Study Molecular and Immunological Techniques for Detection of Leishmania Tropical, Major in Baghdad , Iraq

Abstract

The study was conducted on 444 patients suffering from skin lesions of different ages and genders that were diagnosed by dermatologists as cutaneous leishmaniasis for people reviewing and throughout the days of the week for the department of epidemiology and transitional diseases who attended to hospitals in the city of Baghdad, namely (Al-Shaheed Dari Al Fayyad Teaching Hospital , Al-Shaheed Al Sadr Hospital , Al-Numan Teaching Hospital, Al-Karama Teaching Hospital) for the study period from October 1, 2019 until the end of March 2020.
 The methods of molecular diagnostics showed that multiplex pcr examined40 random samples of the parasite that were collected throughthis technique from blood samples taken from the ulcers of infectedpeople to reveal the DNA parts of the Leishmania parasite. Specialprefixes for the Leishmania parasite species showed that 28 positive samplescontain the parasite's DNA and 12 negative samples, of which 11 are forthe Leishmania tropica type (27.5%) and 17 samples for the Leishmania major type(42.5%) at the molecular weight 1150 base pair.
 30 serum samples were taken from patients diagnosed with cutaneous leishmaniasis for serological tests. The current study showed a significant decrease in immunoglobulin G for patients with cutaneous leishmaniasis by 1205.100 ± 210.155 , The current results also showed a significant decrease in the level of immunoglobulin M in patients with cutaneous leishmaniasis by 90.366 ± 26.181

Preparation and Spectral Study of Some Metal(II)-Azo Complexes With Bidentate Azo Ligand

“…The band, appearing at (1651 cm −1 ) due to (-C=N-) group of oximinate moiety (17) , remains unaffected in the spectra of complexes which clearly indicates its non-participation in the process of complexation (18,19) . The spectrum of the ligand shows absorption band at(1600 cm −1 ) due to (-C=N-) of the N3 imidazole nitrogen, its observed with a little change in shape and shifted to lower frequencies (1575-1616 cm −1 ) in the complexes spectra these differences suggest the linkage of metal ion with nitrogen imidazole ring (20)(21) . A band, observed at (1445 cm −1 ) in the ligand spectrum, due to (-N=N-) group appearing in the region (1440-1467 cm −1 ) with different in shape and reduced in intensity in the spectra of prepared complexes, confirming involvement of azo-nitrogen in bonding with metal ions (22) .…”

Morphological Identification and Distribution of Freshwater Snails (Gastropods: Mollusca) in Greater Zab River, Kurdistan Region, Iraq

“…The most widespread species on the margins of lakes and streams and possess a file such as radula which feed on detritus, graze on macrophyte or cobble periphyton, or even float upside down on the surface of the water assisted by surface tension (Dey, 2007;Brown and Lydeard, 2009). The first investigation on the distribution of some mollusks was done in Iraq by Najim (1959), who collected various species of freshwater mollusks from different parts that gave an important note on the distribution of mollusks, after that various species were recorded in different geographical regions in Iraq by several researchers (Harris, 1965;Shamsuddin and Al-Adhami, 1969;Ali, 1979;Radawy, 1979;Mohammad, 1983;Al-Dabbagh and Daoud, 1985;Al-Dabbagh and Luka, 1986;Al-Ali, 2002;Al-Qarooni, 2005;Plaziat and Younis, 2005;Al-Daoody, 2006;Ali et al, 2007;Glöer and Naser, 2007;Ali et al, 2008;Farid et al, 2008;Al-Waaly et al, 2014;Al-Shammari, 2015;Mohammad, 2015;Jaweir and Abid-Ali, 2015;Ghulam and Abdul-Sahib, 2015;Hashim and Al-Taee, 2015;Al-Abbad et al, 2015;Al-Waaly et al, 2015). In contrast, the information about freshwater snails in Kurdistan Region is very scarce, only there are three reports on freshwater snails were conducted in Greater Zab River (Abdullah and Abbas, 1989;Ali, 2007;Ali and Jiwar, 2007).…”

Effect of intestinal parasites infection on clinical features of patients infected with COVID-19 in Al-Diwaniyah governorate

“…The effects of the spread of the Corona virus globally, seems a national and international inability to confront it, a lot of questions and concerns about the efficiency of countries, the feasibility of the international globalization system 1 . National health systems stood in a cohesive, rich and scientifically developed country Unable to fight this virus, to the point of collapse of some of these systems, while the role of the modern international cooperation system has almost disappeared, by the institutions and organizations of the United Nations, it has become very questionable, as was the case of the World Health Organization 2 .It was likely that intestinal parasites were known, especially worms to prehistoric times, Ascaris worms have been diagnosed since 1122 BC in Peru, these worms were also known in Brazil in the period between 2660-2430 BC, it was recorded in Egypt during the period between 2938-2600 BC 3 . Intestinal parasites cause many diseases in humans, sometimes it leads to the death of a person, led to the study and knowledge of intestinal parasites from ages to the present 4 .…”