Study of 3D-printed chitosan scaffold features after different post-printing gelation processes

Bergonzi, Carlo; Natale, Antonina Di; Zimetti, F.; Marchi, Cinzia; Bianchera, Annalisa; Bernini, Franco; Silvestri, Marco; Bettini, Ruggero; Elviri, Lisa

doi:10.1038/s41598-018-36613-8

Cited by 61 publications

(49 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While most of the studies found in the literature have focused mainly on biocompatibility of 3D printed CS, [41][42][43][44][45][46] utilizing it as a toughening bio-based filler for robust 3D printed composites has yet to be explored. Therefore, inspired by the concept of ionic complexation, we report the preparation and characterization of a SMCS additive where CS was compatibilized by surfactant, sodium dodecyl sulfate (SDS), before incorporation into polymethacrylate (PMA)-based SLA resin.…”

mentioning

confidence: 99%

On the Use of Surfactant‐Complexed Chitosan for Toughening 3D Printed Polymethacrylate Composites

Maalihan

Chen

Agueda

et al. 2020

Macro Materials & Eng

View full text Add to dashboard Cite

This work reports a simple approach to prepare toughened 3D‐printed polymethacrylate (PMA) composites using surfactant‐modified chitosan (SMCS) particles at loadings between 2–10 wt%. Chitosan (CS) is modified with anionic surfactant, sodium dodecyl sulfate, via ionic complexation to facilitate compatibility and dispersion of CS to PMA matrix by non‐covalent interactions between the components. The study successfully demonstrates high‐accuracy 3D printing of composites with significant improvements in the overall mechanical properties. The composite with the best loading of 8 wt% SMCS shows a tensile modulus of 1.23 ± 0.05 GPa, a tensile strength at 49.8 ± 0.96 MPa, a yield stress at 33.3 ± 1.48 MPa, and a strain‐at‐failure 10.3 ± 0.61%, which are 45%, 40%, 32%, and 68% higher than neat PMA, respectively. This provides a significant improvement in toughness at 4.92 ± 0.55 MJ m−3 for the composite, 184% higher than that of neat PMA. The marked increase in toughness is due to enhanced filler‐matrix interactions which improve the ability of the 3D printed composite to absorb energy under tensile load. The results from this work provide new understandings into the strategies for design and preparation of stereolithography 3D printed materials reinforced with toughening fillers from renewable resources.

show abstract

mentioning

confidence: 99%

On the Use of Surfactant‐Complexed Chitosan for Toughening 3D Printed Polymethacrylate Composites

Maalihan

Chen

Agueda

et al. 2020

Macro Materials & Eng

View full text Add to dashboard Cite

show abstract

“…Ink formulations for 3D printing were developed following the same preparation principles and critical parameters used for scaffold production in previous research works (e.g., viscosity, polymer concentrations, homogeneity) [ 29 , 30 , 31 ].…”

Section: Methodsmentioning

confidence: 99%

Three-Dimensional (3D) Printed Silver Nanoparticles/Alginate/Nanocrystalline Cellulose Hydrogels: Study of the Antimicrobial and Cytotoxicity Efficacy

et al. 2020

Self Cite

View full text Add to dashboard Cite

Here, a formulation of silver nanoparticles (AgNPs) and two natural polymers such as alginate (ALG) and nanocrystalline cellulose (CNC) was developed for the 3D printing of scaffolds with large surface area, improved mechanical resistance and sustained capabilities to promote antimicrobial and cytotoxic effects. Mechanical resistance, water content, morphological characterization and silver distribution of the scaffolds were provided. As for applications, a comparable antimicrobial potency against S. aureus and P. aeruginosa was demonstrated by in vitro tests as function of the AgNP concentration in the scaffold (Minimal Inhibitory Concentration value: 10 mg/mL). By reusing the 3D system the antimicrobial efficacy was demonstrated over at least three applications. The cytotoxicity effects caused by administration of AgNPs to hepatocellular carcinoma (HepG2) cell culture through ALG and ALG/CNC scaffold were discussed as a function of time and dose. Finally, the liquid chromatography-mass spectrometry (LC-MS) technique was used for targeted analysis of pro-apoptotic initiation and executioner caspases, anti-apoptotic and proliferative proteins and the hepatocyte growth factor, and provided insights about molecular mechanisms involved in cell death induction.

show abstract

“…Chitosan is a linear cationic polysaccharide formed from deacetylation of chitin which is a natural component of crustacean and insect exoskeleton [75]. Chitosan which has been approved by Food and Drug Administration (FDA) is a compatible biomaterial because of its non-cytotoxicity, biocompatibility, biodegradability, and antibacterial characteristic [75,76]. Also, chitosan has amino groups and secondary and primary hydroxyl groups for the copolymer synthesis [77].…”

Section: Treatment For Degenerative Ivdmentioning

confidence: 99%

Tissue Engineering Strategies for Intervertebral Disc Treatment Using Functional Polymers

2019

View full text Add to dashboard Cite

Intervertebral disc (IVD) is the fibrocartilage between the vertebrae, allowing the spine to move steadily by bearing multidirectional complex loads. Aging or injury usually causes degeneration of IVD, which is one of the main reasons for low back pain prevalent worldwide and reduced quality of life. While various treatment strategies for degenerative IVD have been studied using in vitro studies, animal experiments, and clinical trials, there are unsolved limitations for endogenous regeneration of degenerative IVD. In this respect, several tissue engineering strategies that are based on the cell and scaffolds have been extensively researched with positive outcomes for regeneration of IVD tissues. Scaffolds made of functional polymers and their diverse forms mimicking the macro- and micro-structure of native IVD enhance the biological and mechanical properties of the scaffolds for IVD regeneration. In this review, we discuss diverse morphological and functional polymers and tissue engineering strategies for endogenous regeneration of degenerative IVD. Tissue engineering strategies using functional polymers are promising therapeutics for fundamental and endogenous regeneration of degenerative IVD.

show abstract

Study of 3D-printed chitosan scaffold features after different post-printing gelation processes

Cited by 61 publications

References 22 publications

On the Use of Surfactant‐Complexed Chitosan for Toughening 3D Printed Polymethacrylate Composites

On the Use of Surfactant‐Complexed Chitosan for Toughening 3D Printed Polymethacrylate Composites

Three-Dimensional (3D) Printed Silver Nanoparticles/Alginate/Nanocrystalline Cellulose Hydrogels: Study of the Antimicrobial and Cytotoxicity Efficacy

Tissue Engineering Strategies for Intervertebral Disc Treatment Using Functional Polymers

Contact Info

Product

Resources

About