Study of agarose aggregate formation through rheological and DLS analyses

Queiroz, Rogério Pereira de; Hora, Luiz Felipe da; Aum, Pedro Tupã Pandava

doi:10.1007/s00396-020-04698-1

Cited by 2 publications

(1 citation statement)

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“…At high temperatures, agarose exists in the form of random coils in aqueous solutions [ 45 ]. As the temperature decreases to the gelation point, the agarose helices are associated with long fiber-like aggregates that eventually form a percolating network during the sol–gel transition [ 46 , 47 ]. After gelation, the hydrogels remain stable at 37 °C and there is no need to introduce other cross-linking agents.…”

Section: Introductionmentioning

confidence: 99%

Effect of Hydrogel Stiffness on Chemoresistance of Breast Cancer Cells in 3D Culture

Zeng,

Chen,

Yoshitomi

et al. 2024

Gels

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Chemotherapy is one of the most common strategies for cancer treatment, whereas drug resistance reduces the efficiency of chemotherapy and leads to treatment failure. The mechanism of emerging chemoresistance is complex and the effect of extracellular matrix (ECM) surrounding cells may contribute to drug resistance. Although it is well known that ECM plays an important role in orchestrating cell functions, it remains exclusive how ECM stiffness affects drug resistance. In this study, we prepared agarose hydrogels of different stiffnesses to investigate the effect of hydrogel stiffness on the chemoresistance of breast cancer cells to doxorubicin (DOX). Agarose hydrogels with a stiffness range of 1.5 kPa to 112.3 kPa were prepared and used to encapsulate breast cancer cells for a three-dimensional culture with different concentrations of DOX. The viability of the cells cultured in the hydrogels was dependent on both DOX concentration and hydrogel stiffness. Cell viability decreased with DOX concentration when the cells were cultured in the same stiffness hydrogels. When DOX concentration was the same, breast cancer cells showed higher viability in high-stiffness hydrogels than they did in low-stiffness hydrogels. Furthermore, the expression of P-glycoprotein mRNA in high-stiffness hydrogels was higher than that in low-stiffness hydrogels. The results suggested that hydrogel stiffness could affect the resistance of breast cancer cells to DOX by regulating the expression of chemoresistance-related genes.

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Section: Introductionmentioning

confidence: 99%