Study by optical techniques of the dependence of aggregation parameters of human red blood cells on their deformability

Ermolinsky, Petr B.; Луговцов, А.Е.; Priezzhev, Alexander V.

doi:10.18287/jbpe20.06.020305

Cited by 5 publications

(2 citation statements)

References 20 publications

(26 reference statements)

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“…They observed a weak yet clear correlation between aggregation and deformability in healthy donor erythrocytes. The analogous result was observed in the recent study [27] when the RBC deformability was impaired artificially using glutaraldehyde and/or osmolarity changes. Interestingly, the interrelation between aggregation and deformation of RBCs almost disappears in the case of membranopathies of erythrocytes occurring in some severe hereditary diseases.…”

Section: Introductionsupporting

confidence: 88%

Probing Red Blood Cell Membrane Microviscosity Using Fluorescence Anisotropy Decay Curves of the Lipophilic Dye PKH26

Semenov

Gvozdev

Moysenovich

et al. 2022

IJMS

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Red blood cell (RBC) aggregation and deformation are governed by the molecular processes occurring on the membrane. Since several social important diseases are accompanied by alterations in RBC aggregation and deformability, it is important to develop a diagnostic parameter of RBC membrane structural integrity and stability. In this work, we propose membrane microviscosity assessed by time-resolved fluorescence anisotropy of the lipophilic PKH26 fluorescent probe as a diagnostic parameter. We measured the fluorescence decay curves of the PKH26 probe in the RBC membrane to establish the optimal parameters of the developed fluorescence assay. We observed a complex biphasic profile of the fluorescence anisotropy decay characterized by two correlation times corresponding to the rotational diffusion of free PKH26, and membrane-bounded molecules of the probe. The developed assay allowed us to estimate membrane microviscosity in the range of 100–500 cP depending on the temperature, which paves the way for assessing RBC membrane properties in clinical applications as predictors of blood microrheological abnormalities.

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Section: Introductionsupporting

confidence: 88%

Probing Red Blood Cell Membrane Microviscosity Using Fluorescence Anisotropy Decay Curves of the Lipophilic Dye PKH26

Semenov

Gvozdev

Moysenovich

et al. 2022

IJMS

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show abstract

“…Erythrocytes exhibit low autofluorescence emission, that originates mainly from hemoglobin photo-products [49]. The cell separation in the blood is, however, currently not realized in vivo by measuring through the skin and these investigations are limited to in vitro studies using different optical methods [50][51][52].…”

Section: Assessment Of Blood Capillaries By Two-photon Excited Fluore...mentioning

confidence: 99%

Correlation between Blood Flow and Various Physiological Parameters in Human Skin

Lademann

Darvin

Meinke

et al. 2022

J-BPE

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Blood flow is an important parameter of the human organism and skin physiology. It correlates with skin temperature, penetration of active substances through the skin, delivery of antioxidants and development of disease symptoms, especially during inflammatory processes. Dr. Alexander Vasilyevich Priezzhev played a major role in the investigation of blood circulation, cell aggregation and disease correlation, as well as in the development of an aggregometer to determine the rheology of human blood flow. The following article provides an overview of the work performed at the

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Influence of optical clearing agents on the scattering properties of human nail bed and blood microrheological properties: In vivo and in vitro study

Moldon,

Ermolinskiy,

Lugovtsov

et al. 2024

Journal of Biophotonics

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Optical clearing agents (OCAs) are substances that temporarily modify tissue's optical properties, enabling better imaging and light penetration. This study aimed to assess the impact of OCAs on the nail bed and blood using in vivo and in vitro optical methods. In the in vivo part, OCAs were applied to the nail bed, and optical coherence tomography and optical digital capillaroscopy were used to evaluate their effects on optical clearing and capillary blood flow, respectively. In the in vitro part, the collected blood samples were incubated with the OCA and blood aggregation properties were estimated using diffuse light scattering techniques. The results indicate that OCAs significantly influence the optical properties of the nail bed and blood microrheology. These findings suggest that OCAs hold promise for improving optical imaging and diagnostics, particularly for nail bed applications, and can modify blood microrheology.

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Study by optical techniques of the dependence of aggregation parameters of human red blood cells on their deformability

Cited by 5 publications

References 20 publications

Probing Red Blood Cell Membrane Microviscosity Using Fluorescence Anisotropy Decay Curves of the Lipophilic Dye PKH26

Probing Red Blood Cell Membrane Microviscosity Using Fluorescence Anisotropy Decay Curves of the Lipophilic Dye PKH26

Correlation between Blood Flow and Various Physiological Parameters in Human Skin

Influence of optical clearing agents on the scattering properties of human nail bed and blood microrheological properties: In vivo and in vitro study

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