Studies Regarding the Antibacterial Effect of Plant Extracts Obtained from Epilobium parviflorum Schreb

Abstract

The present study was carried out to develop an experimental endodontic irrigant solution based on plant extracts obtained from Epilobium parviflorum Schreb. that largely replenish the properties of the usual antiseptics used in dentistry. Background: This study investigated the phytochemical contents of plant extracts obtained from Epilobium parviflorum Schreb. and their potential antibacterial activity. Methods: Identification and quantification of biologically active compounds were made by UV field photo spectrometry, adapting the Folin-Ciocalteu test method. Antibacterial activity was tested on pathological bacterial cultures collected from tooth with endodontic infections using a modified Kirby-Bauer diffuse metric method. Results: Polyphenols and flavonoids were present in all plant extracts; the hydroalcoholic extract had the highest amount of polyphenols—17.44 pyrogallol equivalent (Eq Pir)/mL and flavonoids—3.13 quercetin equivalent (Eq Qr)/mL. Plant extracts had antibacterial activity among the tested bacterial species with the following inhibition diameter: White Staphylococcus (16.5 mm), Streptococcus mitis (25 mm), Streptococcus sanguis (27 mm), Enterococcus faecalis (10 mm). Conclusions: All plant extracts contain polyphenols and flavonoids; the antibacterial activity was in direct ratio with the amount of the bioactive compounds.

Anti-putrefying properties of the aqueous extract of fresh leaves of Manihot esculenta (cassava) on dead laboratory Sprague Dawley rats

“…All these procedures were undertaken in a sterilized biosafety hood. The filtrate was subjected to a sterility test using a 3.5g plate count agar, which was weighed accurately and dissolved using 200 ml of hot distilled water (Șachir et al, 2022;Shami et al, 2013). The resulting solution was kept in a water bath at 100 O C to allow good mixing and then autoclaved at 120 O C for 15 minutes.…”

Bay Leaves Extracts as Active Additive for Food Protective Coatings

“…Then, inoculation was carried out, incubating them at 28 ± 2 • C for B. cereus, C. glabrata, S. cerevisiae, and at 37 ± 2 • C for E. coli for a duration of 24 h. After incubation, it was qualitatively determined if there was microbial inhibition through light microscopy (Gram staining for bacteria and simple staining for yeasts). In the second approach, the disk-diffusion method originally introduced by Kirby-Bauer was followed, with minor adjustments made to the procedure described by S , achir et al, 2022 [18]. Briefly, after the inoculation of each culture medium, disks were impregnated with 50 µL of each previously prepared BLE dilution and subsequently incubated under the same conditions as in the first approach.…”

Studying the effect of plant extracts on gram-positive and gram-negative pathogens: Review

“…This is a multifaceted problem that has a significant and catastrophic impact on everyone, including humans and the environment [1]. According to the World Health Organization, this has led to an estimate that in 2050, 10 million people will die from untreatable infections caused by multidrug-resistant bacteria and ineffective antibiotics [2]. Most recent studies suggest alternatives to antibiotics (such as plant extracts, honey, propolis, prebiotics, probiotics, synbiotics and prebiotics) and combined with drug therapy, can be used as an adjunct to standard treatments well, allowing drug sensitivity of bacteria to develop multidrug resistance.…”