Studies with Two Gastrin Antisera of Different Specificity for Gastrins I and II

Hansky, J.; Soveny, C.; Korman, M. G.

doi:10.1159/000197528

Cited by 25 publications

(5 citation statements)

References 11 publications

(17 reference statements)

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“…Such behaviour of gastrin antisera perhaps makes it unlikely that an antibody will be raised against one of the molecular species of gastrin, to which it is totally specific. Nevertheless, antisera relatively more specific than those described in this study have been reported (Hansky, Soveny and Korman, 1974).…”

Section: Discussionmentioning

confidence: 48%

Current problems in the measurement of gastrin release. A reproducible measure of physiological gastrin release

Russel

Bloom

Fielding

et al. 1976

Postgraduate Medical Journal

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SummaryThe interpretation of gastrin release is confused because of variation in the technique of the radioimmunoassayof gastrin, the lack of a standard stimulus for the release of gastrin and diversity in the method used to express the results. These problems have been analysed (a) by examining the cross-reactivity of three gastrin antisera and using each of the antisera to measure basal gastrin levels in fasting normal subjects, duodenal ulcer and post-vagotomy patients; (b) by determining a satisfactory stimulus for gastrin release in normal subjects; (c) by examining the results to determine the best method of presenting the data. The different antisera used were found to give different levels of plasma gastrin in the same sample of plasma. This was not related to the cross reactivity of the antisera. An English breakfast was found to be the most satisfactory stimulus for the release ofgastrin. The expression of the results of such a stimulus of gastrin release was affected least by assay variation when the incremental integrated gastrin response was used. It is concluded that the incremental integrated gastrin response to an English breakfast is a satisfactory method for exploring variations in gastrin release.

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Section: Discussionmentioning

confidence: 48%

Current problems in the measurement of gastrin release. A reproducible measure of physiological gastrin release

Russel

Bloom

Fielding

et al. 1976

Postgraduate Medical Journal

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“…However, an amino-terminal fragment of G17 has been found in the serum of patients with ZES , so that measurements made with such antisera cannot be attributed to immunoreactive G17 alone. Hansky et al (1974) found significant differences between duodenal ulcer and normal subjects in fasting serum gastrin measured with two antisera-one of which cross-reacted with the unsulphated form of G17 but not the sulphated form, and the other cross-reacted equally with sulphated and unsulphated G17. The G17 specific antiserum (L6) used in the present study does not cross-react with G34 or with carboxyl-and amino-terminal fragments of G17 and shows almost equal immunoreactivity with sulphated and unsulphated G17 (Dockray and Taylor, 1976).…”

Section: Discussionmentioning

confidence: 86%

Big and little gastrin responses to food in normal and ulcer subjects.

Taylor¹,

Dockray²,

Calam³

et al. 1979

Gut

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SUMMARY In normal, duodenal ulcer, and gastric ulcer subjects the two main forms of gastrin, GI 7 and G34, were estimated by radioimmunoassay in fasting serum and after feeding. Two antisera were used: one showing high specificity for G17, the other specific for the common COOH-terminus of G17 and G34 and so allowing estimation of G34 by difference. Basal G17 was similar in gastric ulcer, duodenal ulcer, and normal subjects and the increases of G17 after feeding were also similar in these groups. In contrast, basal G34 was similar in normal and duodenal ulcer subjects but raised in gastric ulcer subjects. After a meal the G34 concentration in both gastric and duodenal ulcer patients was significantly higher than normal. It is concluded that the higher post-prandial gastrin responses in peptic ulcer that have been previously described are due largely to increased G34.It is established that gastrin is responsible for the fulminating peptic ulceration seen in patients with the Zollinger-Ellison syndrome (Gregory et al., 1967). However, the importance of gastrin in the pathogenesis of peptic ulcer not associated with gastrinoma is unclear. Patients with both duodenal (Trudeau and McGuigan, 1970;Walsh and Grossman, 1973) and gastric ulcer (Korman et al., 1972) have higher than normal gastrin responses to a meal. However, patients with duodenal ulcer tend to have higher than normal basal and maximal rates of gastric acid secretion, whereas gastric ulcer patients have lower than normal basal and maximal acid outputs (Wormsley and Grossman, 1965). Interpretation of these findings is complicated by the fact that gastrin circulates in several different molecular forms, and that frequently the antibodies used in gastrin radioimmunoassays (RIA) are unable to distinguish between these forms (Walsh, 1974;Stadil et al., 1975). After a meal two forms of gastrin predominate in the circulation. These correspond to peptides with 34 amino-acid residues (big gastrin, G34) and 17 amino-acid residues (little gastrin, G17), that have been isolated from human 'Address for correspondence: Dr G. J. Dockray, Physiological Laboratory, University of Liverpool, Liverpool L69 3BX.Received for publication 16 May 1979 gastrinomas and hog antral mucosa Tracy, 1964, 1972). G34 and G17 may be sulphated or unsulphated at the tyrosine residue, which is in the sixth amino-acid position counting unconventionally from the carboxyl-terminus. Other circulating forms of gastrin have been described (big, big gastrin, component I, minigastrin, etc.) but these either do not increase after feeding or occur only in low concentrations and are unlikely to make more than a minor contribution to circulating gastrin biological activity (Stadil et al., 1975). Walsh and co-workers (1976) have studied the actions of G17 and G34 on gastric acid secretion in patients with duodenal ulcer and have shown that circulating G17 is six to eight times more potent than G34 in stimulating acid secretion. A relatively minor increase in the ratio of G17 to G34 could therefore ...

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“…Any comparison of these values is virtually prohibited by the existence of two major variables between different laboratories. First, different antisera were used in each laboratory and the characteristics of these are almost certainly different such that the 'gastrin' concentration of the same sample would differ, dependent upon the antisera used (Hansky, Soveny, and Korman, 1974).…”

mentioning

confidence: 99%

Gastrin response to meals of different composition in normal subjects.

Blair¹,

Greenwell²,

Grund³

et al. 1975

Gut

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SUMMARY The serum gastrin responses and the integrated gastrin responses to eating three meals of very different composition were studied in the same normal subjects on different days. Two meals, a milk meal of 500 ml, and a breakfast of eggs, toast, butter, marmalade, fruit juice and coffee, were eaten at breakfast time. The serum gastrin responses to these meals were compared and contrasted with the concentrations observed when the subjects fasted over the same time of day. A steak meal was eaten at lunch time. There were no significant differences between the mean serum gastrin concentrations to the three meals but each meal produced a significant increase in serum gastrin above fasting levels. When the prefeeding gastrin concentration was subtracted from the gastrin responses then the integrated responses to the steak meal were greater than those to either of the breakfast meals.Considerable variability in response to any one meal was observed within the group of subjects, but those subjects who produced high serum gastrin concentrations to one meal did so to the others. Conversely, a low response to one meal was reflected in low responses to the other two meals. Fasting serum gastrin concentration was correlated with the age of the subject. Repeatability of the response to one meal was tested in two subjects who ate the same meal on four separate occasions showing their responses to be repeatable.In the past decade the radioimmunoassay of gastrin has provided a new and sensitive tool which enables gastroenterologists to measure plasma gastrin concentrations in all manner of conditions, including the Zollinger-Ellison syndrome (Isenberg, Walsh, Passaro, Moore, and Grossman, 1972 (Ganguli and Hunter, 1972).In the quest to understand better the physiology of gastric secretion and to investigate the possible role of gastrin in the aetiology of peptic ulceration, different workers have used a variety of meals to stimulate and study gastrin secretion. The composition of the meals have ranged from two Oxo cubes (Byrnes, Young, Chisholm, and Lazarus, 1970), through a standard breakfast of boiled eggs, fruit juice, toast, marmalade and coffee to the more substantial steak meal and a sweet (Wyllie, Boulos,

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Studies with Two Gastrin Antisera of Different Specificity for Gastrins I and II

Cited by 25 publications

References 11 publications

Current problems in the measurement of gastrin release. A reproducible measure of physiological gastrin release

Current problems in the measurement of gastrin release. A reproducible measure of physiological gastrin release

Big and little gastrin responses to food in normal and ulcer subjects.

Gastrin response to meals of different composition in normal subjects.

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