Study of 16 Portuguese activated sludge systems based on filamentous bacteria populations and their relationships with environmental parameters

Santos, Liliana; Ferreira, Vanessa; Neto, Marta Martins; Pereira, M. A.; Мота, М.; Nicolau, Ana

doi:10.1007/s00253-015-6393-8

Cited by 41 publications

(9 citation statements)

References 22 publications

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“…According to this, Maza-Márquez et al (2015) pointed out that the variables mostly influencing biological ordinations were related to the input of nutrients and the accumulation of biomass in the biological reactors. This is supported by the results of Araújo dos Santos et al (2015) in a survey of 16…”

Section: Effects On Effluent Quality and Degradation Efficiencysupporting

confidence: 57%

“…Apart from this study, four previous works are available which link the incidence and population dynamics of foam causing filamentous bacteria to environmental variables and operational parameters based on multivariate data analysis (Frigon et al 2006;Araújo dos Santos et al 2015;Ju and Zhang 2015;Maza-Márquez et al 2015). According to this, Maza-Márquez et al (2015) pointed out that the variables mostly influencing biological ordinations were related to the input of nutrients and the accumulation of biomass in the biological reactors.…”

Section: Effects On Effluent Quality and Degradation Efficiencymentioning

confidence: 91%

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Illumina sequencing for the identification of filamentous bulking and foaming bacteria in industrial activated sludge plants

Dunkel

Gallegos

Böck

et al. 2017

Int. J. Environ. Sci. Technol.

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In this study, Illumina sequencing was used for the identification of bulking and foaming bacteria in industrial wastewater treatment plants. The reliable identification of bulking and foaming bacteria represents the first step in developing effective and specific control strategies to avoid disturbances in activated sludge systems. Illumina sequencing revealed 432 16S rRNA operational taxonomic units, representing phylotypes and including 21 bulking and foaming bacteria in the two investigated industrial wastewater treatment plants. Foaming represents the most severe problem in the cascade biology system. Up to 22.5% of all sequencing reads are bulking and foaming bacteria, including Chryseobacterium, Candidatus Microthrix parvicella and Gordonia sp. as the dominant bulking and foaming bacteria which are known for foam formation. Moreover, Illumina sequencing revealed an increase in Candidatus Microthrix parvicella and Gordonia sp. reads from activated sludge to foam and scum samples, indicating a preferred flotation and/or growth advantages in the foam and scum layers. Analyses of the taxonomic assignment and distribution showed that the phylum Actinobacteria is the most dominant phylum, underlining the key role of Actinobacteria in bulking and foaming. Multivariate data analysis was applied, revealing that the dominant bulking and foaming bacteria are positively correlated with the sludge age and influent flow and negatively correlated with the dissolved oxygen level and the temperature. In terms of developing a specific control strategy, the positive linear relationships to the fatty acid and surfactant sludge loadings are highlighted and the removal of lipid compounds from the wastewater influent could avoid an overgrowth of bulking and foaming bacteria. Keywords CandidatusMicrothrix parvicella Á Filamentous bacteria Á Filamentous foaming Á Illumina sequencing Á Next-generation sequencing Á Industrial activated sludge plants Editorial responsibility: BV Thomas.

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Section: Effects On Effluent Quality and Degradation Efficiencysupporting

confidence: 57%

Section: Effects On Effluent Quality and Degradation Efficiencymentioning

confidence: 91%

Illumina sequencing for the identification of filamentous bulking and foaming bacteria in industrial activated sludge plants

Dunkel

Gallegos

Böck

et al. 2017

Int. J. Environ. Sci. Technol.

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“…A later examination of 128 samples taken from 16 Portuguese plants over a 2 year period (Dos Santos et al, 2015) used FISH only to distinguish between filaments with indistinguishable microscopic morphologies, and so the data they presented were based largely on the Eikelboom/Jenkins methods, with all their limitations (see earlier). Again “ Ca .…”

Section: Impact Of Molecular Tools On Understanding the Bulking And Fmentioning

confidence: 99%

The Phylogeny, Biodiversity, and Ecology of the Chloroflexi in Activated Sludge

et al. 2019

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It is now clear that several of the filamentous bacteria in activated sludge wastewater treatment plants globally, are members of the phylum Chloroflexi. They appear to be more commonly found in treatment plants designed to remove nitrogen (N) and phosphorus (P), most of which operate at long sludge ages and expose the biomass to anaerobic conditions. The Chloroflexi seem to play an important beneficial role in providing the filamentous scaffolding around which flocs are formed, to feed on the debris from lysed bacterial cells, to ferment carbohydrates and to degrade other complex polymeric organic compounds to low molecular weight substrates to support their growth and that of other bacterial populations. A few commonly extend beyond the floc surface, while others can align in bundles, which may facilitate interfloc bridging and hence generate a bulking sludge. Although several recent papers have examined the phylogeny and in situ physiology of Chloroflexi in activated sludge plants in Denmark, this review takes a wider look at what we now know about these filaments, especially their global distribution in activated sludge plants, and what their functional roles there might be. It also attempts to outline why such information might provide us with clues as to how their population levels may be manipulated, and the main research questions that need addressing to achieve these outcomes.

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“…The development of culture-independent molecular methods has facilitated more reliable identification and description of the diversity of filamentous bacteria present in the activated sludge environment. Fluorescence in situ hybridization (FISH) and 16S rRNA amplicon sequencing have been applied to survey microbial communities in activated sludge to identify and characterize filamentous bacteria common in this ecosystem (Mielczarek et al, 2012;Araújo Dos Santos et al, 2015;Milobędzka and Muszyński, 2015;Jiang et al, 2016;Saunders et al, 2016;Wang et al, 2016;Zhang et al, 2019). Filamentous genera found therein span several phyla, i.e., Actinobacteria (Rossetti et al, 2005), Bacteroidetes (Van Veen et al, 1973;Kragelund et al, 2008), Chloroflexi (Speirs et al, 2009;McIlroy et al, 2016), Firmicutes (Liu et al, 2000), Planctomycetes and Proteobacteria (Kragelund et al, 2005(Kragelund et al, , 2006.…”

Section: Introductionmentioning

confidence: 99%

Candidatus Amarolinea and Candidatus Microthrix Are Mainly Responsible for Filamentous Bulking in Danish Municipal Wastewater Treatment Plants

et al. 2020

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Filamentous bulking is a common serious operational problem leading to deteriorated sludge settling that has long been observed in activated sludge biological wastewater treatment systems. A number of bacterial genera found therein possess filamentous morphology, where some have been shown to be implicated in bulking episodes (e.g., Ca. Microthrix), the impact of many others is still not clear. In this study we performed a survey of 17 Danish municipal wastewater treatment plants (WWTPs) with nutrient removal using 16S rRNA amplicon sequencing over a period of 13 years, where all known filamentous bacteria from 30 genera were analyzed. The filamentous community constituted on average 13 ± 6%, and up to 43% of total read abundance with the same genera common to all plants. Ca. Microthrix and several genera belonging to phylum Chloroflexi were among the most abundant filamentous bacteria. The effect of filamentous bacteria on sludge settling properties was analyzed using measurements of the diluted sludge volume index (DSVI). Strong positive correlations with DSVI were observed only for Ca. Microthrix and Ca. Amarolinea, the latter being a novel, recently characterized genus belonging to the phylum Chloroflexi. The bulking potential of other filamentous bacteria was not significant despite their presence in many plants. Low phylogenetic diversity was observed for both Ca. Microthrix and Ca. Amarolinea, making physiological characterization of individual species and potential development of control strategies more feasible. In this study we show that, despite the high diversity of filamentous phylotypes in Danish WWTPs, only few of them were responsible for severe bulking episodes.

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Study of 16 Portuguese activated sludge systems based on filamentous bacteria populations and their relationships with environmental parameters

Cited by 41 publications

References 22 publications

Illumina sequencing for the identification of filamentous bulking and foaming bacteria in industrial activated sludge plants

Illumina sequencing for the identification of filamentous bulking and foaming bacteria in industrial activated sludge plants

The Phylogeny, Biodiversity, and Ecology of the Chloroflexi in Activated Sludge

Candidatus Amarolinea and Candidatus Microthrix Are Mainly Responsible for Filamentous Bulking in Danish Municipal Wastewater Treatment Plants

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