Studies on Variability of Plant Tissue Cultures for Alkaloid Production in Catharanthus roseus and Papaver somniferum Callus Cultures

Pétiard, Vincent; Baubault, C.; Bariaud, A.; Hutin, M.; Courtois, Didier

doi:10.1007/978-3-642-70717-9_13

Cited by 13 publications

(5 citation statements)

References 14 publications

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“…Somaclonal variation, a common phenomenon in plant cell cultures, characterized by phenotypic variation of either genetic or epigenetic origin, may be an explanation for the wide range of inhibitor activity found. Accordingly, somaclonal variation can be either an advantage (variability increase) or disadvantage (loss of stability of cell lines), depending on the aim of the study (7)(8)(9). However, it has become apparent that, rather than being an unexplainable aberration in an otherwise uniform cell proliferation process, the appearance of variants in cell cultures may be a routine occurrence for certain types of plants or specific explant sources.…”

Section: Introductionmentioning

confidence: 99%

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Somaclonal variation: a morphogenetic and biochemical analysis of Mandevilla velutina cultured cells

Maraschin

Sugui

Wood

et al. 2002

Braz J Med Biol Res

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Cell cultures of Mandevilla velutina have proved to be an interesting production system for biomass and secondary metabolites able to inhibit the hypotensive activity of bradykinin, a nonapeptide generated in plasma during tissue trauma. The crude ethyl acetate extract of cultured cells contains about 31-to 79-fold more potent anti-bradykinin compounds (e.g., velutinol A) than that obtained with equivalent extracts of tubers. Somaclonal variation may be an explanation for the wide range of inhibitor activity found in the cell cultures. The heterogeneity concerning morphology, differentiation, carbon dissimilation, and velutinol A production in M. velutina cell cultures is reported. Cell cultures showed an asynchronous growth and cells in distinct developmental stages. Meristematic cells were found as the major type, with several morphological variations. Cell aggregates consisting only of meristematic cells, differentiated cells containing specialized cell structures such as functional chloroplasts (cytodifferentiation) and cells with embryogenetic characteristics were observed. The time course for sucrose metabolism indicated cell populations with significant differences in growth and metabolic rates, with the highest biomass-producing cell line showing a cell cycle 60% shorter and a metabolic rate 33.6% higher than the control (F 2 cell population). MALDI-TOF mass spectrometric analysis of velutinol A in selected cell lines demonstrated the existence of velutinol A producing and nonproducing somaclones. These results point to a high genetic heterogeneity in general and also in terms of secondary metabolite content.

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Section: Introductionmentioning

confidence: 99%

“…As an example, we may mention the production of shikonin from Lithospermum erythrorhizon (17), of berberine from Coptis japonica (18), and of ginsenosides from Panax ginseng (19). Thus, the information about somaclonal variability can be considered fundamental to studies concerning the production of secondary metabolites (7).…”

Section: Introductionmentioning

confidence: 99%

Somaclonal variation: a morphogenetic and biochemical analysis of Mandevilla velutina cultured cells

Maraschin

Sugui

Wood

et al. 2002

Braz J Med Biol Res

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“…These are dimeric indole alkaloids that are formed in vivo by condensation of vindoline and catharanthine. The low yield of dimeric indole alkaloids from the plant (approximately 0.0005%) and their consequent high price have stimulated numerous efforts to develop alternative strategies for their production Petiard et al, 1985;. Despite these intense efforts, attempts to produce antitumor alkaloids from C. roseus cell cultures have failed (reviewed by Van der Heijden et al, 1989) and have been attributed to their inability to synthesize the precursor vindoline.…”

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confidence: 99%

A Cytochrome P-450 Monooxygenase Catalyzes the First Step in the Conversion of Tabersonine to Vindoline in Catharanthus roseus

1995

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Hydroxylation at the C-16 position of the indole alkaloid tabersonine has been suggested as the first step toward vindoline biosynthesis in Catbarantbus roseus. Tabersonine 16-hydroxylase (1 6-OH) activity was detected in total protein extracts from young leaves of C. roseus using a nove1 coupled assay system. Enzyme activity was dependent on NADPH and molecular oxygen and was inhibited by CO, clotrimazole, miconazole, and cytochrome c. 16-OH was localized to the endoplasmic reticulum by linear sucrose density gradient centrifugation. These data suggest that 16-OH is a cytochrome P-450-dependent monooxygenase. The activity of 16-OH reached a maximum in seedlings 9 d postimbibition and was induced by light. l h e leaf-specific distribution of 16-OH in the mature plant is consistent with the localization of other enzymes in the tabersonine to vindoline pathway. However, in contrast to enzymes that catalyze the last four steps of vindoline biosynthesis, enzymes responsible for the first two steps from tabersonine (16-OH and 16-O-methyltransfersase) were detected in C. roseus cell-suspension cultures. These data complement the complex model of vindoline biosynthesis that has evolved with respect to enzyme compartmentalization, metabolic transport, and control mechanisms.

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“…Petiard et al [55] studied variability in a strain which had been stable for 8 years in chlorophyll content, growth rate and alkaloid production. Clones, 18-month-old, derived from protoplasts of the stable cell line, were distributed in two populations.…”

Section: Influence Of Plant Materials and Culture Type On Product Formmentioning

confidence: 99%

Cell and tissue cultures ofCatharanthus roseus (L.) G. Don: a literature survey

Heijden

Verpoorte

Hoopen

1989

Plant Cell Tiss Organ Cult

129

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The literature concerning the formation of secondary metabolites in cell and tissue cultures of Catharanthus roseus has been reviewed. Several aspects involved in the formation of secondary metabolites are discussed; e.g. regulation of secondary metabolism, environmental factors influencing secondary metabolism, biosynthesis and enzymology of the products, analysis of product formation, immobilization of cultured cells and stability of cell lines. Some economical aspects of production processes are discussed.

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Studies on Variability of Plant Tissue Cultures for Alkaloid Production in Catharanthus roseus and Papaver somniferum Callus Cultures

Cited by 13 publications

References 14 publications

Somaclonal variation: a morphogenetic and biochemical analysis of Mandevilla velutina cultured cells

Somaclonal variation: a morphogenetic and biochemical analysis of Mandevilla velutina cultured cells

A Cytochrome P-450 Monooxygenase Catalyzes the First Step in the Conversion of Tabersonine to Vindoline in Catharanthus roseus

Cell and tissue cultures ofCatharanthus roseus (L.) G. Don: a literature survey

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