Study About HPLC Method Elaboration and Validation for Acid Lactic Assay in Grape Juice Fermentation Process

Abstract

In the present study, a simple and fast HPLC procedure has been developed for determination of a wide range of lactic acid concentrations. During the lactic acid analysis, the analytic column,YMC-Pack ODS AQ, was operating at 20oC. The mobile phase, 0.03% H3PO4:acetonitrile (88:12), was delivered isocratic, at a flow rate of 0.5 mL/min, in a 25 min run. The method was validate. The method could successfully used to quantify lactic acid in grape juice during fermentation. Keywords: Lactic acid, HPLC, grape juice