein E gene polymorphism in cerebrovascular disease: a case–controlstudy

Association of APOE gene polymorphism with stroke patients from rural Eastern India

Apolipoprotein E gene polymorphisms in patients with premature myocardial infarction: a case-controlled study in Asian Indians in North India

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