Study of Anti-Inflammatory Activity of Fatsiphloginum™ (Fatsia japonica) and a New Purified Triterpene-Rich Extract of Saponins (PS-551) in Experimental Model of Arthritis

Abstract

In this study, two extracts from Fatsia japonica-Fatsiphloginum™ (extract of triterpene glycosides containing 45-50 % of fatsiosides (FS)) and purified triterpene-rich extract of saponins with code name PS-551 (PS) were administered in combination with methotrexate (MTX) and in monotherapy to rats suffering adjuvant arthritis (AA). The anti-inflammatory activities of extracts were evaluated as monotherapies in comparison with untreated AA. PS administered in higher dose showed on day 28 effective decrease of hind paw volume (HPV), decreased activity of gamma-glutamyl transferase (GGT) in joints, and also interleukin-17A was decreased significantly on day 14. The higher dose of PS was more effective than both doses of FS. Further, we evaluated the higher doses of PS and FS in combination with MTX. PS improved the effect of MTX in combination more effective than FS (HPV, body weight and activity of GGT in joint). However, FS was more effective in reducing the level of IL-17A on day 14 and activity of GGT in spleen than PS. In conclusion, our study showed that generally FS has higher anti-arthritic activity comparing to PS. Thus, the novel combination of Fatsiphloginum™ and methotrexate could be interesting for future clinical studies in patients suffering auto-immune diseases.

Bioflavonoid Robinin from Astragalus falcatus Lam. Mildly Improves the Effect of Metothrexate in Rats with Adjuvant Arthritis

“…However, RB in combination with MTX decreased the IL-17A levels in plasma on days 14 and 21 significantly (Figure 5). Our previous results with fatsiphloginum, especially in combination with MTX, are comparable, with results from this study [41]. The anti-inflammatory activity of robinin could be explained also via its metabolites.…”

“…Earlier findings suggested that major clinical markers, HPV and body weight gain, are remarkably worsened due to inflammation and were effectively diminished by administration of compounds with anti-oxidative and/or anti-inflammatory properties [38][39][40]. The protective effect of MTX against AA development agrees with previously reported results [23,28,37,40,41] as well as with changes of HPV shown in Figure 3. Combination of RB with MTX did not improve these protective effect of MTX (Figures 2 and 3).…”

Combination Therapy of Carnosic Acid and Methotrexate Effectively Suppressed the Inflammatory Markers and Oxidative Stress in Experimental Arthritis

“…The activity of GGT is considered a reliable biochemical merit for inflammation and OS; its increased action in organs such as the spleen and joints is believed to be a good indicator of AA development at the systemic and local level [ 60 ].…”

Effect of Saffron Extract, Astaxanthin, and Carnosic Acid on the Levels of Matrix Metalloproteinase-9 and on Body Weight Changes in Arthritis Experiments

“…This combination has shown better results than M and losartan alone (Refaat et al, 2013). In our previous experiments we focused on combination therapy of M with natural substances and extracts, which has shown significant improvement of several inflammatory markers and biometrical parameters (Tsiklauri et al, 2019(Tsiklauri et al, , 2021.…”