Study of Acute and Chronic Toxicity of &amp;quot;Butaselmevit&amp;quot; on Laboratory Animals

Martyshuk, T. V.; Gutyj, B. V.; Vyshchur, Oleg; Paterega, I. P.; Kushnir, В. І.; Bigdan, Oleksii; Бушуєва, Інна Володимирівна; Парченко, В. В.; Mykhailiuk, Evhenii; Aleksieiev, Oleksandr; Ткаченко, Н. О.

doi:10.51847/xhwvcyfbz3

Cited by 23 publications

(17 citation statements)

References 15 publications

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“…It has large amounts of vitamins of group B, necessary for the regulation of fat metabolism, nutrition of the cardiac muscle, nervous system, skin, eyesight organs, and also fat-solving vitamins -А, F, E and К. Also, the seeds contain the following macroelements (mg/g): potassium -9.2, calcium -16.6, magnesium -4.2, ferrum -0.08; microelements (µg/g): cuprum -1.16, manganese -0.10, zinc -0.71, chromium -0.15, selenium -22.9, iodine -0.09, boron -22.4 (Martyshuk et al, 2020(Martyshuk et al, , 2022Dang et al, 2022). Fruits of Silybum marianum (L.) Gaertn.…”

Section: Discussionmentioning

confidence: 99%

Effect of liposomal drug based on interferon and extract from Silybum marianum on antioxidative status of bulls against the background of contamination of fodders by cadmium and plumbum

Gutyj

Martyshuk

Парченко

et al. 2022

Regul. Mech. Biosyst.

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The balance between anti- and prooxidants in animal organisms in general and in each cell in particular is responsible for the regulation of many metabolic processes that provide immunocompetence, growth, development and protection of animals from oxidative stress, related to inflow of cadmium and plumbum. Therefore, the objective of our study was the influence of a liposomal drug based on interferon and milk thistle (Silybum marianum (L.) Gaertn.) on the antioxidative status of the organism of bulls in the conditions of cadmium and plumbum loading. The experiments were performed on six-months-old Black Motley dairy cattle. The fodders in the farm were determined to contain high levels of plumbum and cadmium. The liposomal drug Lipointersyl inhibited the lipid peroxidation processes in the bulls. The drug components promoted the decrease in the level of intermediate and end products of lipid peroxidation, in particular 22% decrease in the level of diene conjugates and 20% decrease in TBA-active products. Intramuscular injection of the liposomal drug to bulls of the experimental group strengthened the antioxidant protection of their organism. On the 30th day of the experiment, blood from experimental group animals was seen to have a 9.8% increase in reduced glutathione. Assay of the enzymatic link of the glutathione system revealed that the activity of glutathione peroxidase and glutathione reductase in the blood of the animals that had been injected the liposomal drug Lipointensyl had increased by 24.0% and 27.7% respectively by the 30th day of the experiment. The experiments conducted on young cattle demonstrated that intermuscularly injected the Lipointensyl liposomal drug – against the background of cadmium and plumbum loading – promoted the activation of the glutathione system of antioxidant protection as a result of increase in the activity of its enzymatic and non-enzymatic links. The study of catalase and superoxide dismutase activities revealed that on the 30th and 40th days of the experiment, the activity of those enzymes varied within the physiological norms. Therefore, the analyzed Silybum marianum-based liposomal drug has antioxidant properties, it is recommended for young cattle in the conditions of contamination with heavy metals in order to prevent the development of oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Effect of liposomal drug based on interferon and extract from Silybum marianum on antioxidative status of bulls against the background of contamination of fodders by cadmium and plumbum

Gutyj

Martyshuk

Парченко

et al. 2022

Regul. Mech. Biosyst.

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“…Після закінчення експерименту лабораторних тварин за умов легкого ефірного наркозу декапітували, відбирали кров для гематологічних і біохімічних досліджень та визначали вагові коефіцієнти маси внутрішніх органів (Sachuk et al, 2021;Vasylyev et al, 2021;Martyshuk et al, 2022;Karpenko et al, 2022). Для гематологічних досліджень використовували кров стабілізовану ЕДТА, а для біохімічних досліджень -сироватку крові.…”

Section: матеріал і методи дослідженьunclassified

Comparative assessment of different methods of studying skin toxicity of powder for wounds

Kushnir

Gutyj

et al. 2023

SMLNUVMB

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The article provides data on the comparative assessment of the study of acute and subacute dermal toxicity of powder for wounds made based on iodoform by various methods. The research was conducted using the classic method and according to the methodology of the Organization for Economic Cooperation and Development (OECD No. 410). When studying acute skin toxicity using the traditional method, it was established that the drug under study belongs to the IV toxicity class (low-toxic substances) and, when determined by the way according to the UGS (GHS) – to category 5. During long-term dermal application of powder for wounds, no significant changes in hemoglobin concentration, hematocrit value, the average concentration of hemoglobin in erythrocytes (MCHC), the average volume of erythrocytes (MCV), the average content of hemoglobin in erythrocytes (MCH) and several platelets were established by the classical method. In addition, a slight increase in creatinine, urea, and the activity of ALT and AST was established against a slight decrease in the total protein content. When studying the acute toxicity of powder for wounds following the OECD method No. 410, it was confirmed that long-term dermal application of the investigated agent caused a slight increase in the weight coefficients of the liver, heart, and spleen and a probable increase in the weight coefficients of the kidneys against the background of a decrease in the body weight of animals. Also, in animals of all experimental groups, the number of erythrocytes increased by 0.6 %, 5.1 %, and 10.5 % (Р < 0.05), respectively, and the number of leukocytes decreased by 15.9 % (Р < 0.05), 21.8 % (Р < 0.05), 23.4 % (Р < 0.05), mean erythrocyte volume (MCV) by 2.4 %, 4.2 %, 9.2 % (Р < 0,05). When determining the biochemical indicators of blood serum, it was established that using the research product for 28 days caused a slight decrease in the total protein content in animals of the I, II, and III research groups. In addition, the animals of all experimental groups showed an increase in the level of creatinine, respectively, by – 10.6 % (Р < 0.05), 13.3 % (Р < 0.05), 15.4 % (Р < 0.05), urea at – 3.3 %, 7.3 % (Р < 0.05), 12.6 % (Р < 0.05).

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“…У зв'язку з цим з метою зниження дії стресу під час відлучення актуальним є застосування екологічно чистих та високоефективних препаратів природного походження. Це особливо актуально в найбільш критичні періоди розвитку новонароджених тварин (Polishchuk & Bulavkina, 2010;Ibatullin et al, 2013;Martyshuk et al, 2020Martyshuk et al, , 2022.…”

Section: вступunclassified

Application of Globigen Jump Start feed additive in feeding lactating sows and piglets

Prudyus,

Kyryliv

2023

SMLNUVMB

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Modern methods of pig breeding increasingly use various immunotropic, probiotic agents and feed additives that affect the intestinal microbiota when used in feeding farrowing and lactating sows. The research was conducted in the conditions of a pig farm in the Kyiv region. The number of sows at the pig complex is 500 heads, PIC genetics. Clinically healthy sows with 2–3 farrowing and without rest with similar performance were selected for the study. Two groups of sows were formed by analogs, which were kept in one farrowing ward 5 days before farrowing, with 8 heads in each of the control and experimental groups. For the experiment on sows and piglets in the early rearing period, the Globigen Jump Start feed additive was used. We studied the effect of feeding Globigen Jump Start feed additive, which contains specific lgY immunoglobulins in rations for gilt sows and piglets in the early growing period in the amount of 1 kg and 2 kg per ton of compound feed, respectively. As a result of research, it was established that feeding the drug mentioned above to sows contributed to better preservation of the live weight of lactating sows by 20% compared to sows of the control group. Under the influence of the addition of a feed additive, the quality of sow milk improved. In particular, there was a tendency to increase certain amino acids (cystine, valine, isoleucine, arginine, lysine, and glutamic acid) and some macro- and microelements (magnesium, zinc, sodium, phosphorus, and slightly calcium). Adding the Globigen Jump Start feed additive to piglets' diet increased average daily gains by 18.52%, a decrease in diarrhea by 45.83%, and better feed conversion.

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Study of Acute and Chronic Toxicity of "Butaselmevit" on Laboratory Animals

Cited by 23 publications

References 15 publications

Effect of liposomal drug based on interferon and extract from Silybum marianum on antioxidative status of bulls against the background of contamination of fodders by cadmium and plumbum

Effect of liposomal drug based on interferon and extract from Silybum marianum on antioxidative status of bulls against the background of contamination of fodders by cadmium and plumbum

Comparative assessment of different methods of studying skin toxicity of powder for wounds

Application of Globigen Jump Start feed additive in feeding lactating sows and piglets

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