Study of a region on wheat chromosome 5BS by targeted sequencing

Abstract

Despite the increasing use of high-throughput sequencing resulting from reduced cost and effort, large and complex genomes still pose a challenge in crop genomics. Bread wheat has a genome size of over 17 Gb, which makes targeted capture an invaluable tool for a wide range of studies. To capture and sequence the 26.6-Mb wheat chromosome 5BS region associated to leaf rust resistant genes, custom hybridization probes was created using information from the reference wheat genome ('Chinese Spring'). We show that this chromosomal region of leaf rust resistance and susceptible wheat lines were efficiently captured with sufficient coverage.