Study and evaluation of Wondfo rapid diagnostic kit based on nano-gold immunochromatography assay for diagnosis of Plasmodium falciparum

Peng, Yunping; Wu, Junlin; Wang, Jihua; Li, Wenmei; Yu, Shujuan

doi:10.1007/s00436-011-2643-6

Cited by 14 publications

(6 citation statements)

References 9 publications

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“…Whereas low parasite density might be the most important limiting factor for microscopic diagnosis [6,28], the varying skills and experience of microscopists may also be responsible for the lower sensitivity of diagnosis [29]. In the past, the Pf/Pan test (Wondfo) has been evaluated for diagnosis of P. falciparum only and showed highly satisfactory result [30]. Recently, a CareStart™ kit (Pf/Pan) was evaluated in the nearby Yunnan province of China, which showed comparable results to microscopy for diagnosing P. falciparum and P. vivax infections [31].…”

Section: Discussionmentioning

confidence: 99%

Performance of two rapid diagnostic tests for malaria diagnosis at the China-Myanmar border area

Yan

Wei

et al. 2013

Malar J

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BackgroundRapid diagnostic tests (RDTs) have become an essential tool in the contemporary malaria control and management programmes in the world. This study aims to evaluate the performance of two commonly used RDTs for malaria diagnosis in the China-Myanmar border area.MethodsA total 606 febrile patients in the China-Myanmar border were recruited to this study and were diagnosed for malaria infections by microscopy, two RDTs tests (Pf/Pan device, and Pv/Pf device) and nested PCR.ResultsMalaria parasites were found in 143 patients by microscopy, of which 51, 73, and 19 were Plasmodium falciparum, Plasmodium vivax and P. falciparum/P. vivax mixed infections, respectively. Compared to microscopy, the sensitivity of the Pf/Pan device was 88.6% for P. falciparum and 69.9% for P. vivax with the specificity of 90.4%. For a subset of 350 patients, the sensitivity of the Pf/Pan device and Pv/Pf device for detection of P. falciparum was 87.5% and 91.7%, respectively; and for detection of P. vivax was 72.0% and 73.8%, respectively. The specificity of the Pf/Pan device and Pv/Pf device was 94.3% and 96.5%, respectively. Nested PCR detected malaria parasites in 174 of 606 samples, of which 67, 79, two and 26 were P. falciparum, P. vivax, P. ovale and P. falciparum/P. vivax mixed infections, respectively. Compared to nested PCR, all other methods had sensitivity below 80%, suggesting that a significant number of cases were missed.ConclusionsCompared to PCR, both microscopy and RDTs had lower sensitivities. RDTs had similar performance to microscopy for P. falciparum diagnosis, but performed worse for P. vivax diagnosis. Other RDT products should be selected with higher sensitivity (and good specificity) for both P. falciparum and P. vivax diagnosis.

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Section: Discussionmentioning

confidence: 99%

Performance of two rapid diagnostic tests for malaria diagnosis at the China-Myanmar border area

Yan

Wei

et al. 2013

Malar J

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“…It can be observed from the table that the sensitivity and selectivity of RDTs significantly vary over different studies and thus there is a need for more sensitive, selective, and reliable methods for rapid detection of malaria. One such effort was made by Peng et al [153] by developing the Wondfo rapid diagnostic test which is a nanogold based immunochromatography assay that uses monoclonal antibodies. It is a more rapid and sensitive parasite detection method and results showed very good concordance with microscopy examination with a sensitivity and specificity of 95.49% and 99.53%, respectively.…”

Section: Detection Of Malariamentioning

confidence: 99%

Potential Biomarkers and Their Applications for Rapid and Reliable Detection of Malaria

Jain

Chakma

Patra

et al. 2014

BioMed Research International

109

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Malaria has been responsible for the highest mortality in most malaria endemic countries. Even after decades of malaria control campaigns, it still persists as a disease of high mortality due to improper diagnosis and rapidly evolving drug resistant malarial parasites. For efficient and economical malaria management, WHO recommends that all malaria suspected patients should receive proper diagnosis before administering drugs. It is thus imperative to develop fast, economical, and accurate techniques for diagnosis of malaria. In this regard an in-depth knowledge on malaria biomarkers is important to identify an appropriate biorecognition element and utilize it prudently to develop a reliable detection technique for diagnosis of the disease. Among the various biomarkers, plasmodial lactate dehydrogenase and histidine-rich protein II (HRP II) have received increasing attention for developing rapid and reliable detection techniques for malaria. The widely used rapid detection tests (RDTs) for malaria succumb to many drawbacks which promotes exploration of more efficient economical detection techniques. This paper provides an overview on the current status of malaria biomarkers, along with their potential utilization for developing different malaria diagnostic techniques and advanced biosensors.

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“…Recent interest in using gold nanoparticles (GNPs) in medicine has altered the methods of diagnosis and treatment (Chen et al 2008;Peng et al 2012). However, gold has a long history of use in the western world as nervine, a substance that could revitalize people suffering from nervous conditions.…”

Section: Introductionmentioning

confidence: 99%

Impact of gold nanoparticles on brain of mice infected with Schistosoma mansoni

et al. 2015

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Schistosomiasis is a condition characterized by high rates of morbidity and cognitive impairment. It afflicts many people in tropical and sub-tropical countries. Our study aimed to investigate the protective role of gold nanoparticles (GNPs) on the brain of mice infected with Schistosoma mansoni. Characterizations of GNPs were determined by using high-resolution transmission electron microscopy. Three doses of GNPs (0.25, 0.5, and 1.0 mg/kg body weight) were used to treat animals after S. mansoni infection. The infection induced impairments in histological picture as a result of schistosome infection resulting in a disturbance in the content of the brain neurotransmitters, norepinephrine (NE), and dopamine (DA). Also, the infection induced significant reduction in glutathione level; oppositely, the levels of nitric oxide and malondialdehyde were increased significantly. In addition, S. mansoni was able to disregulate the infected mice brain Cacnb4, Cabp4, Vdac3, Glrb, and Adam23 messenger RNA (mRNA). On the other hand, treatment of mice with GNPs could alleviate the histological impairments, the changes in the content of NE and DA, and the brain oxidative damage. Also, GNPs could regulate the gene expression due to S. mansoni infection. Generally, GNPs could decrease the neurooxidative stress and regulated the gene expression in the brain of infected mice. Consequently, our results revealed an anti-neuroschistosomal effect of GNPs in mice infected with S. mansoni.

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Study and evaluation of Wondfo rapid diagnostic kit based on nano-gold immunochromatography assay for diagnosis of Plasmodium falciparum

Cited by 14 publications

References 9 publications

Performance of two rapid diagnostic tests for malaria diagnosis at the China-Myanmar border area

Performance of two rapid diagnostic tests for malaria diagnosis at the China-Myanmar border area

Potential Biomarkers and Their Applications for Rapid and Reliable Detection of Malaria

Impact of gold nanoparticles on brain of mice infected with Schistosoma mansoni

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