Study of antiapoptotic effect of a protein isolated from Megalopyge albicolis (Lepidoptera: Megalopygidae) hemolymph

Mazzoni, Marina Katia Ferreira; Carvalho, Nathalia Delazeri de; Rofatto, Henrique K.; Moraes, Roberto Enrique Pinto; Mendonça, Ronaldo Zucatelli

doi:10.1186/1753-6561-8-s4-p148

Cited by 2 publications

(1 citation statement)

References 3 publications

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“…Knowledge of cell death mechanisms can lead to important strategies in the control of various physiological processes. We previously identified substances with potent anti-apoptotic action in the hemolymph of caterpillars of the Saturnidade family (Souza et al, 2005) and Megalopyge (Mazzoni et al, 2014). So, due to the importance of studies on cell death, the objective of this research is the identification, isolation, and purification of substances with anti-apoptotic action in the hemolymph of caterpillars of the Megalopygidae family.…”

Section: Introductionmentioning

confidence: 99%

Proteins with anti-apoptotic action in the hemolymph of caterpillars of the Megalopygidae family acts by maintaining the structure of the cellular cytoskeleton

Carvalho

Rofatto

Villar

et al. 2023

Preprint

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Brazil has a very large biological variety, which is an almost inexhaustible source of substances of pharmacological and biotechnological interest. Several studies have demonstrated the presence of bioactive peptides in insect hemolymph and their potential use as therapeutic agents. However, few data are available regarding molecules extracted from insects with anti-apoptotic action. The objective of this work was to identify and isolate proteins from the hemolymph of caterpillars of the Megalopygidae family with pharmacological and biotechnological interest. Two species of this family were studied, Podalia sp and Megalopyge albicolis. Cytotoxicity tests on Vero and Sf-9 cells revealed that the hemolymph of both caterpillars were cytotoxic only at concentrations greater than 5%v/v. In the anti-apoptotic activity assays, it was verified that the supplementation of cell cultures with only 1% of hemolymph v/v is sufficient to inhibit cell death by apoptosis induced by different inducers such as terbutyl, actinomycin D, hydrogen peroxide or even by nutrient depletion. For this study, cells were staining with trypan blue, crystal violet and fluorescent markers to cytoskeleton (actin and tubulin), mitochondria membrane electric potential (JC-1) and apoptosis marker (acridine orange and ethidium). The protein responsible for anti-apoptotic action was isolated through gel filtration chromatography, using an AKTA purifier high-resolution liquid chromatography system. The hemolymph was fractionated into 3 pools for Podalia sp and 6 pools for M. abicolis. In the antiapoptotic tests, semi purified hemolymph from both caterpillars showed anti-apoptotic effect in VERO and SF-9 cells, pre-treated with only 1% v/v of hemolymph and induced to death by different and apoptotic inductors. Was observed that the molecule with anti-apoptotic effect are present in pool 3 in both hemolymphs. This protector effect blocked and attenuated the disruption of the cytoskeleton (actin filaments), being that the protective effect also was observed on the integrity of the mitochondrial membrane of SF-9 cells pre-treated with both hemolymphs and treated with the apoptosis inducer Terbutil at concentrations of 25 to 100uM.

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Section: Introductionmentioning

confidence: 99%

Proteins with anti-apoptotic action in the hemolymph of caterpillars of the Megalopygidae family acts by maintaining the structure of the cellular cytoskeleton

Carvalho

Rofatto

Villar

et al. 2023

Preprint

Self Cite

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In vitro antitumor, pro-inflammatory, and pro-coagulant activities of Megalopyge opercularis J.E. Smith hemolymph and spine venom

Orozco-Flores

Valadéz-Lira

Covarrubias-Cárdenas

et al. 2020

Sci Rep

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Contact with stinging spines venom from several Lepidoptera larvae may result in skin lesions. In Mexico, envenomation outbreaks caused by Megalopyge opercularis were reported between 2015 and 2016. The aim of this study was to identify the venomous caterpillars in Nuevo Leon, Mexico and evaluate several biological activities of their hemolymph (HEV) and spine setae (SSV) venoms. M. opercularis was identified by cytochrome oxidase subunit (COI) designed primers. HEV and SSV extracts cytotoxic activity was assessed on the L5178Y-R lymphoma cell line. For apoptotic cells number and apoptosis, cells were stained with acridine orange/ethidium bromide and validated by DNA fragmentation. Human peripheral blood mononuclear cells (hPBMC) cytokine response to the extracts was measured by the cytometric bead array assay. Extracts effect on pro-coagulation activity on human plasma was also evaluated. HEV and SSV extracts significantly inhibited (p < 0.01) up to 63% L5178Y-R tumor cell growth at 125–500 µg/mL, as compared with 43% of Vincristine. About 79% extracts-treated tumor cells death was caused by apoptosis. Extracts stimulated (p < 0.01) up to 60% proliferation of resident murine lymphocytes, upregulated IL-1β, IL-6, IL-8, and TNF-α production by hPBMC, and showed potent pro-coagulant effects. The pharmacological relevance of these venoms is discussed.

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Study of antiapoptotic effect of a protein isolated from Megalopyge albicolis (Lepidoptera: Megalopygidae) hemolymph

Cited by 2 publications

References 3 publications

Proteins with anti-apoptotic action in the hemolymph of caterpillars of the Megalopygidae family acts by maintaining the structure of the cellular cytoskeleton

Proteins with anti-apoptotic action in the hemolymph of caterpillars of the Megalopygidae family acts by maintaining the structure of the cellular cytoskeleton

In vitro antitumor, pro-inflammatory, and pro-coagulant activities of Megalopyge opercularis J.E. Smith hemolymph and spine venom

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