Study of Andes virus entry and neutralization using a pseudovirion system

Ray, Neelanjana; Whidby, Jillian; Stewart, Shaun; Hooper, Jay W.; Bertolotti-Ciarlet, Andrea

doi:10.1016/j.jviromet.2009.11.004

Cited by 43 publications

(34 citation statements)

References 38 publications

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“…This pH was found to be slightly lower than reported previously for Hantaan virus (pH~6.3; Arikawa et al, 1985). For ANDV it has been recently reported that Gn/Gc incorporated into pseudotyped particles suffer irreversible conformational changes at pH~6.0 and below (Ray et al, 2010). Our data indicate that the pH at which ANDV fusion occurs is more similar to that of phlebo-and orthobunyaviruses (pH~5.4-5.8;Hacker & Hardy, 1997;Lozach et al, 2010), indicating that ANDV may correspond to late-penetrating viruses which escape from maturing endosomes or even later compartments.…”

Section: Discussionmentioning

confidence: 52%

Aromatic and polar residues spanning the candidate fusion peptide of the Andes virus Gc protein are essential for membrane fusion and infection

Cifuentes-Muñoz

Barriga

Valenzuela

et al. 2010

Journal of General Virology

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Hantaviruses infect human cells through cell attachment and subsequent fusion of viral and cellular membranes at low pH. This largely unknown entry process is mediated by the Gn and Gc glycoproteins, anchored at the viral envelope membrane. Performing bioinformatic analysis and peptide-liposome-binding assays we suggested in a former report that Gc of Andes virus (ANDV) and other hantaviruses corresponds to the viral fusion protein sharing characteristics with class II fusion proteins. To gain insights into the fusion protein of hantaviruses, residues within the previously predicted fusion peptide of ANDV Gc were substituted and mutant proteins tested in fusion and infection assays. To ensure proper folding of mutant proteins, they were first characterized for trafficking to the plasma membrane and incorporation on to ANDV Gn/Gc-pseudotyped lentiviral particles. Cell attachment of these particles was assessed using a newly developed binding assay and their subsequent entry properties determined by FACS analysis of transduced cells expressing the GFP reporter gene. Furthermore, a three-colour-based cell-cell fusion assay of ANDV Gn/Gc expressing cells was performed. The results indicate an essential role of conserved Gc residues W115 and N118 in membrane fusion. Conversely, substitutions of the non-conserved Gc residue G116 did not considerably affect fusion and infection. Altogether, the findings are fully consistent with our earlier prediction suggesting Gc residues 115-121 as an internal fusion peptide and further emphasize the importance of aromatic and polar residues in hantavirus-cell membrane fusion.

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Section: Discussionmentioning

confidence: 52%

Aromatic and polar residues spanning the candidate fusion peptide of the Andes virus Gc protein are essential for membrane fusion and infection

Cifuentes-Muñoz

Barriga

Valenzuela

et al. 2010

Journal of General Virology

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“…[17][18][19] A PsVNA measured neutralizing antibody responses to Ebola GP (US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD). 16,[20][21][22][23][24] This assay is based on non-replicating VSV expressing luciferase reporter protein particles pseudotyped with ZEBOV envelope glycoproteins. 20 The assay determined the highest serum dilutions causing 50% (PsVNA50) and 80% (PsVNA80) inhibition of virus cell entry and expression of luciferase.…”

Section: Discussionmentioning

confidence: 99%

Assessing the safety and immunogenicity of recombinant vesicular stomatitis virus Ebola vaccine in healthy adults: a randomized clinical trial

ElSherif

Brown

MacKinnon‐Cameron

et al. 2017

CMAJ

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“…1) represents a unique attenuated replication-competent VSV construct that expresses a glycoprotein from a bunyavirus, although replication-incompetent VSV particles have previously been pseudotyped with glycoproteins from "Old World" (35,49) and "New World" (54,60) hantaviruses. Bunyaviruses are generally believed to mature and bud from the Golgi apparatus, including the incorporation of the glycoproteins into virus particles (63).…”

Section: Discussionmentioning

confidence: 99%

Vesicular Stomatitis Virus-Based Vaccine Protects Hamsters against Lethal Challenge with Andes Virus

Brown

Safronetz

Marzi

et al. 2011

J Virol

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Andes virus (ANDV) is a highly pathogenic South American hantavirus that causes hantavirus pulmonary syndrome (HPS).A high case fatality rate, the potential for human-to-human transmission, the capacity to infect via aerosolization, and the absence of effective therapies make it imperative that a safe, fast-acting, and effective ANDV vaccine be developed. We generated and characterized a recombinant vesicular stomatitis virus (VSV) vector expressing the ANDV surface glycoprotein precursor (VSV⌬G/ANDVGPC) as a possible vaccine candidate and tested its efficacy in the only lethal-disease animal model of HPS. Syrian hamsters immunized with a single injection of VSV⌬G/ANDVGPC were fully protected against disease when challenged at 28, 14, 7, or 3 days postimmunization with a lethal dose of ANDV; however, the mechanism of protection seems to differ depending on when the immunization occurs. At 28 days postimmunization, a lack of detectable ANDV RNA in lung, liver, and blood tissue samples, as well as a lack of seroconversion to the ANDV nucleocapsidprotein in nearly all animals, suggested largely sterile immunity. The vaccine was able to generate high levels of neutralizing anti-ANDV G N /G C antibodies, which seem to play a role as a mechanism of vaccine protection. Administration of the vaccine at 7 or 3 days before challenge also resulted in full protection but with no specific neutralizing humoral immune response, suggesting a possible role of innate responses in protection against challenge virus replication. Administration of the vaccine 24 h postchallenge was successful in protecting 90% of hamsters and again suggested the induction of a potent antiviral state by the recombinant vector as a potential mechanism. Overall, our data suggest the potential for the use of the VSV platform as a fast-acting and effective prophylaxis/postexposure treatment against lethal hantavirus infections.

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Study of Andes virus entry and neutralization using a pseudovirion system

Cited by 43 publications

References 38 publications

Aromatic and polar residues spanning the candidate fusion peptide of the Andes virus Gc protein are essential for membrane fusion and infection

Aromatic and polar residues spanning the candidate fusion peptide of the Andes virus Gc protein are essential for membrane fusion and infection

Assessing the safety and immunogenicity of recombinant vesicular stomatitis virus Ebola vaccine in healthy adults: a randomized clinical trial

Vesicular Stomatitis Virus-Based Vaccine Protects Hamsters against Lethal Challenge with Andes Virus

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